Sunteți pe pagina 1din 107

Medicina generala UMF Carol Davila

PROTOZOARE

Asistent universitar NEAGOE IONELA

AMIBE

AMIBE PATOGENE

Entamoeba hystolitica

Entamoeba hystolitica
Stadiul de CHIST

Examen coproparazitologic = Frotiu din fecale coloratie Tricrom

Entamoeba hystolitica
Trophe (gr)= hranire; zoon (gr)= animal Stadiul de TROFOZOIT

Examen coproparazitologic = Frotiu din fecale muco-piosangvinolente coloratie Giemsa

Entamoeba hystolitica
Colonoscopie Abcese primare in buton de camasa

Entamoeba hystolitica
Abces primar in buton de camasa

Examen histopatologic = Sectiune tesut intestinal coloratie H&E

Entamoeba hystolitica
Abces secundar amoebian - hepatic

Entamoeba hystolitica
Abces secundar amoebian -perianal

AMIBE COMENSALE

Entamoeba coli
Stadiul de CHIST

Examen coproparazitologic = Preparat proaspat L+l din fecale + solutie Lugol

8 nuclei in planuri suprapuse

AMIBE LIBERE CONDITIONAT PATOGENE 1. 2. Naegleria fowlery Acanthamoeba sp.

Naegleria fowlery

Naegleria fowlery
Stadiul de CHIST

Naegleria fowlery
Stadiul FLAGELAT

Flagel (2)

Naegleria fowlery
Stadiul de TROFOZOIT

Recunoastere dupa nucleul cu aspect de tinta

Examen histopatologic = Sectiune tesut cerebral coloratie H&E

Acanthamoeba sp.

Acanthamoeba sp.
Stadiul de CHIST

Preparat proaspat L+l din suspensie tesut de raclaj corneean

Acanthamoeba sp.
Stadiul de TROFOZOIT

Nucleu

Preparat proaspat L+l din LCR

Acantopodii

Acanthamoeba sp.
Keratita amoebiana

Acanthamoeba sp. Abcese subcutanate granulomatoase (la imunosupresati)

Acanthamoeba sp.
Encefalita granulomatoasa amoebiana cronica (la imunosupresati)

FLAGELATE

FLAGELATE CAVITARE
1. 2. Giardia duodenalis Trichomonas vaginalis

Giardia duodenalis

Giardia duodenalis
Stadiul de CHIST

Examen coproparazitologic = Preparat proaspat L+l din fecale formate + SF

Giardia duodenalis
Stadiul de TROFOZOIT

Examen coproparazitolo gic = Frotiu fecale diareice coloratie Giemsa

Giardia duodenalis
Stadiul de TROFOZOIT

Ventuza/Disc adeziv Instalare MALABSORTII

Trichomonas vaginalis

Trichomonas vaginalis
Stadiul de TROFOZOIT

Frotiu secretie coloratie Giemsa

Trichomonas vaginalis
Coposcopie Vaginita + Leucoree FAZA INCIPIENTA

Trichomonas vaginalis
Colposcopie Strawberry cervix + coinfectie cu Candida

Trichomonas vaginalis

Testul InPouch TV pentru detectia directa si cultivarea trofozoitilor

Trichomonas vaginalis
Test rapid imunocromatografic detectie antigene

HEMOFLAGELATE
1. 2. 3. Leishmania sp. Trypanosoma brucei Trypanosoma cruzii

Leishmania sp.

Leishmania sp.
(Sandfly)

Phlebotomus sp

Lutzomia sp.

Mastigot = kinetida (gr) = flagel+ corpuscul bazal/kinetosom

Leishmania sp.
Stadiul PROMASTOGOT

Flagel liber

kinetoplast

Frotiu GLANDE SALIVARE MUSCULITA DE NISIP - coloratie GIEMSA

nucleu

Leishmania sp.
Stadiul AMASTOGOT Amastigot uninucleat

kinetoplast Frotiu aspirat medular coloratie Giemsa

Macrofag

Leishmania sp.
Buton de Orient - Forma uscata - L. tropica

Leishmania sp.
Buton Orient - Forma umeda L. major

Leishmania sp.
Forma ESPUNDIA L. brasiliensis

Leishmania sp.
Ulcerul chiclero- L. mexicana

Boala Kala-azar, febra Dum-Dum, boala neagra FAZA CRONICA

Leishmania sp.

L. donovani L. Infantum L. chagasi

Leishmanioza cutanata post kala-azar FAZA CRONICA

Leishmania sp.
Leismania donovani

Trypanosoma brucei

Trypanosoma brucei
Glossina sp. ( musca tze-tze )

Trypanosoma brucei
Stadiul EPIMASTOGOT

Membrana ondulanta scurta Frotiu din cultura dezvoltata pe mediul NNN coloratie GIEMSA

Flagel liber anterior

Nucleu

Trypanosoma brucei
Stadiul TRIPOMASTIGOT Membrana ondulanta lunga

Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA

Corpuscul bazal /Kinetosom

Kinetoplast

Nucleu

Flagel liber anterior

Trypanosoma brucei gambiense


Sleeping sickness (Boala somnului) FAZA INCIPIENTA

Semnul Winterbottom

Trypanosoma brucei
Sleeping sickness (Boala somnului) FAZA CRONICA

Meningoencefalita cronica

Trypanosoma cruzii

Trypanosoma cruzii

Triatoma sp. (Kissing bug) Reduviid bug

Trypanosoma cruzii
Stadiul TRIPOMASTIGOT

Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA

Trypanosoma cruzii
Stadiul AMASTOGOT Amastogoti uninucleati

Celula nervoasa

Aprenta tesut cardiac coloratie Giemsa

Trypanosoma cruzii
Boala Chagasi (FAZA INCIPIENTA)

Semnul Romana

Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)

Cardiomiopatie cu megalie cardiaca

Anevrism cardiac

Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)

Megacolon

SPOROZOARE

Sporozoare tisulare 1. Toxoplasma gondii

Toxoplasma gondii

Toxoplasma gondii
Stadiul de OOCHIST P.C. Sporozoiti uninucleati

Sporochisti

Examen coproparazitologic fecale PISICA

Toxoplasma gondii
Stadiul de TACHIZOIT (trofozoitul de Toxoplasma)

Frotiu ascita soarece infectat coloratie Giemsa

TACHIZOITI uninucleati

Macrofag
tachys (gr)=rapid; zoon (gr)= animal

Toxoplasma gondii
Stadiul de CHIST TISULAR cu bradizoiti Celula nervoasa

BRADIZOITI

bradys (gr)=lent; zoon (gr)= animal

Amprenta tesut cerebral coloratie Giemsa

Toxoplasma gondii Neurotoxoplasmoza (la imunosupresati)

CT

Toxoplasma gondii
Corioretinita (la imunosupresati si la nou-nascuti cu toxoplasmoza congenitala)

Toxoplasma gondii
Hidrocefalie

Sporozoare tisulare
2. Plasmodium vivax Plasmodium ovale Plasmodium malariae Plasmodium falciparum

Plasmodium sp.

Plasmodium sp.
Tantarul Anopheles sp.

Plasmodium sp.
OOCHISTI fixati pe fata externa a stomacului unui tantar infectat.

Plasmodium sp.
SPOROZOITI

Frotiu GLANDE SALIVARE TANTAR - coloratie GIEMSA

Plasmodium sp.
Amprenta tesut hepatic coloratie Giemsa

MEROZOITI INTRAHEPATOCITARI

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium vivax INEL= TROFOZOIT TANAR

Nucleu inel

Citoplasma putina, periferica

Vacuola centrala

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium vivax AMOEBA = TROFOZOIT MATUR

Citoplasma bogata, cu multe prelungiri

Nucleu amoeba

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium vivax ROZETA = SCHIZONT MATUR 16-24 merozoiti Granulatii Schuffner

Nuclei merozoiti

Corp muriform

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium vivax GAMETOCIT MASCULIN

Granulatii Schuffner

Nucleu central nedefinit

Plasmodium sp.
Plasmodium vivax GAMETOCIT FEMININ Granulatii Schuffner Frotiu SANGE PERIFERIC coloratie GIEMSA Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium ovale INEL= TROFOZOIT TANAR

Nucleu inel

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium ovale AMOEBA = TROFOZOIT MATUR

Nucleu amoeba

Plasmodium sp. Plasmodium ovale


ROZETA = SCHIZONT MATUR 8-12 merozoiti

Frotiu SANGE PERIFERIC coloratie GIEMSA Nuclei merozoiti

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium ovale

GAMETOCIT MASCULIN

Granulatii Schuffner / James

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium ovale GAMETOCIT FEMININ Granulatii Schuffner / James

Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium malarie INEL= TROFOZOIT TANAR

Nucleu inel

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium malarie AMOEBA = TROFOZOIT MATUR

Amoeba in banda Granulatii Ziemann Nucleu amoeba

Plasmodium sp.
Plasmodium malarie ROZETA = SCHIZONT MATUR 8-12 merozoiti Rozeta in margareta Examen hematologic = Picatura groasa SANGE PERIFERIC coloratie GIEMSA

Nuclei merozoiti

Granulatii Ziemann Nuclei granulocit

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium malarie GAMETOCIT MASCULIN

Granulatii Ziemann

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium malarie GAMETOCIT FEMININ

Granulatii Ziemann

Nucleu gametocit periferic, triunghiular

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium falciparum INEL= TROFOZOIT TANAR

Inele uni si bi nucleate Hematie multiparazitata cu inele

FAZA INCIPIENTA

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium falciparum AMOEBA = TROFOZOIT MATUR

FAZA COMATOASA

Plasmodium sp.
Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA Plasmodium falciparum ROZETA = SCHIZONT MATUR 8-32 merozoiti

Merozoiti uninucleati

granulatii Maurer FAZA COMATOASA

Plasmodium sp.
Plasmodium falciparum Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA GAMETOCIT MASCULIN in cosulet

Nucleu gametocit

granulatii Maurer

FAZA INCIPIENTA

Plasmodium sp. Plasmodium falciparum


Examen hematologic = Frotiu SANGE PERIFERIC coloratie GIEMSA GAMETOCIT FEMININ in cosulet

FAZA INCIPIENTA

Plasmodium falciparum Neuropaludism Malarie cerebrala

Cryptosporidium sp.

Sporozoare digestive
3. Cryptosporidium parvum Cryptosporidium hominis

Cryptosporidium sp.
Stadiul de OOCHIST Sporozoiti uninucleati (4)

Examen coproparazitologic = Frotiu fecale coloratie ZIEHL-NIELSEN modificata

Sporozoar digestiv 4. Isospora belli

Isospora belli

Isospora belli
Stadiul de OOCHIST Examen coproparazitologic Qochist matur Sporozoiti uninucleati Qochist imatur

Sporochist

Preparat proaspat L+ l din fecale cu SF

Frotiu fecale coloratie ZIEHL-NIELSEN modificata

Sporozoar - Transfug
5. Pneumocystis carinii Pneumocystis jiroveci

Pneumocystis carinii Pneumocystis jiroveci


Cale aerogena

Pneumocystis carinii

TROFOZOITI (nuclei trofozoiti in nor cu puncte)

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii

TROFOZOITI CHISTI cu (8) corpusculii colorati

Frotiu LBA coloratie GIEMSA.

Pneumocystis carinii
CHISTI cu perete celular colorat

Frotiu LBA coloratie Albastru de Toluidina.

Pneumocystis carinii

RX- plamani in geam mat