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Experiment 2:
Qualitative Analysis
Pharmacological action:-(1)
- Anti-inflammatory(1)
- Antioxidant(1)
- Antibacterial activity(2)
- Beneficial to most pathological conditions which includes, high cholesterol and
antiinflammation; complications related to diabetes and cancer prevention.(1)
Due to containing Anthocyanins and hydroxycinnamic acids
Extraction:
Methanolic Extraction
Aqueous Extraction
Phytochemistry-II (Practical)
Glycosides:
Cardiac glycoside
(Keller-Killani test)
Anthraquinone .glycosides
Reagents
-2 ml of glacial acetic acid
(CH3COOH) +
-1 ml dil. sulphuric acid (H2SO4)
- 2 ml of glacial acetic acid
- 1 drop of feCl3 sol.
- 1 ml conc. H2SO4
Plant
extract
Results
2 ml
5 ml
1ml
Simple
(hydrolysable)
Tannins
Polyphenols:
Brownish red
Condensed
(Unhydrolysable)
Flavonoids
Notes
5ml
Yellow coloration
Alkaloid
5ml
Orange precipitate
Amino Acids:
1ml
- 2 ml of chloroform
-3ml conc. H2SO4
5 ml
References :
(1) Rekah, S.S. & Bhaskar, M. (2013). In Vitro Screening And Identification Of Antioxidant Activities of Orange (Citrus Sinensis) Peel
Extract In Different Solvents. International Journal of Pharma and Bio Sciences, 4(4):405-412.
(2) Arora, M. & Kaur, P. (2013). Phytochemical Screening Of Orange Peel And Pulp. International Journal of Research in Engineering and
Technology, 2(12):517-522.
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