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  • Mean Normal Prothombin Time (MNPT)

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    candiddreams
    476 vizualizări10 pagini
    This document discusses quality control procedures for routine coagulation tests. It defines mean normal prothrombin time (MNPT) as the geometric mean PT of a reference sample group, which is used as the denominator for calculating the international normalized ratio (INR). It recommends collecting citrated plasma from 20 healthy donors to establish the normal PT reference range. Quality control is done using commercially available lyophilized normal and abnormal controls or an in-house normal pooled plasma. The pooled plasma is prepared by mixing and storing aliquots of citrated plasma from at least six healthy donors. Control values and coefficients of variation are monitored to ensure test accuracy.

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    TMH proceedings 2010-2011,pdf

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    This document discusses quality control procedures for routine coagulation tests. It defines mean normal prothrombin time (MNPT) as the geometric mean PT of a reference sample group, which is used as the denominator for calculating the international normalized ratio (INR). It recommends collecting citrated plasma from 20 healthy donors to establish the normal PT reference range. Quality control is done using commercially available lyophilized normal and abnormal controls or an in-house normal pooled plasma. The pooled plasma is prepared by mixing and storing aliquots of citrated plasma from at least six healthy donors. Control values and coefficients of variation are monitored to ensure test accuracy.

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    © All Rights Reserved
    Descărcați ca PDF, TXT sau citiți online pe Scribd
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    476 vizualizări10 pagini

    Mean Normal Prothombin Time (MNPT)

    Încărcat de

    candiddreams
    This document discusses quality control procedures for routine coagulation tests. It defines mean normal prothrombin time (MNPT) as the geometric mean PT of a reference sample group, which is used as the denominator for calculating the international normalized ratio (INR). It recommends collecting citrated plasma from 20 healthy donors to establish the normal PT reference range. Quality control is done using commercially available lyophilized normal and abnormal controls or an in-house normal pooled plasma. The pooled plasma is prepared by mixing and storing aliquots of citrated plasma from at least six healthy donors. Control values and coefficients of variation are monitored to ensure test accuracy.

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    din 10

    Quality control for routine

    coagulation tests
    Mr.Shashikant G. Mahadik,
    Scientific Officer C,
    Heamatopathology, TMH
    What is Mean Normal Prothrombin Time
    (MNPT)?
    It is geometric mean of PT of reference sample
    group
    MNPT is the denominator used for calculation of
    International Normalized ratio (INR)
    MNPT is a must for any lab doing coagulation
    studies
    How do you do MNPT?
    Collect citrated plasma of apparently healthy
    individuals (20 donors). Do PT and APTT of all
    these 20 cases. Take a mean. Do plus/minus 2 SD
    to establish normal reference range of PT
    PT is reported in form of INR
    INR = (Test/MNPT)
    ISI
    For a small size laboratory: Commercially available
    lyophilized controls (normal and abnormal high)
    For large sized laboratory (or a hospital based
    laboratory): Commercially available lyophilized
    controls (normal and abnormal high) or in-house
    normal pooled plasma can be used
    Preparation of Normal Pooled Plasma
    Collect citrated plasma from minimum six
    healthy donors
    Pool them in a plastic container, mix and
    centrifuge at 1500 g for 10 min at RT
    Estimate platelet count (platelet poor plasma <10,000/cmm),
    check PT and APTT values (should be within normal range)
    Aliquot and store at -80 C
    Thaw immediately, analyze with every batch (as a QC
    sample)
    Monitor with successive runs
    New lot of pool plasma should be analyzed parallel with older
    lot
    Range, mean and +/- 2SD of pool plasma is calculated after
    20 successive readings
    If control values go outside acceptable
    ranges:
    - Check controls/pooled plasma for any deterioration
    - Check expiry and stability of reagents
    - Coagulometer problem
    Plot LJ chart and calculate %CV
    Based on monthly readings of pool plasma, plot LJ
    chart and calculate CVs
    Monitor results
    CV for our laboratory
    PT 7.0
    APTT 8.0
    THANK YOU

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