Solutions for Chromatography Exercises

1. A main cause of band broadening is diffusion of the component from a region of

high concentration to a region of lower concentration.
2. Plate height (H) is a measure of how efficient a chromatography column is in
separating components. It is defined as
where is the standard deviation, in units of length along the column, of the
analyte concentration profile in its band, and L is the length of the column. Plate
height is calculate from the number of plates associated with the column by H = L
/ N. A smaller plate height indicates that there is less band broadening (smaller
) as a component moves through the column and is more desirable than a
larger plate height.
3.
4.
Since R
s
is slightly larger than 1.5 the peaks have baseline resolution and do not
overlap significantly.