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Guanosine has a maximum absorbance of 275 nm.

275 = 8400 M-1 cm-1 and the


path length is 1 cm. Using a spectrophotometer, you find the that 275= 0.70.
What is the concentration of guanosine?
Using Beer Lambert's Law,

.70 = (8400 M-1 cm-1)(1 cm)( )


Dividing by [(8400 M-1 cm-1)(1 cm)]
= 8.33x10-5mols/L

A solution in a cell of length 1 cm has an absorbance of the solution at a


particular wavelength using a spectrometer. The value is 1.92. Find a value
for molar absorptivity in a table of 19400 for that wavelength.
Substituting those values:

If a standard solution of 0.100 M Co2+ has an absorbance reading


of 0.464, what concentration (M) of Co2+ would have an
absorbance reading of 0.565?
A = abc
0.464 = a (1) 0.100 solve for a- that's the absorptivity constant;
then, plug in the 0.565
0.565= a (1) c; the a you calc'd from the first part. Assume b=1 in
both cases, because you'll probably use the same cuvette
thickness.

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