How to make curcumin stock solution? I usually dissolve 0.02g extract in 1000 microlit pure DMSO as stock solution.

For dilution, I add 10 microlit o t!e stock to "0 microlit o 10# DMSO in tu$es. From t!ere, I trans er 10 microlit rom t!e tu$e % 1"0microlit cell suspension in t!e &ell. '!is &ill gives me 100mcg(m) concentration. stock * 1 ml 100# DMSO +&(curcumin dissolved, 1st dilution 1-10 * 10# DMSO 2nd dilution +into cell culture, 1-20 * 10# .1(20 * 0./# DMSO +&(curcumin dissolved, or curcumin conc0n in g stock * 0.02g(1ml 1st dil0n 1-10 * 0.002g(ml 2nd diln 1-20 * 0.002g(ml .1(20* 0.0001g(ml or 0.1 mg(ml #DMSO * +10 .100#(100, % +"0.10(100, * 10# % "# * 1"#DMSO at 1st dilution. Makes sense $ecause "0ul at 10# &ould $e "ul o DMSO % 10ul 100# DMSO * 1"ul o DMSO ( 100ul total volume