Documente Academic
Documente Profesional
Documente Cultură
COMPONENTS
http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html
http://www.microscopyu.com/articles/polarized/polarizedintro.html
PHASE CONTRAST
*Phase Contrast Microscope invented in 1932 by Fritz Zernike. *Phase contrast is used to look at materials that are transparent, such as asbestos or biological samples. * Can be used for live specimens.
http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html
*Light traveling through transparent objects travels slower than in air causing small differences in the wavelength speed. Light waves of different speeds are brought together, causing destructive interference, allowing small and low contrast samples to be viewed.
*The light passes through a polarizer and an analyzer and interacts with the light refracting form the specimen to form an image.
ENPH MICROSCOPE
SAFETY
*Caution needs to be taken when working with silica, asbestos or other samples. *Work in the hood to prevent breathing in airborne particles when creating the slides. *Wear proper gloves to prevent contamination of the sample and protect your hand. *Goggles and/or a respirator may be needed when handling dangerous substances or preparing slides. *Be careful when handing slides to prevent breakage.
SAFETY
*Dont work for long periods leaning over the microscope to prevent eye strain, cramps and muscle strain. Take breaks and focus on a distant object every 10 to 30 minutes, stretch at least every hour. *Use foam pads to support arms and prevent leaning directly on hard surfaces. *Minimize glare and reflective surfaces in the work area to prevent eye fatigue. *Try to set the microscope to a height so that your feet are flat on the ground (or on a stool) and you do not need to lean over the microscope to see in the oculars. *Use a chair with back support. *Keep room humidity between 30% and 50% to prevent dry eyes. *Spread microscope use between multiple people.
SAFETY
Proper Egonomics
https://cfo.asu.edu/ergonomics-lab-microscopy
SOURCES
Murphy, D., Oldfield, R., Schwartz, S., Davidson, M., Introduction to Microscope, http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.h tml 2. http://www.olympusmicro.com/primer/techniques/polarized/polarizedh ome.html 3. http://www.osha.gov/dts/sltc/methods/inorganic/id191/id191.html 4. http://www.microscopyu.com/articles/polarized/polarizedintro.html 5. http://www.lonestar.edu/16762.htm
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