Streptozotocin (STZ; N-nitro derivative of glucosamine) is a naturally occurring, broadspectrum antibiotic and cyto-toxic chemical that is particularly toxic

to the pancreatic, insulinproducing beta cells in mammals [6,17,18,19]. Induction of experimental diabetes in the rat using streptozotocin is very convenient and simple to use [2,10,19]. Streptozotocin injection leads to the degeneration of the Langerhans islets beta cells [9,15,19]. Clinically, symptoms of diabetes are clearly seen in rats within 2-4 days following single intravenous or intraperitoneal injection of 60mg/kg STZ.

Rats were fasted for 12-h before diabetes was induced using STZ. NR and SDR received a single intraperitoneal injection of 150mg/kg of STZ (Sigma, St. Louis, MO, USA). STZ was freshly dissolved in 0.05 M citrate buffer, pH 4.5. For the i.p. injection of STZ, the rat was held in one hand in dorsal position, the injection site was swabbed using povidone- iodine solution and the designated amount of STZ was injected in the caudal abdominal cavity using sterile 25g needle.

2 g of crystalline powdered alloxan purchased from NAFCO Nigeria Limited was taken and dissolved in 50 mls of distilled water to yield a concentration of 40 mg/ml. 150 mg/kg body weight of alloxan was administered intraperitoneally (18) to 64 of the animals, after an overnight fast (access to only water) of 12 hours to make them more susceptible to developing diabetes (19, 20)

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