ALLERGY MANAGEMENT

14th June 2013

MANAGEMENT OF ALLERGIC DISEASE IN COMPANION ANIMALS

Dr. Brusati
Dr. Tambone

ALLERGY TESTING AND TREATMENTS

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AGROLABO has been involved in Allergy management for more than 20 years, offering to vets:
HIGHLY

KITS

SPECIFIC INTRADERMAL TEST IN-VITRO

TECHNICALLY-ADVANCED PERSONALISED

SERUM TESTING SERVICES

PATIENT-SPECIFIC IMMUNOTHERAPY TREATMENTS

ALLERGY TESTING
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PRINCIPAL METHODS
INTRADERMAL TESTING

ELISA TESTING

ALLERGY TESTS MUST NOT TO BE CONSIDERED AS DIAGNOSTIC TESTS FOR ATOPY

DIAGNOSIS HAS TO BASED ON THE COMBINATION OF DIFFERENT PARAMETERS: PATIENT HISTORY, CLINICAL SIGNS AND DIFFERENTIAL DIAGNOSES

ALLERGY TESTING
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PRINCIPAL METHODS
INTRADERMAL TESTING

ELISA TESTING

THE REAL UTILITY OF ALLERGY TESTS IS TO IDENTIFY AND SELECT THE ALLERGENS TO WHICH THE ANIMAL IS SENTISITVE AND PREPARE THE PERSONALISED IMMUNOTHERAPY

ALLERGY TESTING
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PRINCIPAL METHODS
INTRADERMAL TESTING

ELISA TESTING

HOWEVER PERFORMING BOTH TESTS GIVES THE BEST INFORMATION ON THE PATIENTS SENSITIVITY
(DEBOER, 2012)

NO METHOD HAS BEEN CONCLUSIVELY SHOWN TO BE THE BEST,

INTRADERMAL TESTING
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INTRADERMAL TESTING DETECTS NOT ONLY THE PRESENCE OF SPECIFIC IgE ANTIBODIES, BUT ALSO THE FUNCTIONAL ABILITY FOR CUTANEOUS MAST CELLS TO DEGRANULATE UPON EXPOSURE TO ALLERGEN EXTRACTS

INTRADERMAL TESTING
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and storage are key factors Corticosteroids adversely affect reactivity of the skin and it is necessary to discontinue the administration for weeks The majority of dermatologists prefer to use sedation for testing The animal must be shaved No standardized criteria are used for interpretation of reactions

Allergen quality, dilution

INTRADERMAL TESTING is the method of choice for many specialists and is particularly appreciated because it is relatively free from false positive reactions (high specifity)

HOWEVER

AGROLABO AGROSKIN TEST KITS

IN-VIVO TESTS TO IDENTIFY SPECIFIC ALLERGENS IN CATS, DOGS AND HORSES
DIFFERENT KITS ARE AVAILABLE:
=diluted allergens to perform Agroskin RTU 20

20 SKIN TESTS

= concentrated allergen extracts to perform

Agroskin CT 100 or Agroskin CT 200

100 or 200 SKIN TESTS

AGROLABO AGROSKIN TEST KITS

AGROSKIN RTU 20, AGROSKIN CT 100, AGROSKIN CT 200:

TAILOR-MADE KITS WITH PERSONALISED SELECTION OF

ALLERGEN EXTRACTS

KITS MANUFACTURED UPON REQUEST IN ORDER TO

GUARANTEE MAXIMUM SHELF-LIFE

HIGHLY SPECIFIC AND RELIABLE RESULTS COST EFFECTIVE

ALLERGENS AVAILABLE FOR AGROSKIN TEST KITS

GRASS POLLEN
ANTHOXANTUM ODORATUM (SWEET VERNAL) CYNODON DACTYLON (BERMUDA GRASS) DACTYLIS GLOMERATA (ORCHARD GRASS) FESTUCA ELATIOR (MEADOW FESCUE)

WEED POLLEN
AMBROSIA MIX (RAGWEED SP.) ARTEMISIA VULGARIS (COMMON MUGWORT) CHENOPODIUM ALBUM (LAMB'S QUARTER) PLANTAGO LANCEOLATA (ENGLISH PLANTAIN)

TREE POLLEN
ACER SPP. (MAPLE) ALNUS SPP. (ALDER) BETULA SPP. (BIRCH) CORYLUS SPP. (HAZEL)

HOLCUS LANATUS (VELVET)

LOLIUM PERENNE (PERENNIAL RYEGRASS) PHLEUM PRATENSE (TIMOTHY GRASS) POA PRATENSIS (KENTUCKY BLUE GRASS) SECALE CEREALE (CULTIVATED RYE)

RUMEX ACETOSELLA (SHEEP SORREL)

TARAXACUM OFFICINALE DANDELION) URTICA DIOICA (STINGING NETTLE)

FAGUS SPP. (BEECH)

FRAXINUS SPP. (ASH) OLEA EUROPEA (OLIVE) PLATANUS OCCIDENTALIS (WESTERN CAMORE) POPULUS NIGRA (BLACK POPULAR) QUERCUS SPP. (OAK) SALIX SPP. (WILLOW)

ALLERGENS AVAILABLE FOR AGROSKIN TEST KITS

MITES
ACARUS SIRO (FLOUR MITE)
DERMATOPHAGOIDES FARINAE (DUST MITE)

FUNGI AND YEAST

ALTERNARIA ALTERNATA (FUNGUS)
ASPERGILLUS SPP. (FUNGUS) CLADOSPORIDIUM HERBRUM (FUNGUS) MALASSEZIA PACHIDERMATIS (YEAST)

OTHERS
CAT EPITHELIA
FEATHER MIX HUMAN DANDER

DERMATOPHAGOIDES PTERONYSSINUS (DUST MITE)

LEPIDOGLYPHUS DESTRUCTOR (FORAGE MITE)

TYROPHAGUS PUTRESCENTIAE (FOOD MITE)

PENICILLIUM NOTATUM (MOULD)

FEATHER

SEROLOGICAL TESTING ELISA SEROLOGY TEST METHODS

THE ELISA SEROLOGY TEST METHOD MEASURES THE AMOUNT OF ALLERGEN SPECIFIC IgE ANTIBODIES IN SERUM SAMPLES

If steroid or antihistamine treatments are not prolonged and not at high dosages, they do not need to be suspended No shaving or sedation required Ideal for patients with unhealthy skin

HOWEVER

Not standardised results are offered The quality of detection reagents is a key factor (detection of only IgE antibodies and not IgG or IgM)

IgE DETECTION ANTIBODY (CONJUGATE)

POLYCLONAL ANTIBODY
CROSS-REACTS EXTENSIVELY WITH IgG: LOW SPECIFICITY, HIGH SENSITIVITY

MONOCLONAL ANTIBODY
DOES NOT CROSS-REACT WITH IgG: HIGH SPECIFICITY, LOWER SENSITIVITY

HUMAN HIGH-AFFINITY IgE RECEPTOR

UNIQUE BINDING SITE FOR THE FRAGMENT Fc OF IgE: HIGH SPECIFICITY, LOWER SENSITIVITY

GREER mac (Monoclonal Antibodies Cocktail) ELISA

IgE Allergen Solid Phase

GREER mac (Monoclonal Antibodies Cocktail) ELISA

Anti-IgE Biotin

IgE Allergen

Fragment FC

Solid Phase

AGROLABO SEROLOGICAL TESTING

AGROLABO OFFERS AN IgE ALLERGEN-SPECIFIC TEST, WHICH ALLOWS FOR THE DETECTION OF ALLERGEN SPECIFIC IgE LEVELS TO INDIVIDUAL ALLERGENS

WIDE RANGE OF ALLERGENS IN DIFFERENT PANELS

EACH PANEL CONTAINS FROM 6 TO 18 INDIVIDUAL ALLERGENS, WHICH ARE
SPECIFIC FOR CATS, DOGS AND HORSES

VERY VALUABLE TOOL TO DETERMINE WHICH ALLERGENS SHOULD BE INCLUDED IN

the ALLERGEN-SPECIFIC IMMUNOTHERAPY TREATMENT

SEMI-QUANTITATIVE RESULTS expressed as Elisa Allergy Units

AGROLABO mac ELISA SEROLOGICAL PANELS

ALLERGEN-SPECIFIC IgE TESTING FOR DOGS AND CATS

PRESCREENING PANEL SEASONAL (POLLEN) PANEL PERENNIAL (INDOOR) PANEL FOOD PANEL

ALLERGEN-SPECIFIC IgE TESTING FOR HORSES

PRESCREENING PANEL SEASONAL (POLLEN) PANEL PERENNIAL (INDOOR) PANEL FOOD PANEL

PRESCREENING PANEL ALLERGEN GROUPS

POLLEN WELL
AMBROSIA MIX, BETULA SP., CYNODON DACTYLON, LOLIUM PERENNE, OLEA AEUROPEA, PLANTAGO LANCEOLATA, PARIETARIA OFFICINALIS, POA PRATENSIS, QUERCUS SPP.

FUNGI WELL
ALTERNARIA ALTERNATA, ASPERGILLUS SP., CLADOSPORIUM HERBARUM, PENICILLIUM NOTATUM

FLEA WELL
CTENOCEPHALIDES FELIS, CTENOCEPHALIDES CANIS

MITE WELL
ACARUS SIRO, DERMATOPHAGOIDES FARINAE, DERMATOPHAGOIDES PTERONYSSINUS, TYROPHAGUS PUTRESCENTIAE

SPECIFIC ALLERGEN PANEL FOR CATS AND DOGS

SEASONAL (POLLEN) PANEL
GRASSES
POA PRATENSIS, DACTYLIS GLOMERATA, LOLIUM PERENNE, FESTUCA ELATIOR (MIX 1 GRASSES) BROMUS INERMIS, CYNODON DACTILON (MIX 2 GRASSES)

WEEDS
ARTEMISIA VULGARIS (COMMON MUGWORT)

TREES
CUPRESSUS SEMPERVIRENS. (CYPRESS)

CHENOPODIUM ALBUM (LAMB'S QUARTER) MIX AMBROSIA (RAGWEED) PARIETARIA OFFICINALIS (PELLITTORY) PLANTAGO LANCEOLATA (ENGLISH PLANTAIN)

BETULA PENDULA (BIRCH) OLEA EUROPEA (OLIVE) POPULUS MIX (POPLAR) QUERCUS SP. (OAK)

SPECIFIC ALLERGEN PANELS FOR CATS AND DOGS

PERENNIAL (INDOOR) PANEL
MITES
ACARUS SIRO (FLOUR MITE)

FOOD PANEL
FOOD
BEEF, CHICKEN, AND TURKEY, DUCK LAMB, RABBIT FISH MIX EGGS MILK

FUNGI / YEAST
ALTERNARIA ALTERNATA (FUNGI) ASPERGILLUS FUMIGATUS (FUNGI) CLADOSPORIUM HERBARUM (MOULD) MALASSEZIA PACHIDERMATIS (YEAST) PENICILIUM NOTATUM (MOULD)

FLEAS
CTENOCEPHALIDES FELIS, CANIS (FLEA)

DERMATOPHAGOIDES FARINAE (DUST MITE) DERMATOPHAGOIDES PTERONYSSINUS (DUST MITE) GLYCIPHAGUS DOMESTICUS (GRAIN MITE) LEPIDOGLYPHUS DESTRUCTOR (FORAGE MITE) TYROPHAGUS PUTRESCENTIAE (FOOD MITE)

CORN AND WHEAT POTATO, SOYABEAN RICE

SPECIFIC ALLERGEN PANEL FOR HORSES

SEASONAL PANEL
GRASSES
POA PRATENSIS, DACTYLIS GLOMERATA, LOLIUM PERENNE, FESTUCA ELATIOR (MIX 1 GRASSES) BROMUS INERMIS, CYNODON DACTILON (MIX 2 GRASSES) PHLEUM PRATENSE (GRASS) TRITICUM, AVENA, ZEA MAYS, HORDEUM, SECALE (CEREAL)

WEEDS
ARTEMISIA VULGARIS (COMMON MUGWORT)

TREES
CUPRESSUS SEMPERVIRENS (CYPRESS)

BRASSICA NAPUS (RAPE) CHENOPODIUM ALBUM (LAMBS QUARTER) PARIETARIA OFFICINALIS (PELLITTORY) PLANTAGO LANCEOLATA (ENGLISH PLANTAIN) RUMEX ACETOSELLA (SHEEP SORREL)

BETULA PENDULA (BIRCH) FAGUS SP. (BEECH) POPULUS MIX (POPLAR) FRAXINUS SP. (ASH) SALIX SP. (WILLOW) OLEA EUROPEA (OLIVE)

SPECIFIC ALLERGEN PANELS FOR HORSES

PERENNIAL (INDOOR) PANEL
MITES
ACARUS SIRO (FLOUR MITE) DERMATOPHAGOIDES FARINAE (DUST MITE) DERMATOPHAGOIDES PTERONYSSINUS (DUST MITE) GLYCIPHAGUS DOMESTICUS (GRAIN MITE) LEPIDOGLYPHUS DESTRUCTOR (FORAGE MITE) TYROPHAGUS PUTRESCENTIAE (FOOD MITE)

FOOD PANEL
FOOD
MILK SOYABEAN CORN, BARLEY, OAT WHEAT APPLE MOLASSA ALFAALFA CARROTS, POTATO MALT

FUNGI / YEAST
ALTERNARIA ALTERNATA (FUNGI) ASPERGILLUS FUMIGATUS (FUNGI) USTILAGO SP. (FUNGI) RHIZOPUS NIGRICANS (FUNGI) CLADOSPORIUM HERBARUM (MOULD) PENICILIUM NOTATUM (MOULD)

INSECTS
CHRYSOPS SP. (DEER FLY) CULEX (MOSQUITO) TABANUS (HORSE FLY) CULICOIDES (MIDGE) BLATELLA GERMANICA (GERMAN COCKROACH) MUSCA DOMESTICA (HOUSE FLY)

COMPARISON BETWEEN INTRADERMAL AND ELISA TECHNIQUE

INTRADERMAL TESTING ELISA TESTING

Invasive system (shaving and sedation can be required)

It cannot be performed in the case of skin diseases Personalised selection of the allergens Suspension of the corticosteroid and/or antihistamine treatments for a long period

Simple blood sample

Simple blood sample

Preset panels

Suspension of corticosteroid
and/or antihistamine treatments is not required

Possibility of anaphylactic shock

No possibility

HOW CAN I REQUEST TESTING SERVICES FOR CATS, DOGS AND HORSES?

FILL IN THE TESTING ORDER FORM AND THE ANAMNESTIC FORM COLLECT THE SERUM SAMPLE LABEL THE TEST TUBE PLACE THE COLLECTION TUBE, TOGETHER WITH THE
ANAMNESTIC and the ORDER FORMS, IN THE SHIPPING ENVELOPE

SUBMIT TO AGROLABO

FAST AND QUALITY TESTING SERVICES

AGROLABO PROVIDES FOC:

- VIALS FOR COLLECTING THE SAMPLE - ENVELOPE FOR SHIPPING THE SAMPLE

TEST RESULTS ARE AVAILABLE WITHIN 48 HOURS

OF RECEIVING SAMPLE BY FAX, EMAIL OR POST

THE RECORD OF EACH PATIENT ARE FILED IN THE COMPUTER AGROLABO OFFERS TECHNICAL ASSISTANCE
AND COMMERCIAL ADVICE

ALLERGEN-SPECIFIC IMMUNOTHERAPY TREATMENT

THE ONLY TREATMENT THAT CAN DIRECTLY REVERSE PART OF THE PATHOGENESIS OF THE DISEASE
(DJ. DEBOER 2012)

ALLERGEN-SPECIFIC IMMUNOTHERAPY TREATMENT

Accurate testing is important to identify the offending allergens. Once they have been identified they are included in the IMMUNOTHERAPY. The IMMUNOTHERAPY TREATMENT is based on the subcutaneous injection of a gradually increasing amount of the allergen extracts to which the patient is sensitive

ALLERGEN-SPECIFIC IMMUNOTHERAPY TREATMENTS

Specific formulated immunotherapy treatment sets
for cats, dogs, and horses (it is advisable to include no more than 10 allergens) immunotherapy treatment solution depending on weight, number of allergens, administration timing vials for dogs and horses are prepared with a 9ml volume of solution is included with each treatment set

Each treatment set contains between 1 and 4 vials of

Vials for cats contain 5ml of solution;

An individual dosage schedule for each patient

ALLERGEN-SPECIFIC IMMUNOTHERAPY TREATMENTS

Side effects include localized itch at the injection
site and transient worsening of symptoms during the first days of treatment

Response to immunotherapy is 60 - 75% good to

excellent in 6 -12 months of treatment

Allergen-specific immunotherapy is

considered to be lifelong. It takes several months or more to see the response and nearly no adverse effect occurs in the great majority of animals

MARKETING MATERIALS AND SUPPLIES

AGROLABO PROVIDES AN ASSORTMENT OF FREE MARKETING MATERIALS AND SALES SUPPORT TO ASSIST IN PROMOTING ALLERGY MANAGEMENT IN THE PRACTICE

PATIENT HISTORY AND ORDER FORMS

FULL-COLOUR POSTER

LEAFLETS FOR VETS AND PET OWNERS

SPECIMEN SHIPMENT KIT

WHY SHOUD YOU USE AGROLABO?

COMPLETE SOLUTIONS

FAST SERVICE
ACCURACY OF TESTING PERSONALIZED IMMUNOTHERAPY CONSULTING ALWAYS AVAILABLE LOW PRICES

THANK YOU!
THANK YOU FOR YOUR ATTENTION