path length is 1 cm. Using a spectrophotometer, you find the that 275= 0.70. What is the concentration of guanosine? Using Beer Lambert's Law,
.70 = (8400 M-1 cm-1)(1 cm)( )
Dividing by [(8400 M-1 cm-1)(1 cm)] = 8.33x10-5mols/L
A solution in a cell of length 1 cm has an absorbance of the solution at a
particular wavelength using a spectrometer. The value is 1.92. Find a value for molar absorptivity in a table of 19400 for that wavelength. Substituting those values:
If a standard solution of 0.100 M Co2+ has an absorbance reading
of 0.464, what concentration (M) of Co2+ would have an absorbance reading of 0.565? A = abc 0.464 = a (1) 0.100 solve for a- that's the absorptivity constant; then, plug in the 0.565 0.565= a (1) c; the a you calc'd from the first part. Assume b=1 in both cases, because you'll probably use the same cuvette thickness.