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The Effects of Menadione Alone or Combined with Ionizing Radiation on Human Hepatoma Cells

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"GHI5JKL,MINKOPQ RSTU(journal):
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V(vol.):
vol.5

R(issue.):
Issue. 2

WX(year.):
1998

YZ:
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Abstract:
Purpose: To study the killing effects of menadione alone or combined with ionizing radiation on human hepatoma Hep3B cell. Materials and Methods: log-phase Hep3B cells were treated with menadione, ionizing radiation or the combination of both agents. Three protocols for combined treatments: first, cells were pre-treated with 35 ?M menadione for 3 hours then irradiated; second, cells were irradiated followed by the menadione treatment; third, cells were concurrently treated with both agents. Results: The menadione inhibited the Hep3B tumor cells growth, the IC50 was around 60 M. The intracellular reduced glutathione was significantly decreased after menadione treatment for 3 hours. After combined treatment most surviving fractions were less than 15%. Conclusions: Both 'pre-irradiation followed by menadione' and 'concurrent treatment' have synergistic effect. However, 'pre-treatment of menadione followed by irradiation' only has the addition effect. This finding may be useful for clinical application of menadione when combined with ionizing radiation to treat cancers.