Drawing of Enyzme Graphs

Y axis:
Dependent

Enzymes variable
(measured,
results)
Examples:
Revision
Rate of rxn,
amount of
product
formed X axis: Independent variable
(controlled by experimenter)/ Time
Examples: pH, substrate conc, temp

Drawing of Enzyme Graphs Interpreting graphs

• Use pencil and ruler to draw axes
• Use small crosses to mark points on graph
• Mark off intervals on axes to indicate scale,
must be suitable scale
• X and Y axis must be labelled (eg.Time,
Amount of product formed)
• Units must be added after label of axis (eg.
Time/s or Rate of reaction/g per s)
• Enzyme graphs have a trend: Join points with
a smooth curve (use curve rule if necessary)
• Check graph axes
• Common graphs include:
– Amt of product formed or amount of
substrate used against Time (Refer to
graph on Pg 12 of notes to revise)
– Rate of reaction against Temp, pH, Conc
of substrate

Rate of reaction against Temp Rate of reaction against pH

Inactivation: Only at
Denaturation:
very low temperatures. Due to change
Low rate of reaction in pH beyond
due to low KE,
optimum.
reversible process
Denaturation: Reversible with
Increase in % of small changes,
denatured irreversible with
enzyme with larger changes
increasing temp in pH
beyond optimum
temperature,
irreversible
process

1
 Rate of reaction against
substrate conc
Increasing enz conc:
Increase in collisions
between enz & substrate,
reaction rate increases
Saturation Point:
Enzymes saturated,
working at maximum rate
Other principles to note
• A reaction produces a fixed amount of
product, depending on the amount of
substrate used.
– Same amount of substrate used produces
same amount of product when reaction
reaches completion.
– Halving the substrate used produces half
the amount of product, and so on and so
forth.

Other principles to note

• Must quote data from graph in your
answer, eg:
– When does curve plateau, how much
product formed
• Must use dotted lines to show how you
obtain values from graphs, eg, when
reading off the graph to obtain
optimum pH of enzyme