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Universitatea de Medicina si Farmacie Victor Babes Timisoara

Rspopescu Diana, seria B, gr.10 Tomit Georgiana, seria A, gr. 2

Efectul nanoparticulelor de aur asociate cu unde electromagnetice asupra celulelor maligne

Scopul studiului
Gasirea unei metode pt distrugerea celulelor maligne Caracteristici : - neinvaziva - viabila pt distrugerea celulelor maligne - specificitate mare - toxicitate redusa

Unde electromagnetice si nanoparticulele de aur


Celelele maligne pot fi distruse termic datorita undelor electromagnetice amplificate din mediu.

De ce nanoparticule de aur?

Ipoteza de nul a studiului:

1. GNPs-citotoxice 2. Celulele se dezvolta letal 3. Caldura produce injurii

Metode

Laborator: GNPs 5nm-metode biochimice, filtrare (filtru de 0.22u). In vitro 4 serii celulare maligne: Hep3B, Panc-1. Serii control doar RF/serii RF+particule GNPs. Studiu case contol/ test t pereche pt.:

Diferite concentratii de GNPs-1, 10, 67 ul/l-Expunere la RF: 1, 2, 5 min, Analiza ME.

Rezultate-citotoxicitatea

Viabilitate celule normale (fct. de C% GNPs): Hep3B: 1uM/l => 1005%


10uM/l =>986% 67uM/l => 86 8%

Panc-1:

1uM/l => 9810% 10uM/l =>9011% 67uM/l => 81 9%

Rezultate-celule maligne distruse

Cel. maligne distruse -doar RF: 1 min- <20%


2min- <27%

-Pt GNPs(67uM/l)+RF-1,2,5 min~ 98%

Pt. 67um/l GNP+RF / RF => p=0.001

Valoarea P

GNPs + RF

Discutie

RFA conventional Studii anterioare

GNPs-avantaje
De urmarit pe viitor

Discutie

RFA conventional Studii anterioare

GNPs-avantaje
De urmarit pe viitor

Discutie

RFA conventional Studii anterioare

GNPs-avantaje
De urmarit pe viitor

Discutie

RFA conventional Studii anterioare

GNPs-avantaje
De urmarit pe viitor

Concluzii

GNPs nu prezinta efect citotoxic sau antiproliferativ pentru celulele umane.


GNPs asociate cu expunerea RF determina distrugerea termica celulelor maligne din tumorile gastrointestinale.

Efectul GNPs+RF

Referinte

1. Bilchik AJ, Wood TF, Allegra D, TsioulRamming KP, Morton DL: Cryosurgical ablation and radiofrequency ablation for unresectable hepatic malignant neoplasms: a proposed algorithm. 2. Bleicher RJ, Allegra, Bilchik AJ: Radiofrequency ablation in 447 complex unresectable liver tumors: lessons learned. 3. Curley SA, Marra P, Beaty Early and late complications after radiofrequency ablation of malignant liver tumors in 608 patients. 4. Haemmerich D, Laeseke PF: Thermal tumour ablation: devices, clinical applications and future directions. 5. Gannon CJ, Curley SA: The role of focal liver ablation in the treatment of unresectable primary and secondary malignant liver tumors. 6. Raut CP, Izzo F, Marra P, Ellis LM, Vauthey JN, Cremona F, Curley SA: Significant long-term survival after radiofrequency ablation of unresectable hepatocellular carcinoma in patients with cirrhosis.

Va multumim!