Extracellular Glycolipids of Yeasts: Biodiversity, Biochemistry, and Prospects
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Extracellular Glycolipids of Yeasts: Biodiversity, Biochemistry, and Prospects provides a comprehensive view of the biochemistry, biological activity, and practical application of extracellular glycolipids of yeast. This book brings much-needed clarity to the complex topic of glycolipids and streamlines the rather confusing terminology used for glycolipids. It also provides a wealth of modern data on their composition, structure and properties, biosynthetic pathways, methods of isolation and identification, antifungal activity, and mechanisms of action.
Studies of extracellular glycolipids of yeast now draw the attention of researchers in life science and biotechnology due to numerous recently revealed biological properties of these compounds. These compounds are scientifically and practically promising in medicine and agriculture due to their biosurfactant and fungicidal properties, as well as a number of other biological activities. Extracellular Glycolipids of Yeasts gives researchers studying biochemistry of microorganisms and related biologically active compounds a much-needed guide to the basic data that will aid in these increasingly generative pursuits.
- Provides a clear overview of the basic data on yeast biosurfactants using a simple survey-style approach
- Delivers comprehensive view of biochemistry, biological activity, and practical application of yeasts to aid in their scientific and practical use
- Clarifies and simplifies the complex topic of glycolipids, and its often-confusing terminology
Ekaterina Kulakovskaya
Degrees: Mr.Sci: July 2005 in Biology (Pushchino State University) Ph.D.: November 2006 in Biochemistry (Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences): Antifungal yeast glycolipids. (Supervisors Prof. Kulaev, I.S., and Dr. W.I.Golubev Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences) Positions held: 2005-2008: Junior Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences 2008-Present: Researcher Skryabin Institute of Biochemistry and Physiology of Microorganisms
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Extracellular Glycolipids of Yeasts - Ekaterina Kulakovskaya
Extracellular Glycolipids of Yeasts
Biodiversity, Biochemistry, and Prospects
Ekaterina Kulakovskaya
Tatiana Kulakovskaya
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
Table of Contents
Cover image
Title page
Copyright
Acknowledgments
Introduction
Chapter 1. Structure and Occurrence of Yeast Extracellular Glycolipids
1.1 The Structures of Extracellular Glycolipids of Yeast
1.2 Glycolipid Occurrence in Eumycetes
Chapter 2. Methods for Studying Yeast Extracellular Glycolipids
2.1 Culture Media and Methods for Increasing the Yield of Yeast Extracellular Glycolipids
2.2 Purification Methods
2.3 Thin-Layer Chromatography Systems for Glycolipid Detection
2.4 Chemical Methods
2.5 NMR Spectroscopy and Mass Spectrometry
2.6 Methods for Studying Physicochemical Properties and Antifungal and Membrane-Damaging Activities
2.7 Molecular Biology Methods
Chapter 3. Physicochemical Properties of Yeast Extracellular Glycolipids
3.1 Solubility
3.2 Stability During Storage and thermal Stability
3.3 Molecular Masses
3.4 Surface-Active Properties
3.5 Lactonization and Self-Assembly
3.6 Interaction Between Cellobiose Lipids and Artificial Membranes
Chapter 4. Biological Activities of Extracellular Yeast Glycolipids
4.1 Antifungal Activity of Cellobiose Lipids
4.2 Membrane-Damaging Activity of Cellobiose Lipids
4.3 Biological Activities of MELs and Sophorolipids
4.4 The Biological Activities of Rare Fungal Glycolipids
4.5 The Role of Extracellular Glycolipid for Yeast Producers
Chapter 5. Metabolism of Yeast Extracellular Glycolipids
5.1 Biosynthesis of Extracellular Glycolipids
5.2 Catabolism of Extracellular Glycolipids
Chapter 6. Prospects of Practical Application of Sophorolipids, Cellobiose Lipids, and MELs
6.1 Application as Membranotropic Agents
6.2 Prospects of Application of Yeast Extracellular Glycolipids in Industry, Agriculture, and Medicine
6.3 Commercial Products Based on Yeast Extracellular Glycolipids
Appendix. Selected Techniques of Purification and Assay of Extracellular Yeast Glycolipids
A.1 Methods for Cultivating Producers and Obtaining Glycolipids
A.2 Methods of Antifungal Activity Assay
A.3 Methods of Membrane-Damaging Activity Assay
References
Subject Index
The Index of Generic Names
Copyright
Academic Press is an imprint of Elsevier
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First published 2014
Copyright © 2014 Elsevier Inc. All rights reserved.
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This book and the individual contributions contained in it are protected under copyright by the Publisher (other than as may be noted herein).
Notices
Knowledge and best practice in this field are constantly changing. As new research and experience broaden our understanding, changes in research methods, professional practices, or medical treatment may become necessary.
Practitioners and researchers must always rely on their own experience and knowledge in evaluating and using any information, methods, compounds, or experiments described herein. In using such information or methods they should be mindful of their own safety and the safety of others, including parties for whom they have a professional responsibility.
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ISBN: 978-0-12-420069-2
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Acknowledgments
The experimental part of the work was done in the Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences.
Special thanks to Dr. W.I. Golubev, the discoverer of antifungal activity of cellobiose lipid producers, for providing yeast strains and for fruitful discussion. We are grateful to our colleagues Drs. E.O. Puchkov, A.S. Shashkov, N.E. Nifantiev, A. Zinin, Y. Tsvetkov, A. Grachev, and A. Ivanov for their great experimental contributions to and creative interpretations of the results. We thank Elena Makeeva for her help with preparing the manuscript. This study was supported by the Russian Foundation for Basic Research, Projects Nos. 06-04-48215, 06-04-08253-ofi, and 12-04-32138-mol-a.
Introduction
Microorganisms are characterized by a great diversity of the so-called secondary metabolites, that is, compounds that are not obligatory participants of metabolism but, nevertheless, provide advantages to producers in their survival under unfavorable environmental conditions and competition for ecological niches. Many of these compounds are biologically active and, hence, have good and promising applications in industry, agriculture, and medicine.
Secondary metabolites include the so-called biosurfactants: lipopeptides, glycolipids, fatty acids, neutral lipids, and phospholipids, as well as some amphiphilic biopolymers. These substances are widespread in microorganisms, from bacteria to fungi. They were found during the studies of microbial growth on hydrophobic substrates, including oils and hydrocarbons, and were supposed to improve the solubility and bioavailability of these substrates. The properties of biosurfactants of different chemical nature and origin, as well as their research and commercial prospects, have been described in a number of reviews (Lang and Wagner, 1987; Rosenberg and Ron, 1999; Kitamoto et al., 2002; Rodrigues et al., 2006; Langer et al., 2006; Arutchelvi et al., 2008; Van Bogaert et al., 2007, 2011; Irudaraj et al., 2008). Many reviews are devoted to future potential of biosurfactants in medicine and industry (Banat et al., 2010; Fracchia et al., 2012; Marchant and Banat, 2012; Cortés-Sánchez et al., 2013). Springer Publishers have issued a volume Biosurfactant
in the series Advances in Experimental Medicine and Biology
(Sen, 2010) and a volume Biosurfactants. From Genes to Applications
in the series Microbiology Monographs
(Soberón-Chávez, 2011).
The following properties of these compounds make them relevant for life science and biotechnology:
– structural diversity;
– multiple biological activities;
– biodegradability;
– nontoxicity;
– the possibility of inexpensive production using simple nutrient media, including those containing industrial and agricultural wastes;
– promising applications as detergents, antibiotics, and amphiphilic compounds.
The extracellular glycolipids of yeast and fungi belong to biosurfactants. These compounds are glycosides of fatty acids containing one or more monosaccharide residues that may contain additional O-substituents at the sugar moiety.
These compounds are mentioned in many reviews on biosurfactants (Lang and Wagner, 1987; Rosenberg and Ron, 1999; Kitamoto et al., 2002; Cameotra and Makkar, 2004; Rodrigues et al., 2006; Langer et al., 2006). However, the reviews devoted specifically to yeast extracellular glycolipids are few (Van Bogaert et al., 2007a,b, 2011; Arutchelvi et al., 2008; Bölker et al., 2008; Kulakovskaya et al., 2008, 2009; Arutchelvi and Doble, 2011; Van Bogaert and Soetaert, 2011
The studies of yeast extracellular glycolipids attract attention due to their numerous activities: from biosurfactant properties providing utilization of hydrophobic substrates to fungicidal properties, as well as a number of other biological activities that make these compounds scientifically and practically promising.
Structural diversity, numerous biological activities, biodegradability, nontoxicity, and possibility of inexpensive production make them attractive for future applications in industry, cosmetology, medicine and agriculture as ecologically pure detergents, fungicides of new generation, and other useful products. Up to date, scientific literature has accumulated quite a lot of data on these compounds, which should be generalized for better understanding of the potential of yeast as a producer of biologically active substances, for development of ecological biotechnologies and research reagents. Although the biological role of extracellular glycolipids in nature is associated primarily with their surfactant properties, the detection of antifungal activity against a broad spectrum of yeast-like fungi in cellobiose lipids (representatives of these compounds) suggests that glycolipid secretion may play a key role in the adaptation to unfavorable environmental conditions. The study of structural peculiarities, the mechanism of action, and distribution of these natural fungicides may be important for a better understanding of antagonistic relationship between microorganisms, as well as the prospects of their practical application as compounds for plant and crop protection from phytopathogenic fungi and antibiotics and biologically active compounds in medicine.
Generalization of the data on the biochemistry, cell biology, and biotechnology of extracellular fungal glycolipids is of concern for microbiologists, biochemists, biotechnologists, and students of the respective specialties.
The book presents modern data on the yeasts producing extracellular glycolipids, their composition, structure and properties, biosynthetic pathways, methods of isolation and identification, antifungal activity, and mechanisms of action. The applied potential of these compounds in medicine, agriculture, and industry is being considered. The emphasis is placed on cellobiose lipids, including their structure, distribution, and antifungal activity.
Chapter 1
Structure and Occurrence of Yeast Extracellular Glycolipids
The most-known extracellular glycolipids of yeast fungi are cellobiose lipids, mannosylerythritol lipids (MELs), and sophorolipids. Each of the three groups of glycolipids comprises a set of compounds which differ in the O-substituents in carbohydrate residue and in the structure of fatty acid residue. Sophorolipids are produced by ascoporous yeasts (class Saccharomycetes, order Saccharomycetales) of the genera Starmerella, Wickerhamiella, Wickerhamomyces, and the phylogenetically-related asporogenous species of the genus Candida. The only exception among sophorolipid-secreting yeasts is Rhodotorula bogoriensis which is related to basidiomycetes (class Microbotryomycetes). Cellobiose lipids and MELs are synthesized almost exclusively by basidiomycetes, mainly members of the order Ustilaginales (class Ustilaginomycetes): the species of the genera Pseudozyma and Ustilago. Some producers of cellobiose lipids in basidiomycetes were also found in the classes Exobasidiomycetes (Sympodiomycopsis paphiopedili belonging to the order Microstromatales) and Tremellomycetes (order Trichosporonales, genera Cryptococcus and Trichosporon).
Keywords
Cellobiose lipid; mannosylerythritol lipid; sophorolipid; eumycetes;