DEFINITION OF TERMS

Extract
To draw or pull out, using force or effort.
To obtain from a substance by chemical or mechanical action, as by pressure, distillation, or eva
poration.
a concentrated form of an herb that is derived when the crude herb is mixed with water, alcohol, or anoth
er solvent and distilled orevaporated. Extracts may be either fluid or solid.
n in herbal medicine, a substance derived by mixing an herb with a solvent (usually a mixture of water an
d alcohol).

ALTERNATIVE
A choice limited to one of two or more possibilities
One of possibilities that can be chosen
Affording the choice

COUNTERSTAIN
A second stain added to a previously stained tissue sample to make cellular details more distinct
A stain applied to render the effects of another stain more discernible
A stain of a contrasting color used to color the components in a microscopic specimen that are not made
visible by the principal stain

HYPOTHESIS(null)
Alugbati fruit extract is not a good alternative stain to wright-giemsa for blood smears
HYPOTHESIS
Alugbati gives good staining quality for routine hematological blood smears

STATEMENT OF THE PROBLEM
Can alugbati stain be used as haematological stain?
Does it stain all blood cells: erythrocytes,leukocytes,thrombocytes
What substance can enhance the staining properties of alugbati?
Does it provide differential staining of white blood cells(leukocytes)
Can the stain be used for pathological blood samples like in malarial smears?

OBJECTIVES
To improvise an alternative stain to wright-giemsa
To provide an alternative stain made from an alugbati that is common in the Philippines
To produce a less expensive stain for blood smears
To minimize the toxicity of the stain

SIGNIFICANCE OF THE STUDY
Future researchers, who will make a further study about bilirubin that might help
and may encourage them more to develop innovations the method used in the
study.