Phytochemistry-II (Practical)

Pharm. Sultan Alkaleh

Experiment 2:

Qualitative Analysis

Phytochemical analysis of Hops

Information of medicinal plant :
Scientific name: Humulus lupulus
Common name: Hops
Family name: Canabinaceae

Phytochemicals orange peels:(1)

- Alkaloids
- Steroids
- Tanins
- Flavonoids
- Phenols
- Polyphenols.

Pharmacological action:-(1)
- Anti-inflammatory(1)
- Antioxidant(1)
- Antibacterial activity(2)
- Beneficial to most pathological conditions which includes, high cholesterol and
antiinflammation; complications related to diabetes and cancer prevention.(1)
Due to containing Anthocyanins and hydroxycinnamic acids

Extraction:
Methanolic Extraction

Aqueous Extraction

-2gm plant + 20ml alcohol in beaker

-2gm plant in beaker + 20ml D.W in beaker

-Put the beaker in water bath with covering by

-Put the beaker in water bath with covering by

aluminum foil for 5-10 min with add 3ml Hcl.

aluminum foil for 5-10 min.

- Cool the beaker and filter.

- Cool the beaker and filter.

Phytochemistry-II (Practical)

Pharm. Sultan Alkaleh

20 ml alcohol
2 gm plant

Cool

Heat for 20 min.

Filter

Beaker

Phytochemistry-II (Practical)

Pharm. Sultan Alkaleh

Identification some Phytochemicals in chamomile flower

Phytochemicals
General identification

Glycosides:

Cardiac glycoside
(Keller-Killani test)

Anthraquinone .glycosides

Reagents
-2 ml of glacial acetic acid
(CH3COOH) +
-1 ml dil. sulphuric acid (H2SO4)
- 2 ml of glacial acetic acid
- 1 drop of feCl3 sol.
- 1 ml conc. H2SO4

Plant
extract

Results

2 ml

Brown ring coloration

5 ml

Brown ring (2)

1ml

-1ml NH4OH + 1ml KOH

Simple
(hydrolysable)

Tannins
Polyphenols:

Brownish red

Blue black coloration

- 3 drops of FeCl3 sol (5%)

5ml

Condensed
(Unhydrolysable)

Flavonoids

Notes

Brownish green coloration

- 1 ml of dil. ammonia (NH4OH)

5ml

Yellow coloration

Alkaloid

-Few drops dil. HCl 0.1 M +

- Few drops of dragendroff reagent

5ml

Orange precipitate

Amino Acids:

Few drops of Ninhydrin reagent.

1ml

Purple Color (2)

Terpenoids (Salkowski test)

- 2 ml of chloroform
-3ml conc. H2SO4

5 ml

Reddish brown coloration (2)

References :
(1) Rekah, S.S. & Bhaskar, M. (2013). In Vitro Screening And Identification Of Antioxidant Activities of Orange (Citrus Sinensis) Peel
Extract In Different Solvents. International Journal of Pharma and Bio Sciences, 4(4):405-412.
(2) Arora, M. & Kaur, P. (2013). Phytochemical Screening Of Orange Peel And Pulp. International Journal of Research in Engineering and
Technology, 2(12):517-522.
3