Experiment-1:

Phytochemistry-II

Phytochemical Analysis Of Chamomile Flower

Information of medicinal plant:
Scientific name: 'Matricaria chamomilla'
Common name: Chamomile flower
Family name: Asteraceae

Extraction:
Methanolic Extraction

Aqueous Extraction

-2 gm plant + 20 ml alcohol in beaker

-2 gm plant in beaker + 20 ml D.W in beaker

-Put the beaker in water bath with covering by

-Put the beaker in water bath with covering by

aluminum foil for 5-10 min with add 3ml Hcl.

aluminum foil for 5-10 min.

- Cool the beaker and filter.

- Cool the beaker and filter.

Identification some Phytochemicals in chamomile flower:

Phytochemicals
General identification

Glycosides
Anthraquinone .glycosides

Polyphenols

Simple
(hydrolysable)
Tannins Condensed
(Unhydrolysable)

Flavonoids
Alkaloid

Reagents
-2 ml of glacial acetic
acid (CH3COOH) +
-1 ml dil. sulphuric
acid (H2SO4)
-1ml NH4OH + 1ml
KOH
- 3 drops of FeCl3 sol
(5%)
- 1 ml of dil. ammonia
(NH4OH)
-Few drops dil. HCl
0.1 M +
- Few drops of

dragendroff reagent

Pharmacological uses:
- Antibacterial, antiseptic, antthelminthic, antiviral.
- Anti-spasmodic
- Anti-inflammatory
- Antioxidant

Plant
extract
2 ml

1 ml

Results
Brown ring
coloration
Brownish red

5 ml

Blue black
coloration
Brownish green
coloration

5 ml

Yellow coloration

5 ml

Orange precipitate