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Universal ProbeLibrary Result from ProbeFinder version 2.49 for Human

Report downloaded: Sun Apr 14 23:38:33 CEST 2013
This reports contains results for: NM_001675.2, NM_182810.1

ProbeFinder has designed real-time PCR assays that are common for the 2 input sequences.

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1. Assay summary
Input Probe ID Primer Length Position Tm %GC Sequence ggtcagtccctccaacaa ca ctatacccaacagggcat cc

NM_001675.2

#88 cat. no. 04689135001

Left Right

20 20

1083 1102 1170 1189

60 59

55 55

Amplicon (107 nt)

ggtcagtccctccaacaacagcaaggaggatgccttctccgggacagattggat gttggagaaaatggatttgaaggagttcgacttggatgccctgttgggtatag

NM_182810.1

#88 cat. no. 04689135001

Left Right

20 20

481 - 500 568 - 587

60 59

55 55

ggtcagtccctccaacaa ca ctatacccaacagggcat cc

Amplicon (107 nt)

ggtcagtccctccaacaacagcaaggaggatgccttctccgggacagattggat gttggagaaaatggatttgaaggagttcgacttggatgccctgttgggtatag

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2. NM_001675.2
ProbeFinder has designed an optimal real-time PCR assay for: NM_001675.2 Homo sapiens activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4), transcript variant 1, mRNA Use probe #88 (cat. no. 04689135001) Primer Left Right Length 20 20 Position 1083 - 1102 1170 - 1189 Tm 60 59 %GC 55 55 Sequence
ggtcagtccctccaacaaca ctatacccaacagggcatcc

Amplicon (107 nt)

ggtcagtccctccaacaacagcaaggaggatgccttctccgggacagattggatgttggagaaaatggatttgaaggagttcgacttgg atgccctgttgggtatag

The search was for intron spanning assays. This assay has: All criteria met.

Length of intron(s) spanned by this assay: 100 nt

Input sequence:
>NM_001675.2 tttctactttgcccgcccacagatgtagttttctctgcgcgtgtgcgttttccctcctcc ccgccctcagggtccacggccaccatggcgtattaggggcagcagtgcctgcggcagcat tggcctttgcagcggcggcagcagcaccaggctctgcagcggcaacccccagcggcttaa gccatggcgtgagtaccggggcgggtcgtccagctgtgctcctggggccggcgcgggttt tggattggtggggtgcggcctggggccagggcggtgccgccaagggggaagcgatttaac gagcgcccgggacgcgtggtctttgcttgggtgtccccgagacgctcgcgtgcctgggat cgggaaagcgtagtcgggtgcccggactgcttccccaggagccctacagccctcggaccc cgagccccgcaagggtcccaggggtcttggctgttgccccacgaaacgtggcaggaacca agatggcggcggcagggcggcggcgcgggcgtgagtcaagggcgggcggtgggcggggcg cggccgccctggccgtatttggacgtggggacggagcgctttcctcttggcggccggtgg aagaatcccctggtctccgtgagcgtccattttgtggaacctgagttgcaagcagggagg ggcaaatacaactgccctgttcccgattctctagatggccgatctagagaagtcccgcct cataagtggaaggatgaaattctcagaacagctaacctctaatgggagttggcttctgat tctcattcaggcttctcacggcattcagcagcagcgttgctgtaaccgacaaagacacct tcgaattaagcacattcctcgattccagcaaagcaccgcaacatgaccgaaatgagcttc ctgagcagcgaggtgttggtgggggacttgatgtcccccttcgaccagtcgggtttgggg gctgaagaaagcctaggtctcttagatgattacctggaggtggccaagcacttcaaacct catgggttctccagcgacaaggctaaggcgggctcctccgaatggctggctgtggatggg ttggtcagtccctccaacaacagcaaggaggatgccttctccgggacagattggatgttg gagaaaatggatttgaaggagttcgacttggatgccctgttgggtatagatgacctggaa accatgccagatgaccttctgaccacgttggatgacacttgtgatctctttgccccccta gtccaggagactaataagcagcccccccagacggtgaacccaattggccatctcccagaa agtttaacaaaacccgaccaggttgcccccttcaccttcttacaacctcttcccctttcc

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ccaggggtcctgtcctccactccagatcattcctttagtttagagctgggcagtgaagtg gatatcactgaaggagataggaagccagactacactgcttacgttgccatgatccctcag tgcataaaggaggaagacaccccttcagataatgatagtggcatctgtatgagcccagag tcctatctggggtctcctcagcacagcccctctaccaggggctctccaaataggagcctc ccatctccaggtgttctctgtgggtctgcccgtcccaaaccttacgatcctcctggagag aagatggtagcagcaaaagtaaagggtgagaaactggataagaagctgaaaaaaatggag caaaacaagacagcagccactaggtaccgccagaagaagagggcggagcaggaggctctt actggtgagtgcaaagagctggaaaagaagaacgaggctctaaaagagagggcggattcc ctggccaaggagatccagtacctgaaagatttgatagaagaggtccgcaaggcaaggggg aagaaaagggtcccctagttgaggatagtcaggagcgtcaatgtgcttgtacatagagtg ctgtagctgtgtgttccaataaattattttgtagggaaagta

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3. NM_182810.1
ProbeFinder has designed an optimal real-time PCR assay for: NM_182810.1 Homo sapiens activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4), transcript variant 2, mRNA Use probe #88 (cat. no. 04689135001) Primer Left Right Length 20 20 Position 481 - 500 568 - 587 Tm 60 59 %GC 55 55 Sequence
ggtcagtccctccaacaaca ctatacccaacagggcatcc

Amplicon (107 nt)

ggtcagtccctccaacaacagcaaggaggatgccttctccgggacagattggatgttggagaaaatggatttgaaggagttcgacttgg atgccctgttgggtatag

The search was for intron spanning assays. This assay has: All criteria met.

Length of intron(s) spanned by this assay: 100 nt

Input sequence:
>NM_182810.1 tttctactttgcccgcccacagatgtagttttctctgcgcgtgtgcgttttccctcctcc ccgccctcagggtccacggccaccatggcgtattaggggcagcagtgcctgcggcagcat tggcctttgcagcggcggcagcagcaccaggctctgcagcggcaacccccagcggcttaa gccatggcgcttctcacggcattcagcagcagcgttgctgtaaccgacaaagacaccttc gaattaagcacattcctcgattccagcaaagcaccgcaacatgaccgaaatgagcttcct gagcagcgaggtgttggtgggggacttgatgtcccccttcgaccagtcgggtttgggggc tgaagaaagcctaggtctcttagatgattacctggaggtggccaagcacttcaaacctca tgggttctccagcgacaaggctaaggcgggctcctccgaatggctggctgtggatgggtt ggtcagtccctccaacaacagcaaggaggatgccttctccgggacagattggatgttgga gaaaatggatttgaaggagttcgacttggatgccctgttgggtatagatgacctggaaac catgccagatgaccttctgaccacgttggatgacacttgtgatctctttgcccccctagt ccaggagactaataagcagcccccccagacggtgaacccaattggccatctcccagaaag tttaacaaaacccgaccaggttgcccccttcaccttcttacaacctcttcccctttcccc aggggtcctgtcctccactccagatcattcctttagtttagagctgggcagtgaagtgga tatcactgaaggagataggaagccagactacactgcttacgttgccatgatccctcagtg cataaaggaggaagacaccccttcagataatgatagtggcatctgtatgagcccagagtc ctatctggggtctcctcagcacagcccctctaccaggggctctccaaataggagcctccc atctccaggtgttctctgtgggtctgcccgtcccaaaccttacgatcctcctggagagaa gatggtagcagcaaaagtaaagggtgagaaactggataagaagctgaaaaaaatggagca aaacaagacagcagccactaggtaccgccagaagaagagggcggagcaggaggctcttac tggtgagtgcaaagagctggaaaagaagaacgaggctctaaaagagagggcggattccct ggccaaggagatccagtacctgaaagatttgatagaagaggtccgcaaggcaagggggaa

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gaaaagggtcccctagttgaggatagtcaggagcgtcaatgtgcttgtacatagagtgct gtagctgtgtgttccaataaattattttgtagggaaagta