Microscope Skills Worksheet 1. ". $. . *. ,. -. .. /. 10. 11. 1". 1$. 1 .

Year 11 Biology

How does altering the iris diaphragm work to affect the light. How is this similar to how the iris in your eye works? ( ! What happens to the appearance of an air #u##le when you focus up and down? Why? ($! %f you cut out a letter &e' and made a wet mount of this and e(amined it under a microscope what would it look like? Why? ("! What does the word &)arfocal' mean when applied to a microscope? ("!

%f you mo+e the slide that you are looking at left and down what way does the image mo+e? Why? ("! Why must you #e careful when using the re+ol+ing nose piece? Why? ("!

What happens to the le+el of illumination when you change to a higher power? How can you compensate for this? ("! How do you calculate the total magnification setting of your microscope? What is the ma(imum and what is the minimum power of your microscope? ($! What happens to the resolution of your microscope as the magnification is increased? ("! When focusing on o#1ects under the microscope why should you focus #y mo+ing the lens away from the slide.? ("! What unit of measure do we use for microscope work? 2(plain why this is #etter than using millimeters? ( ! What is &the working distance'? When is it the greatest and when is it the least? ($! Why are stains sometimes used when preparing cell tissue for e(amination with the microscope? ($! 2(plain how the si3e of cells4 length and width is estimated at 00( magnification. 5sing a diagram(s! e(plain how to prepare wet mount of a specimen4 (*!

1*. 1,.

Write a paragraph to e(plain to a new student the #est way to go a#out seeing a good image of a slide of an onion tissue you had prepared at 00( magnification. (*!