HPLC CALCULATION SPREAD SHEET FOR ORAL SOLID DOSAGE FORM

Weight of sample taken = Weight of RS taken =

100 50 First 100 ml

Average / Propsed weight = Potency of RS =

200 99.3 Third 1 ml

Dilution of WS 50 mg

2 ml

Second 50 ml

1 ml

Dilution of Sample 100 mg

First 100 ml

2 ml

Second 50 ml

1 ml

Third 1 ml

Peak Areas of WS

Peak Areas of Sp

Average

10000 10000 10000 10000 10000 10000 10000

10000 10000 10000

10000

Contents of API =

Peak Areas of Sp Peak Areas of WS

x x

Weight of WS Weight of Sp

Diluiton of Sp Dilution of WS

Potency of WS

Avg. / Prop. Wt =

10000 10000

x x 100

50 100

2500 2500

99.3 100

200

Label claim Remarks

mg/Tab complies % = 99.30% Limit 95% ---------------- 105%

mg/Tab

99.3 mg/Tab