such as proteins, carbohydrates, lipids, and nucleic acids, Biomolecule stretch of DNA and/or RNA that codes for a polypeptide or for an RNA chain that has a function in the organism. Gene nucleotides Various substances are required for the proper functioning of the coagulation (clotting) cascade. biological molecules that form the building blocks of nucleic acids DNA and RNA! and serve to carry packets of energy "ithin the cell A#$! blood clotting factors genetic engineering direct manipulation of an organism%s genome using biotechnology small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA "ithin a cell plasmid cell basic structural and functional unit of all kno"n living organisms. peptide hormone that stimulates gro"th, cell reproduction and regeneration in humans and other animals. human gro"th hormone se&uence large biological molecules consisting of one or more chains of amino acids. $roteins perform a vast array of functions "ithin living organisms, including cataly'ing metabolic reactions, replicating DNA, responding to stimuli, informational molecule encoding the genetic instructions used in the development and functioning of all kno"n living organisms and many viruses. DNA deo(yribonucleic acid! succession of letters that indicate the order of nucleotides within a DNA (using GACT) or RNA (GAC) !olecule $roteins method for separation and analysis of macromolecules DNA, RNA and proteins! and their fragments, based on their si'e and charge gel electrophoresis instrument used to accuratel" !easure and dispense s!all #olu!es of liquid. micropipette the step"ise dilution of a substance in solution. )sually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion serial dilutions *himera single organism usually an animal! that is composed of t"o or more di+erent populations of genetically distinct cells that originated from di+erent 'ygotes involved in se(ual reproduction. ubi&uitous family of large biological molecules that performs multiple vital roles in the coding, decoding, regulation, and e(pression of genes Ribonucleic acid RNA! Diabetes mellitus type , formerly insulin dependent or -uvenile diabetes! is a form of diabetes mellitus that results from autoimmune destruction of insulin.producing beta cells of the pancreas peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. /nsulin Glucose simple monosaccharide found in plants. /t is one of the three dietary monosaccharides, along "ith fructose and galactose, that are absorbed directly into the bloodstream during digestion molecule most often found on the surface of a cell, "hich receives chemical signals originating e(ternally from the cell. receptor Diabetes mellitus type 0 formerly noninsulin.dependent diabetes mellitus N/DD1! or adult.onset diabetes! is a metabolic disorder that is characteri'ed by high blood glucose in the conte(t of insulin resistance and relative insulin de2ciency. gelatinous substance derived by boiling a polysaccharide in red algae, that provides a solid surface containing medium for the gro"th of bacteria and Agarose a portion of a total amount of a solution Ali&uot