LABORATORY REPORT No.

_____
MEDIA PREPARATION and QUALITY CONTROL

Name:__________________________________ Date Performed:________________

A. Preparation of media and inoculation of growth media.
Use the table below to design quality control of media for potability test of drinking water.
Test Culture Media Selectivity Performance
Test
Acceptance Criteria


Staphylococcus
aureus
BPA Selective Productivity >70% recovery of
target organism
BGLB Ecometric




Bacillus cereus
MYP







Salmonella
sp.
LB
TT
RV
BSA/HEA/XLD
TSI
LIA



Source:
Standard Methods for the Examination of Dairy Products
American Public Health Association
Chapter 4: Media and Dilution Water Preparation
B. SAMPLE MEDIA PREPARATION LOGSHEET
Record the weight and volume of each commercially prepared media used and complete the
form for MEDIA PREPARATION and QUALITY CONTROL. Use appropriate control strains for
growth performance testing of laboratory prepared
Test
Name of
Medium
Brand
Lot
Number
Amount
weighed
(g)
Volume
of
water
used
(ml)
pH
Sterility Control
Desired
incubat
ion
Temp
Room
Temp
+ -
Staphylococcus
aureus
BPW







Bacillus cereus






Salmonella













C. Answer the following questions in the space provided.

1. Why is it essential that media be sterile prior to use?



2. Why must agar be cooled prior to pouring plates?



3. Why are the inoculating loop and needle flamed before and after use?



4. What is contamination? What are the possible contaminants in the lab?



5. How does one determine if growth has occurred in broth?