PROJECT REPORT ON "To study the quantity of casein present in different samples of milk"

Submitted to Mrs. PRABHJOT CHEMISTRY TEACHER Submitted By : Jaskaran Singh XII G

BUDHA DAL PUBLIC SCHOOL, PATIALA

ACKNOWLEGEMENT
AT THE VERY OUTSET I EXPRESS MY HEARTFUL GRATITUDE TO DR AMRIT AUJLA PRINCIPAL OF BUDHA DAL PUBLIC SCHOOL FOR GRANTING ME PERMISSION TO WORK ON MY PROJECT IN SCHOOL LABORATORY. I WOULD ALSO LIKE TO THANK MRS. PRABHJOT WHO HAS BEEN GUIDING FORCE FOR MY REPORT ON "TO STUDY THE QUANTITY OF CEASIN PRESENT IN DIFFERENT SAMPLE OF MILK". I WOULD ALSO LIKE TO THANKDS MY FRIENDS AND FAMILY FOR GIVING FULL SUPORT TO TWORK ON THIS PROJECT.

CERTIFICATE :
CERTIFIED THAT REPORT ENTITLED TO STUDY THE QUANTITY OF CAESIN PRESENT IN DIFFERENT SMAPLES OF MILK WHICH IS BEING SUBMITTED TIN THE PARTIAL FULLFILMENT OF REQUIRMENT FOR CERTIFICATE OF +2 NON MEDICAL B.D.P.S PATIALA, IS ARECORD OF THE CANDIDATES OWN WORK CARRIED OUT BY HER SUPERVISION AND GUIDENCE. THE MATTER EMBODED IN THE PROJECT HAS NOT BEEN SUBMITTED IN THE PART OR FULL OR ANY ANOTHER SCHOOL OR INSTITUTION.
Dr. AMRIT AUJLA PRINCIPAL BUDHA DAL PUBLIC SCHOOL Mrs. PRABHJOT CHEMISTRY TEACHER

INTRODUCTION
MILK IS A COMPLETE DIET AS IT CONTAINS IN ITS PROTEINS, CARBOHYDERATES, MINERALS, VITAMINS AND WATER. CAESIN IS THE MAJOR PROTEIN CONSTITUENT PRESENT IN MILK AND IS MIXED PHOSPHO PROTEIN. CAESIN HAS ISOELECTRIC PH ABOUT 47 AND CAN BE EASILY SEPRATED AROUND THIS PH. IT READILYDISOLVES IN DILUTE ACID AND ALKALIS CAESIAN PRESENT IN MILK AS CALCIUM CAESINA IN FORM OF MISCELLES. THERE MISCELLES HAVE NEGATIVE CHANGE ON ADDING ACID TO THE MILK. THE NEGATIVE CHARGES ARE NEUTRALISED.

PROCEDURE
TAKE A CLEANDRY BAEKER PUT INTO 20 ML OF COWS MILK TAKE ANOTHER BEAKER AND PUT 20ML OF BUFFALOWS MILK AND IN THE THIRD DEAKER T AKE 20ML OF SHEEPS MILK. ADD 20ML OF SALTURATE DMAMONIUM SULPHATE SOLUTION SLOWLY IN ALL THE THREE BAEAKERS WITH STIRRING FAT ALPONG WITH CAESIN WILL PRECIPITATE OUT. FILTER THE SOLUTION AND TREANSFER THE PRECIPITATES IN ANOTHER BEAKER. ADD 30ML OF WATER TO THE PRECIPITATES. ONLY CAESIN DISSOLVES IN WATER FORMING MILKY SOLUTION LEAVIANG FAT UNDISSOLVED. HEAT THE MILK SOLUTION TO ABOUT 40C ADD 1% AETIC ACID DROP WISE, WHEN CAESIN GET PRECIPITATED. FILTER THE PRECIPITATES WASH WITH WATER AND LET THE PRECIPITAES DRY. WEIGHT THE DRY SOLID MASS IN PR EVIOUSLY WEIGHED WASH GLASS.

TO STUDY THE QUANTITY OF CAESIN PRESENT IN DIFFERENT SAMPLES OF MILK

REQUIRMENTS :
BEAKER (250ML), FILTER PAPER, GLASS ROD, WEIGHT BOX, FILTERATION FALSK,BSCHNERFUNNEL, WATER PUMP, TEST TUBES, PROCELIAN DISH, BURNER, DIFFERENT SAMPLES OF MILK, ONE PERCENT ACETATE SOLUTION SATURATED AMMONIUM SULPHATE SOLUTION.

ADD 20 ML OF SATURATED AMMONIUM SULPHATE SOLUTION

OBSERVATIONS
VOLUME OF MILK TAKEN IN EACH CASE = 20ML
NAME OF MILK WEIGHT OF CAESIN 4 10 % OF CAESIN

COWS MILK BUFFALOWS MILK SHEEPS MILK

20% 50%

15%

CONCLUSION :
DIFFERENT SAMPLES OF MILK CONTAINS DIFFERENT % OF CAESIN.