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Sordaria Meiosis Lab

Sample of Sordaria in petri dish

Crossing-over in Sordaria sp.

When mycelia of these
two different strains
come together and
undergo meiosis, the
asci that develop will
contain four black
ascospores and four tan

Sordaria fimicola is an ascomycete fungus

Sordaria is a haploid (n) organism for most of its life
When the mycelium from two individuals meet, a
diploid zygote (2n) is formed.
The diploid zygote then undergoes meiosis to yield 8
haploid ascospores. These ascospores exist in a
narrow pod called an ascus (plural, asci). Many asci
will grow together forming a reproductive structure
called a perithecium.
When ascospores are mature the ascus ruptures,
releasing the ascospores. Each ascospore can
develop into a new haploid fungus.

Sample of all black spores

To the right is a photo
of asci that resulted
from a cross between
two black strains that
is both parental
strains had black
colored spores
All of the 8 asci
spores are black

Sample of tan spores

To the right is a photo
of asci that resulted
from a cross between
two tan parental
All of the 8 asci
spores are tan.

Black and tan cross

To the right is a photo
of asci that resulted
from a cross between
black and tan strains.
Some asci have 4
black spores and then
4 tan (4:4)
Some asci display a
2:4:2 or 2:2:2:2

No Recombination Asci 4:4

Recombinant Asci
(Crossing over has occurred)
2:2:2:2 or 2:4:2

Sample Problem

the number
that have
the number
of asciofthat
are looking
for 4:4orcombination
You are
for 2:2:2:2
2:4:2 combination.

7 asci display
crossing over

4 asci display
crossing over
11 total asci

Part II: Crossing Over in Sordaria

Now its your turn!

Directions for lab

Observe 10 photos of Sordaria asci. (include the
sample problem slide as #10)
Count 100 asci. Keep track of the number of
crossover, and no crossover asci that you see.
Do not count the asci that are entirely one color
(ie, all tan spores or all black spores), there
represent a double crossover!
Fill in the data table and answer lab questions.

Data Table
Slide #
& Sample
as slide 10

# asci 4:4

# asci

Total # asci % showing

Divided by

Gene to
(map units)

Copy data table in lab notebook make it large enough to
fit 10 slides.

Calculating average crossing over and

distance between the gene and centromere
(number of crossover) = X 100 = % crossover (total
% crossover = distance between gene & centromere

Lab Questions
What are homologous chromosomes?
What does crossing over ensure?
How many cells and what type of cells are created from
What does the term diploid mean? Haploid?
Why is meiosis so important to sexual reproduction?
Discuss how a 4:4 spore arrangement is made.
Using a diagram, show how you would get a 2:4:2 spore
Also answer questions1-5 form lab notebook on p.96
Complete Lab and turn in copies tomorrow