Documente Academic
Documente Profesional
Documente Cultură
ARTICLE
By
Ni Kadek Wahyuni Antari
1213031002
A
Nama mahasiswa
NIM
: 1213031002
Tanggal
: 23 Maret 2015
: Kromatografi Kertas
No.
Bobot
Skor
(%)
(0-100)
Format
Abstract
10
3.
Introduction
20
4.
10
5.
30
6.
Conclusion
10
7.
Acknowledgment
8.
References
9.
Clear
Total score
100
Bobo x skor
INTRODUCTION
Amino acids are organic molecules
with low molecular mass (100-200 Da)
containing at least one carboxyl group (COOH) and an amino group (-NH2). Amino
acids have an important role for human life,
ranging from ensuring that cell division occurs
perfectly, metabolic functions, maintaining
memory, growth, healing up as the building
blocks of proteins are linear chains of amino
acids (Tika, 2010). In general, the amino acid is
obtained as a result of hydrolysis of proteins,
using either enzymes or acid. In this way is
obtained a mixture of a variety of amino acids
and to determine the type and quantity of each
amino acid should be a separation between the
amino acids. There are several methods to
analysis the type quantity of each amino, e.g.
gravimetric, microbiology, chromatography,
and electrophoresis methods. Furthermore,
each type of amino acids has different
properties. Beside differ in isoelectrical point,
they also differ in distribution coefficient (Rf).
To separate the constituent components of the
sample, chromatography paper can be done.
Chromatography comes from the word
"chroma" and "graphein". In Greek, the word
means both "color" and "write (Tika, 2010).
The process of chromatography was discovered
in 1903 by Mikhail Tswett, who separated
O
R
OH
+
OH
H2N
C
COOH
(aq)
(aq)
OH
(aq)
Ninhydrin Reducted
O
Ninhydrin
H
H
OH
+
OH
(aq)
N
H
H (aq)
O
C
N
HO
C
O
(aq)
(aq)
variables,
including
solvent
system,
temperature, length of run, and type of paper.
Because it is influenced by many variables, the
Rf of a compound that has been known to be
used as a standard or benchmark shopped
determine Rf other compounds. For qualitative
analysis, certain compounds are already known
to be used in conjunction with the compound to
be identified. Two different compounds in a
given solvent system can have the same Rf
value. Therefore, the analysis results obtained
by paper chromatography techniques must be
justified by other methods.
Table 1. The result of paper chromatography by uisng eluent of mixture of n-butanol, distilled water,
and glacial acetic acid
Distance taken by
Distance taken by
Sample
component of
Rf
eluent (cm)
mixture (cm)
Phenylalanine
5.2
8.6
0.60
Glycine
1.6
8.6
0.19
Tryptophan
4.9
8.6
0.57
L-cysteine
1.7
8.6
0.20
1.5
8.6
0.17
Unknown sample A
2.0
8.6
0.23
1.5
8.6
0.17
Unknown sample C
4.8
8.6
0.56
5.0
8.6
0.58
Discussion
In this experiment, carried out paper
chromatography to determine the amino acids
OH
+
OH
H2N
C
COOH
(aq)
(aq)
(aq)
Ninhydrin Reducted
OH
O
Ninhydrin
H
H
OH
+
OH
(aq)
N
H
H (aq)
O
C
N
HO
C
O
(aq)
(aq)
Figure 6. Ninhydrin reacts with amino acids produce purple colored compound
REFERENCES
Poedjadi, Anna dan Titin Supriyanti. 1994.
Dasar-Dasar
Biokimia.
Jakarta:
Universitas Indonesia
Redhana, I Wayan., Siti, Maryam. 2003.
Penuntun Praktikum Biokimia. Singaraja :
IKIP NEGERI SINGARAJA
Soebagio, Endang Budiasih, Sodiq Ibnu,
Hayuni Retno Widarti, Munzil. 2003.
Common Textbook (Edisi Revisi)
Kimia Analitik II. Malang: Universitas
Negeri Malang
Tika, I Nyoman. 2010. Buku Penuntun
Praktikum
Biokimia.
Singaraja:
Universitas Pendidikan Ganesha
Voet, D., Voet, J. G., & Pratt, C. W. (2013).
Fundamentals of biochemistry; life
at the molecular level. Danvers:
John Wiley & Sons, Inc.