Gene: Eukaryotic translation elongation factor 2

Bios 3100 Extra Credit Nicole Kleinas

Summary: Elongation factor 2 is required in protein synthesis to facilitate the translocation of the growing polypeptide chain from the A-site to the P-site. It is located on the short arm of chromosome 19 at position 13.3 (19p13.3). eEF-2 is expressed in all human tissue, since it is required for protein synthesis in all eukaryotic cells. However, it is expressed in some tissues more than others (Figure 1). It is expressed most in the thyroid (other than cancerous tissues). eEF-2 is the target of the diphtheria toxin, which phosphorylates the protein to inactivate it. Without an active elongation factor, cells cannot synthesize proteins and initiating apoptosis. Figure 1.

cDNA sequence:

ctcttccgcc tctacctggg agaccagatc ctgtcatcgc gtgtgcaagg cactgatacc ctgccatctc aagcagagca cgggcatgtc atggcgcatt gagacagtgc gatgaacaag agctctacca tccacctacg tcctgtcctc tcaccctgaa ggggagggcc gatgaagaag tcagcaagtc tgccagctga tttcaagaaa tggacagcga atgcgccgct ccacctgccc acgaggggcc cccaaaggcc caaaggtcgg ctggcctgaa gaggacctct ctacgtggag tgggcgtgga cacgcgcaca agtggccgtg ggctgaagcg gagtcgggag ctgcctgaag ctgacccggt ctctgcctct

gtcgtcgccg agaatccacc cgcgccatca ccacgtggac cgggcatcat cggaaggacg cctcttctac aggacggtgc gacttctcct ggtggtggtg tgcggcaggc atggaccgcg gactttccag gcgagggcga ggtaccgtgg gcagtttgcc agttggggcc ctgtggggtg agccaccagc tcctggaccc gaggagacag ggacaaggac ggctgcctgc tcccctgtga cccggacgac ctcttatgat ttctacgcct ggtcaggatc acctgaagcc cccatcgagg ccagttcctg acatgcgggt gaggccaaga gctggccaag agcatatcat gacctggagg cgtctcgtac ccaagtcccc

ccatcctcgg gccatccgcc tggacaagaa catggcaagt cgcctcggcc agcaggagcg gagctctcgg cggcttcctc cggaggtgac gactgcgtgt cattgccgag ccctgctgga cgcatcgtgg gagcggcccc gctttgggtc gagatgtatg tgccgagcgg acaggtactt cccgaaggga catcttcaag caaaactgat aaagaaggca cggagacgcc cggcccagaa gaggctgcca gtatatttcc ttggacgagt atggggccca aatccagaga atgtgccttg gtgaagacgg gatgaagttc acccggctga tccgacccca cgcgggcgcc aggaccacgc cgcgagacgg caacaagcac

cgcgactcgc accatggtga ggccaacatc ccacgctgac cgggccgggg ttgcatcacc agaatgactt atcaacctca tgctgccctc caggcgtgtg cgcatcaagc gctgcagctg agaacgtgaa atgggcaaca tggcctccac tggccaagtt gccaagaaag tgacccagcc agaagctgcc gtgtttgatg agagaaactg aacccctgct ttgttgcaga gtaccgctgc tgggcattaa aaaatggtgc cttctcgggg actatacccc acaatcttga tgggaacatt gcaccatcac agcgtcagcc cctgcccaag tggtgcagtg ggcgagctgc ctgcatcccc tcagtgaaga aaccggctgt

ttctttcggt acttcacggt cgcaacatgt agactccctg agacacgctt atcaagtcaa gaacttcatc ttgactcccc cgagtcaccg cgtgcagacg ctgtgctgat gagcccgagg cgtcatcatc tcatgatcga gggtgggcct cgccgccaag tagaggacat aacggcaagt acgcaccttc cgatcatgaa gacatcaaac gaaggctgtg tgatcaccat gagctcctgt aagctgtgac caacctccga ctggtctcca tgggaagaag tgatgggccg gtgggcctcg caccttcgag ctgttgtcag ctggtggagg catcatcgag acctggagat atcaagaaat gtcgaacgtg acatgaaggc

gcggcccttc ccgcccgtca gagtgggacg caccggcccc acgagatcaa ggcgcactgt cgtcaccctg ccacagcacg ctcatggagc cggtggcatc agtcccaggt gtcaacgagt ccaggcgttc accccttcga aagcgcaagg caaattgtag gcacccacag cccgacacag atcactcaac agtccggagg cggggagggg gagggaaact tatttgggta acacgcgggt catgagcacc ggtggtgaaa

cccgacggcc ggagctcaag tggctgaggc aacatcctca ggacagtgtg gtgaggagaa cacgccgacg gcgctgcctc ccatctacct tacggggttt ggccggcacc cctttggctt ccccagtgtg caacagcagc gcctgaaaga gcggcccttc caccacgtcc cgacgctccc cataacactt cagcagacac gaggcgggat cagatgtcca catggcccgc agatgatttc tccagctgta aaa

tggccgagga cagcgggcgc ccgcaagatc ccgacatcac gtggccggct catgcggggt ccatccaccg tatgccagtg tgtggagatc tgaacaggaa cccatgtttg caccgctgac tgtttgacca cgccccagcc aggcatccct ctgcagcgcc tcgaattctc ctgagaggtt gatgccgttt gccctcttag gggacaccca aactaatttt agtggctttt tagcaggcag ctagtgccat

catcgataaa gctacctggc tggtgctttg caagggtgtg tccagtgggc gtgcgcttcg cggagggggc tgctgaccgc cagtgtccag gcggggccac tggtcaaggc ctgaggtcca ctggcagatc aggtggtggc gccctggaca tgccgccccg agacgacacc tctggggccc ctttcaatat tagggactta acactttttc aacaaacgca gccccagaaa gaagtcctgt tggaataata

ggcgaggtgt cgagaagtac ggcccgacgg cagtacctca caccaaggag acgtccacga cagatcatcc ccagccacgc agcaggtggt gtgttcgagg ctatctgccc acacgggcgg ctgcccggag ggagacccgc acttcctgga gggactcgca tggagactgt gctgcgtgcc ttatttccag atgggccggt catttcttca ttaagaggtt ggggaaagga gcggtgtcac aatttgataa