Sunny Park

Biology 11, Honors

Professor Rodriguez
March 8, 2015
Food and Digestion Lab Answer Sheet
Key Organs and Enzymes of the Digestive System
1. The salivary glands produce salivary amylase to digest carbohydrates.
2. The stomach produces pepsin, which is a protease to digest proteins.
3. The pancreas produces:
Proteases, to digest proteins within the small intestine
Pancreatic amylase to digest carbohydrates within the small intestine
Pancreatic lipase to digest lipids the small intestine

Part 1: Testing for Macromolecules

Hypotheses
Food 1 (Junk): Chocolate

Sugar
Fat/Lipids

Food 2 (Junk): Doritos

Fat/Lipids
Sugar
Starch

Food 3 (Junk): Rice Krispies

Sugar
Starch
Fat/Lipids

Food 4 (Healthy): Strawberry

Sugar

Food 5 (Healthy): Apple

Sugar

Food 6 (Healthy): Peanuts

Protein
Lipids

Directions for Testing

1. Why is it important to use a negative and positive control?
It is important to use a negative control to prevent false positive results make sure
the test works, and to prevent false negative results.
2. Use the following table to record your results for all 4 tests. Record both the color of the
resulting solution and whether the test is + or - for the molecule being tested.
Food

Benedicts

Iodine

Biuret

Sudan IV

(simple
sugar)

(starch)

(proteins)

(lipids)

Color

+
or
-

Color

+
or
-

Color

+
or
-

Color

+
or
-

Positive
Control

--

Red

Black

Purple

red

Negative
Control

--

Blue

Orange

clear blue

clear

Food 1

Doritos

Greenish
Brown

Dark Blue

light orange

Red
orange

Food 2

Rice
Krispies

Yellow
Orange

Dark
PurplishBlack

white

light
pink

Food 3

Dark
Chocolate

BlueGreen

Dark
Brown

Dark brown

brown

Food 4

Strawberry

Dark
Orange

Red
Orange

yellow

red

Food 5

Peanuts

Blue

Green

white (purple
on the bottom
layer of the

light
pink

tube)
Food 6

Apple

Red
Orange

Yellow
Orange

yellow-green

clear
pink

What conclusions can you draw about the nutrients found in the foods you tested?
1. All the healthy foods we tested (strawberry, peanuts, apple) contain lipids
2. All the junk foods we tested (dark chocolate, doritos, and rice krispies) contain
starch
3. All the junk foods we tested (dark chocolate, Doritos, and Rice Krispies) were tested
negative for protein
4. Among the healthy foods we tested, the fruits(strawberry and apple) contains simple
sugar whereas the peanuts do not.

Part 2: Digestion of Food

1. Fill in the following tables based on your results in lab
S1

S2

S3

Natural Color of test

tube

Clear

Clear

Clea
r

Iodines Reaction
color

orange

blackish

clear

Is there presence of
starch? Y/N

no

yes

no

G1

Unknown-S

G2

G3

G4

Clear

Clear

Clea
r

Clear

Benedict's Reaction
color

Clear Blue

Clear
Blue

Clea
r

Orange

Is there presence of

no

no

no

yes

Natural Color of test

tube

UnknownG

glucose? Y/N
2. Which tubes successfully digested starch?
Tubes S1 and S3 digested starch successfully. Tube G3 had no presence of starch so it was
not able to fully convert to glucose.

Part 3: Post-Lab thought experiment

1. Billy was super hungry after class, so he stopped at In-n-Out and ordered a #3 (Doubledouble, fries, and a strawberry shake). As he is eating he wonders how his tasty meal is
being digested. Using the word bank below, create a diagram showing Billy how his meal
moves through his digestive tract (from mouth to anus) and how his body works to digest
it.

2.

Challenge question, if Billy ate a bunch of Tums (antacids) BEFORE eating at In-n-Out
would his digestion be affected? If so, how? Be sure to describe how the enzymes
would/could be affected and why.
Yes, Billys digestion would be affected. In our modern diet, many of us are lacking in
enzymes. Foods such as In-N-Out lack natural enzymes that help us properly digest food.
In a normal digestion, the stomach with a normal pH of 3 would intake proteins and
necessary components of the food to break down amino acids so that our body will be able
to absorb essential vitamins and minerals for us to perform our daily tasks. Consuming
antacids would over-trigger acid secretion which can cause constipation.