Male Pheromone Disc Preparation

Males 14-20 days post-imaginal eclosion

Infected (A)
Implantation of 2mm nylon 4 Infected males (A) placed onto filter paper within a petri dish for 48h

Sterile Stab (B)

Insertion with sterile needle 4 Sterile stab males (B) placed onto filter paper within a petri dish for 48h

Control (C)
Similar handling procedure only. 4 Control males (C) placed onto filter paper within a petri dish for 48h

4x(A)

4x(B)

4x(C)

Males removed
(A)

Males removed
(B)

Males removed
(C)

Experimental Procedure
Females 16-22 days post-imaginal eclosion

Infected (A)

Control (C)

Treatment AA Female placed into petri dish previously prepared with infected male (A) pheromones for 24h
(A)

Treatment BA Treatment CA Treatment AC Female placed into Female placed into Female placed into petri dish previously petri dish previously petri dish previously prepared with sterile prepared with control prepared with stab male (B) male (C) infected male (A) pheromones for 24h pheromones for 24h pheromones for 24h
(B) (C) (A)

Treatment BC Treatment CC Female placed into Female placed into petri dish previously petri dish previously prepared with sterile prepared with control stab male (B) male (C) pheromones for 24h pheromones for 24h
(B) (C)

(A)

(A)

(A)

(C)

(C)

(C)

Female removed, Female removed, Female removed, Female removed, Female removed, Female removed, implanted with 2mm implanted with 2mm implanted with 2mm handled with similar handled with similar handled with similar nylon and returned to nylon and returned to nylon and returned to procedure and procedure and procedure and their petri dish for their petri dish for their petri dish for returned to their petri returned to their petri returned to their petri 24h 24h 24h dish for 24h dish for 24h dish for 24h

PO, proPO and encapsulation measured

PO, proPO and encapsulation measured

PO, proPO and encapsulation measured

PO and proPO measured

PO and proPO measured

PO and proPO measured