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  • Use of HPTLC, HPLC, and Densitometry For Qualitative Separation of Indole Alkaloids From Rauvolfia Serpent in A Roots (M. M. Gupta, Alpana Srivastava Arvind K. Tripathi, Himanshu Misra, R. K. Verma)

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    Himanshu Misra
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    High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids.

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    Use of HPTLC, HPLC, And Densitometry for Qualitative Separation of Indole Alkaloids From Rauvolfia Serpent in A Roots [M. M. Gupta, Alpana Srivastava Arvind K. Tripathi, Himanshu Misra, R. K. Verma]

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    High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids.

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    863 vizualizări2 pagini

    Use of HPTLC, HPLC, and Densitometry For Qualitative Separation of Indole Alkaloids From Rauvolfia Serpent in A Roots (M. M. Gupta, Alpana Srivastava Arvind K. Tripathi, Himanshu Misra, R. K. Verma)

    Încărcat de

    Himanshu Misra
    High-performance thin-layer chromatography (HPTLC) has been used for normal-phase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-dimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids.

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    Abstract

    High-performance thin-layer chromatography (HPTLC) has been used for normal-


    phase separation of the components of hexane, chloroform, methanol, and water
    extracts of Rauvolfia serpentina roots. Computerized densitometry was used for two-
    dimensional spectrographic image analysis of the HPTLC plates. High performance
    liquid chromatography (HPLC) was also used for reversed-phase separation of
    these extracts. Different chromatograms of R. serpentina root extracts, obtained by
    use of these techniques, revealed the presence of three marker indole alkaloids,
    ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in
    most efficient extraction of these three alkaloids. The results also showed that
    defatting with hexane may result in loss of the alkaloids.

    Keywords

    HPTLC, HPLC, Densitometry, Rauvolfia serpentina, Alkaloids

    References

    H.J. Pfeifer, D.J. Greenblatt , and J. Koch-Weser , Am. J. Med. Sci. 271 (1976) 269–
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