Phenols

Standard (mg/ml) Absorbance 1 Absorbance 2 Average

0.5000
1.0933
0.6008
0.8471
0.2500
0.8378
0.3517
0.5948
0.1250
0.1458
0.0938
0.1198
0.0625
0.0561
0.0933
0.0747
0.0313
0.0075
0.0397
0.0236
0.0156
0.0043
0.016
0.0102
0.0000
0
0.0013
0.0007

Sample
Black Bean 1.1
Black Bean 1.2
Black Bean 1.3
Black Bean 2.1
Black Bean 2.2
Black Bean 2.3
Black Bean 3.1
Black Bean 3.2
Black Bean 3.3
Black Bean 4.1
Black Bean 4.2
Black Bean 4.3
Black Bean 5.1
Black Bean 5.2
Black Bean 5.3
Black Bean 6.1
Black Bean 6.2
Black Bean 6.3
Black Bean 7.1
Black Bean 7.2
Black Bean 7.3
Black Bean 8.1
Black Bean 8.2
Black Bean 8.3
Black Bean 9.1
Black Bean 9.2
Black Bean 9.3
Black Bean 10.1
Black Bean 10.2
Black Bean 10.3
Black Bean 11.1
Black Bean 11.2
Black Bean 11.3
Black Bean 12.1
Black Bean 12.2

Solvent
75
75
75
50
50
50
75
75
75
50
50
50
50
50
50
50
50
50
50
50
50
75
75
75
25
25
25
75
75
75
50
50
50
25
25

Mass of Raw Volume of

Sample (g)
Extract (ml)
Time (min)
120
1.0000
120
1.0000
120
1.0000
120
1.0000
120
1.0000
120
1.0000
60
0.5000
60
0.5000
60
0.5000
60
1
60
1
60
1
120
1.5
120
1.5
120
1.5
180
1.0000
180
1.0000
180
1.0000
120
0.5000
120
0.5000
120
0.5000
180
1.5
180
1.5
180
1.5
180
0.5000
180
0.5000
180
0.5000
180
0.5000
180
0.5000
180
0.5000
120
1.0000
120
1.0000
120
1.0000
180
1.5
180
1.5

Page 1

Slope
Intercept
Correlation

1.83657945
-0.01959756
0.97374237

Dilution
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5

1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Absorbance
0.7146
0.7169
0.6436
0.4058
0.503
0.4279
0.7125
0.8224
0.7877
0.9389
0.9533
1.0228
0.9055
1.1168
0.8444
0.5502
0.5958
0.401
0.9146
1.2768
1.0989
0.3285
0.3031
0.3019
0.3629
0.4827
0.4487
0.6522
0.6404
0.6059
0.5192
0.5051
0.5411
0.8529
1.4566

Phenols
Black Bean 12.3
Black Bean 13.1
Black Bean 13.2
Black Bean 13.3
Black Bean 14.1
Black Bean 14.2
Black Bean 14.3
Black Bean 15.1
Black Bean 15.2
Black Bean 15.3
Black Bean 16.1
Black Bean 16.2
Black Bean 16.3
Black Bean 17.1
Black Bean 17.2
Black Bean 17.3
Black Bean 18.1
Black Bean 18.2
Black Bean 18.3

25
75
75
75
25
25
25
25
25
25
50
50
50
25
25
25
75
75
75

180
60
60
60
60
60
60
60
60
60
120
120
120
120
120
120
180
180
180

1.5
1.5
1.5
1.5
0.5000
0.5000
0.5000
1.5
1.5
1.5
1.0000
1.0000
1.0000
1.0000
1.0000
1.0000
1.5
1.5
1.5

Page 2

5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5

1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

1.0181
0.1193
0.1274
0.0876
0.1102
0.0701
0.6402
0.7441
0.6779
0.6721
0.5407
0.5316
0.4536
1.0533
1.1691
0.8596

Phenols

0.5000
0.2500
0.1250
0.0625
0.0313
0.0156
0.0000

0.8471
0.5948
0.1198
0.0747
0.0236
0.0102
0.0007

Concentratio
n in Test
Concentratio
Solution
n in Sample
(mg/ml)
(mg/g)
Average
0.3998
1.9988
0.401
2.005
0.361
1.806 1.936
0.232
1.158
0.285
1.423
0.244
1.218 1.266
0.399
3.986
0.458
4.585
0.440
4.396 4.322
0.522
2.609
0.530
2.649
0.568
2.838 2.699
0.504
1.679
0.619
2.063
0.470
1.568 1.770
0.310
1.551
0.335
1.675
0.229
1.145 1.457
0.509
5.087
0.706
7.059
0.609
6.090 6.078
0.190
0.632
0.176
0.586
0.175
0.584 0.600
0.208
2.083
0.273
2.735
0.255
2.550 2.456
0.366
3.658
0.359
3.594
0.341
3.406 3.552
0.293
1.467
0.286
1.428
0.305
1.526 1.474
0.475
1.584
0.804
2.679

Standard
Deviation

RSD

0.113

5.859

0.139

10.953

0.306

7.077

0.122

4.526

0.259

14.658

0.277

19.035

0.986

16.223

0.027

4.542

0.336

13.688

0.131

3.688

0.049

3.351

Page 3

Phenols
0.565
0.076
0.080
0.058
0.071
0.049
0.359
0.416
0.380
0.377
0.305
0.300
0.258
0.584
0.647
0.479
0.011
0.011
0.011

1.883
0.252
0.267
0.195
0.707
0.488
3.593
1.386
1.266
1.255
1.525
1.501
1.288
2.921
3.236
2.394
0.036
0.036
0.036

2.049

0.566

27.639

0.238

0.038

16.053

1.596

1.733

108.566

1.302

0.073

5.574

1.438

0.130

9.064

2.850

0.426

14.937

0.036

0.000

0.000

Page 4

Flavonoids

Standard (mg/ml) Absorbance 1 Absorbance 2 Average

0.2500
0.7224
0.737
0.7297
0.2500
0.3952
0.3859
0.3906
0.1250
0.1733
0.1898
0.1816
0.0625
0.0924
0.0901
0.0913
0.0313
0.0431
0.0409
0.0420
0.0156
0.0179
0.0206
0.0193
0.0000
-0.0006
0.0105
0.0050

Sample
Black Bean 1.1
Black Bean 1.2
Black Bean 1.3
Black Bean 2.1
Black Bean 2.2
Black Bean 2.3
Black Bean 3.1
Black Bean 3.2
Black Bean 3.3
Black Bean 4.1
Black Bean 4.2
Black Bean 4.3
Black Bean 5.1
Black Bean 5.2
Black Bean 5.3
Black Bean 6.1
Black Bean 6.2
Black Bean 6.3
Black Bean 7.1
Black Bean 7.2
Black Bean 7.3
Black Bean 8.1
Black Bean 8.2
Black Bean 8.3
Black Bean 9.1
Black Bean 9.2
Black Bean 9.3
Black Bean 10.1
Black Bean 10.2
Black Bean 10.3
Black Bean 11.1
Black Bean 11.2
Black Bean 11.3
Black Bean 12.1
Black Bean 12.2

Solvent
75
75
75
50
50
50
75
75
75
50
50
50
50
50
50
50
50
50
50
50
50
75
75
75
25
25
25
75
75
75
50
50
50
25
25

Mass of Raw Volume of

Sample (g)
Extract (ml)
Time (min)
120
1.0000
120
1.0000
120
1.0000
120
1.0000
120
1.0000
120
1.0000
60
0.5000
60
0.5000
60
0.5000
60
1
60
1
60
1
120
1.5
120
1.5
120
1.5
180
1.0000
180
1.0000
180
1.0000
120
0.5000
120
0.5000
120
0.5000
180
1.5
180
1.5
180
1.5
180
0.5000
180
0.5000
180
0.5000
180
0.5000
180
0.5000
180
0.5000
120
1.0000
120
1.0000
120
1.0000
180
1.5
180
1.5

Page 5

Slope
Intercept
Correlation

2.28476102
-0.03123162
0.91947766

Dilution
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5

1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Absorbance
1.7228
2.4565
2.0029
1.2357
0.9287
1.0903
0.9652
0.9055
0.9486
1.3695
1.1048
1.3107
1.5621
1.6373
1.41
1.2153
1.4867
1.2911
0.7741
0.6832
0.6234
2.086
1.9449
2.1752
0.3359
0.324
0.3276
0.5652
0.8631
0.8857
1.0251
1.277
1.1109
0.5434
0.5601

Flavonoids
Black Bean 12.3
Black Bean 13.1
Black Bean 13.2
Black Bean 13.3
Black Bean 14.1
Black Bean 14.2
Black Bean 14.3
Black Bean 15.1
Black Bean 15.2
Black Bean 15.3
Black Bean 16.1
Black Bean 16.2
Black Bean 16.3
Black Bean 17.1
Black Bean 17.2
Black Bean 17.3
Black Bean 18.1
Black Bean 18.2
Black Bean 18.3

25
75
75
75
25
25
25
25
25
25
50
50
50
25
25
25
75
75
75

180
60
60
60
60
60
60
60
60
60
120
120
120
120
120
120
180
180
180

1.5
1.5
1.5
1.5
0.5000
0.5000
0.5000
1.5
1.5
1.5
1.0000
1.0000
1.0000
1.0000
1.0000
1.0000
1.5
1.5
1.5

Page 6

5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5

1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

0.5395
2.2253
2.5523
2.0475
0.4027
0.3603
0.3024
0.3516
0.2339
0.7274
0.9655
1.1463
1.0565
0.5538
0.5035
0.47
0.5651
2.0014
1.7671

Flavonoids

Concentratio
n in Test
Concentratio
Solution
n in Sample
(mg/ml)
(mg/g)
Average
0.768
3.839
1.089
5.444
0.890
4.452 4.578
0.555
2.773
0.420
2.101
0.491
2.454 2.443
0.436
4.361
0.410
4.100
0.429
4.289 4.250
0.613
3.065
0.497
2.486
0.587
2.937 2.829
0.697
2.325
0.730
2.434
0.631
2.103 2.287
0.546
2.728
0.664
3.322
0.579
2.894 2.981
0.352
3.525
0.313
3.127
0.287
2.865 3.172
0.927
3.089
0.865
2.883
0.966
3.219 3.064
0.161
1.607
0.155
1.555
0.157
1.571 1.577
0.261
2.610
0.391
3.914
0.401
4.013 3.513
0.462
2.312
0.573
2.863
0.500
2.499 2.558
0.252
0.838
0.259
0.863

Standard
Deviation

RSD

0.810

17.699

0.336

13.759

0.135

3.174

0.304

10.751

0.169

7.386

0.306

10.280

0.332

10.470

0.169

5.530

0.027

1.693

0.783

22.288

0.280

10.956

Page 7

Flavonoids
0.250
0.988
1.131
0.910
0.190
0.171
0.146
0.168
0.116
0.332
0.436
0.515
0.476
0.256
0.234
0.219
0.261
0.890
0.787

0.833
3.292
3.769
3.033
1.899
1.714
1.460
0.559
0.387
1.107
2.181
2.577
2.380
1.280
1.170
1.097
0.870
2.965
2.624

0.845

0.016

1.890

3.365

0.374

11.102

1.691

0.220

13.032

0.684

0.376

54.974

2.380

0.198

8.314

1.182

0.092

7.806

2.153

1.124

52.215

Page 8

ORAC
Trolox Concentration (mg/ml)
0.1
Trolox as m/ml
in Standard
0.399536538 42.5771116
0.199768269 27.7300183
0.099884134
17.017994 Slope
0.049942067 10.3323443 Intercept
0.024971034 6.40976488 Correlation
0.012485517 3.74135563
0.006242758 2.55776565
0.003121379 0.98163232
Units are micromolar Trolox equivalents per gram

104.111859
3.55999701
0.98570358

Samples

Sample
Black Bean 1.1
Black Bean 1.2
Black Bean 1.3
Black Bean 2.1
Black Bean 2.2
Black Bean 2.3
Black Bean 3.1
Black Bean 3.2
Black Bean 3.3
Black Bean 4.1
Black Bean 4.2
Black Bean 4.3
Black Bean 5.1
Black Bean 5.2
Black Bean 5.3
Black Bean 6.1
Black Bean 6.2
Black Bean 6.3
Black Bean 7.1
Black Bean 7.2
Black Bean 7.3
Black Bean 8.1
Black Bean 8.2
Black Bean 8.3
Black Bean 9.1
Black Bean 9.2
Black Bean 9.3
Black Bean 10.1
Black Bean 10.2
Black Bean 10.3
Black Bean 11.1
Black Bean 11.2
Black Bean 11.3
Black Bean 12.1

Solvent
75
75
75
50
50
50
75
75
75
50
50
50
50
50
50
50
50
50
50
50
50
75
75
75
25
25
25
75
75
75
50
50
50
25

Mass of Raw Volume of

Sample (g)
Extract (ml)
Time (min)
120
1.0000
120
1.0000
120
1.0000
120
1.0000
120
1.0000
120
1.0000
60
0.5000
60
0.5000
60
0.5000
60
1
60
1
60
1
120
1.5
120
1.5
120
1.5
180
1.0000
180
1.0000
180
1.0000
120
0.5000
120
0.5000
120
0.5000
180
1.5
180
1.5
180
1.5
180
0.5000
180
0.5000
180
0.5000
180
0.5000
180
0.5000
180
0.5000
120
1.0000
120
1.0000
120
1.0000
180
1.5

Page 9

Dillution
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5

50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50

Area Under
Curve
34.414
38.775
36.110
31.224
45.653
39.127
25.321
24.636
28.460
48.367
45.828
50.216
54.385
59.098
52.438
50.578
54.986
49.773
26.743
30.949
28.289
40.638
43.805
47.271
20.869
20.047
21.318
20.452
25.592
24.525
41.117
45.628
47.967
34.052

ORAC
Black Bean 12.2
Black Bean 12.3
Black Bean 13.1
Black Bean 13.2
Black Bean 13.3
Black Bean 14.1
Black Bean 14.2
Black Bean 14.3
Black Bean 15.1
Black Bean 15.2
Black Bean 15.3
Black Bean 16.1
Black Bean 16.2
Black Bean 16.3
Black Bean 17.1
Black Bean 17.2
Black Bean 17.3
Black Bean 18.1
Black Bean 18.2
Black Bean 18.3

25
25
75
75
75
25
25
25
25
25
25
50
50
50
25
25
25
75
75
75

180
180
60
60
60
60
60
60
60
60
60
120
120
120
120
120
120
180
180
180

1.5
1.5
1.5
1.5
1.5
0.5000
0.5000
0.5000
1.5
1.5
1.5
1.0000
1.0000
1.0000
1.0000
1.0000
1.0000
1.5
1.5
1.5

Page 10

5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5

50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50
50

34.531
39.201
43.529
47.462
39.981
22.960
23.574
22.733
22.922
15.434
52.945
41.291
45.765
45.610
36.290
35.725
29.963
45.978
43.907
40.383

ORAC

uM Trolox/ml uM Trolox/g
Standard
Test Extract Sample
Average
Deviation
RSD
14.818
74.089
16.912
84.560
15.632
78.160
78.936
5.279
6.687
13.286
66.429
20.215
101.076
17.081
85.406
84.304
17.350
20.580
10.451
104.507
10.122
101.220
11.958
119.581
108.436
9.791
9.029
21.519
107.594
20.299
101.497
22.407
112.034
107.042
5.290
4.942
24.409
81.363
26.672
88.908
23.474
78.245
82.839
5.482
6.618
22.581
112.903
24.697
123.486
22.194
110.970
115.786
6.738
5.819
11.134
111.336
13.153
131.535
11.876
118.762
120.544
10.217
8.475
17.807
59.356
19.328
64.426
20.992
69.974
64.586
5.311
8.223
8.313
83.129
7.918
79.177
8.528
85.283
82.530
3.097
3.752
8.112
81.124
10.581
105.811
10.069
100.687
95.874
13.028
13.589
18.037
90.184
20.203
101.017
21.327
106.634
99.278
8.361
8.422
14.644
48.813

Page 11

ORAC
14.874
17.117
19.195
21.084
17.491
9.317
9.612
9.208
9.299
5.702
23.717
18.121
20.269
20.195
15.719
15.447
12.680
20.371
19.377
17.684

49.580
57.055
63.985
70.280
58.304
93.170
96.118
92.080
30.995
19.008
79.058
90.603
101.345
100.974
78.594
77.237
63.401
67.904
64.589
58.948

51.816

4.554

8.788

64.190

5.991

9.333

93.790

2.089

2.227

43.020

31.780

73.871

97.641

6.098

6.245

73.077

8.407

11.505

63.814

4.528

7.096

Page 12