THE EFFECTS OF PESTICIDES ON E.

COLI

The effects of pesticides, such as Bayer Advanced Garden Fruit, Citrus and Vegetable
Insect Control, Bayer Natria Insect, Disease and Mite Control, and Ready-to-Use
Multipurpose Garden Insect Killer on bacteria found on fruit and vegetables such as E.
coli.
Hunter Galindo and Silva Topchyan
West Career and Technical Academy

Author Note
Hunter Galindo, Biomedical Sciences, West Career and Technical Academy; Silva
Topchyan, Biomedical Sciences, West Career and Technical Academy.
Correspondence concerning this article should be addressed to Silva Topchyan,
Biomedical Sciences, West Career and Technical Academy, Las Vegas, Nevada, 89135.
Contact: silvat182@westcta.ccsd.net

THE EFFECTS OF PESTICIDES ON E. COLI

THE EFFECTS OF PESTICIDES ON E. COLI

THE EFFECTS OF PESTICIDES ON E. COLI

We recommend this thesis

prepared under our supervision by
Hunter Galindo and Silva Topchyan
entitled
The effects of pesticides, such as Bayer Advanced Garden Fruit, Citrus and
Vegetable Insect Control, Bayer Natria Insect, Disease and Mite Control, and
Ready-to-Use Multipurpose Garden Insect Killer on bacteria found on fruit and
vegetables such as E.coli.
be accepted in partial fulfillment of the requirements of
Senior Research Project
Biomedical Sciences

Mr. Gonzales, Program Teacher

Mr. Rash, Program Teacher

THE EFFECTS OF PESTICIDES ON E. COLI

May 2015

THE EFFECTS OF PESTICIDES ON E. COLI

Acknowledgments
Thank you to West Career and Technical Academys Biomedical Sciences program for
giving us the opportunity to and funding to carry out this project.

THE EFFECTS OF PESTICIDES ON E. COLI

TABLE OF CONTENTS
TITLE PAGE...............................1
PROGRAM COVER PAGE.i
ACKNOWLEDGMENTS...ii
TABLE OF CONTENTS.......iii
LIST OF TABLES AND FIGURES......iv
LIST OF ABBREVIATIONS..v
ABSTRACT.2
THESIS3
REFRENCES.16
FIGURES, TABLES, AND APPENDICES..17
VITA..19
Hunter Galindos Resume.....19
Silva Topchyans Resume.21

THE EFFECTS OF PESTICIDES ON E. COLI

LIST OF TABLES AND FIGURES

FIGURE 1

MEASURMENTS OF THE SIZE OF THE LARGEST SINGLE E. COLI

COLONIES

IN

EACH

PETRI

DISH.17
FIGURE 2

CORRELATION BETWEEN PESTICIDE TRIALS AND SIZE OF

E. COLI COLONIES.17

FIGURE 3

CHANGE IN E .COLI S CONSISTENCY AND TEXTURE IN TRIAL A

TO A318

THE EFFECTS OF PESTICIDES ON E. COLI

10

LIST OF ABBREVIATIONS
E.coli

Escherichia coli

THE EFFECTS OF PESTICIDES ON E. COLI

11

Abstract
Pesticides/chemicals are a major use in the commercial farming business, which allows
extensive production without the product spoiling due to insects and other factors. Although,
the use of the pesticides/chemicals has had effect on the contamination of water sources and
the health of people and wildlife. This investigation sought out to determine the effects of
pesticides specifically on bacteria and how it can be potential beneficial in reducing
dangerous bacteria or helping to diminish it. Data was obtained by spraying different types of
pesticides on the bacteria E. coli which is usually found on crops. These results were
analyzed by change in colony size and overall abnormal growth in each trial. The results
supported that there was a change in the bacterias colony size between our first and our last
trial.

THE EFFECTS OF PESTICIDES ON E. COLI

12

The effects of pesticides, such as Bayer Advanced Garden Fruit, Citrus and Vegetable
Insect Control, Bayer Natria Insect, Disease and Mite Control, and Ready-to-Use
Multipurpose Garden Insect Killer on bacteria found on fruit and vegetables such as
E.coli.
Introduction
This research project is designed to test how the pesticides/chemicals Bayer
Advanced Garden Fruit, Citrus and Vegetable Insect Control, Bayer Natria Insect,
Disease and Mite Control, and Ready-to-Use Multipurpose Garden Insect Killer effect
commonly found bacteria on fruits and vegetables-E. coli. Pesticides/chemicals are a
major use in the commercial farming business, which allows extensive production
without the product spoiling due to insects and other factors. This allows farmers to save
money on spoil crops and decrease amount of food wasted. The use of the
pesticides/chemicals has had effect on the contamination of water sources and the health
of people and wildlife. This has launched a major cultural debate of using organic
techniques in growing crops to reduce these hazards.
The goal of this research project is to determine if bacteria is affected by
pesticides/chemicals. We will experiment on E.coli with the use of the pesticides Bayer
Advanced Garden Fruit, Citrus and Vegetable Insect Control, Bayer Natria Insect,
Disease and Mite Control, and Ready-to-Use Multipurpose Garden Insect Killer which

THE EFFECTS OF PESTICIDES ON E. COLI

13

are commonly used in gardening and planting. The main objective of this project is to
determine the effects of pesticides on the effect on the bacterias structure, growth, and
behavior. Findings from this project, may figure out if chemicals/pesticides will have a
negative or positive effect in eliminating potential disease causing bacteria found in
major commercial crops.
Should this project find that the effect of pesticides decrease the potential growth
and destroy the structure of the bacterium which would protect consumers from potential
biohazards. Should this project find that the effect of pesticides increase the potential
growth and further allow the bacteria to grow immune to the use of chemical which
would negatively affect human health and increase the chance of widespread diseases by
making the bacterium resistant to chemicals that could have possibly be used to kill them.
Other environmental factors that could be tested that would affect the bacterial growth,
structure, and behavior would be type of soil, temperature conditions where bacteria is
stored or food is grown in, sunlight that the bacteria is exposed in, water that the plant
was used for watering, etc.
Pesticides have been an issue in the consumer market that use it for mass
production of crops. They have included issues such as contaminating water projects and
causing health issues in people and animals that consume it. Bacteria in food have also
been an issue causing sickness such as the E.coli bacteria that plagued fruits and
vegetables through time.
Wilson and Otsuki (2004), concerned with how governments regulate food safety
and environmental protection, along with pesticide residue levels, their paper explores the

THE EFFECTS OF PESTICIDES ON E. COLI

14

food safety standards and whether food pesticide levels have an effect on international
trade flows. In order to begin their research, they examined regulatory trade data from 11
Organization for Economic Cooperation and Development importing countries, along
with trade data from 21 exporting countries, such as Latin America, Asia, and Africa.
Their finally findings suggested that an increase by 1% in regulatory stringency, meaning
tighter restrictions on pesticide chlorpyrifo - ultimately lead to the decrease in banana
imports by 1.63%. Their findings demonstrate a significant impact on trade to countries
that rely on agricultural commodity exports such as bananas. The teams findings
deduced that the lack of international consensus standards and national regulations gone
divergent on pesticides is quite costly.
It has been prominent that the use of chemical inputs, much like common
pesticides has significantly increased agricultural production and productivity; however,
negative effects from this use has increased as well. These effects included damage to the
agricultural land, fisheries, flora, and fauna. Another downfall to the use of such
pesticides is the unintentional destruction of the beneficial predators to pests, which then
in turn increases the livelihood of many species of agricultural pests. In addition, such
externalities threaten and increase the mortality and morbidity of humans due to the
exposure to pesticides is being recorded, especially in developing countries. The cost in
damage from these pesticides is large and affects the farmers returns. However, despite
the large costs that the effects bring, farmers continue to spray pesticides on crops in
increasing quantities. This paper examines the harmful effects of pesticides, along with
the statistical data of farmer use of common pesticides.

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15

The Bacteria E. coli is known to provoke food poisoning in people. These

symptoms include hemorrhagic colitis which is diarrhea with very little stool and large
amounts of blood, this occurs up to three days after eating contaminated food. The ways
of being infected by the E.coli causing food poisoning include undercooked meat such as
hamburgers, unpasteurized apple juice and cider, raw milk, contaminated water or ice,
vegetables fertilized by cow manure, or spread from person to person. Other ways of
catching the bacteria include intake of raw vegetables or cool, moist foods (such as potato
and egg salads) that are handled after cooking. People who are infants and the elderly are
more prone to getting food poisoning, having sickle cell anemia, taking antibiotics and
other medicines, having pre-existing medical condition, and people who have weakened
immune system such as pregnant women also increase the risk of getting food poisoning.
(UMMC, 2013)
Some organochlorine pesticides prevent the growth of nitrogen-fixing bacteria
from restoring natural nitrogen fertilizer in soil, resulting in lower crop harvest and
growth. This has resulted in the need of for more chemical additives to boost production.
This effect is caused from pesticides interfering with flavonoid directing from the family
of leguminous plants like alfalfa, peas, and soybeans to soil bacteria that fix nitrogen.
People assume that endocrine disruption by pesticides occurs only in humans and
animals with estrogen receptors, but we find there are nontraditional targets affected by
pesticides, says Jennifer Fox, a researcher at the University of Oregon for the Center for
Ecology and Evolutionary Biology ( Potera, C. 2007).

THE EFFECTS OF PESTICIDES ON E. COLI

16

Studies from researchers Savino MJ1, Snchez LA, Saguir FM, de Nadra MC.
found that lactic acid bacteria (LAB) secluded from two apples, use arginine at different
initial pH values. The apples exterior contained an average level of bacteria ranging from
log 2.49 0.53 to log 3.73 0.48 cfu/ml . Thirty-one strains were able to develop in
presence of arginine at low pH. They were phenotypically and genotypically classified as
Lactobacillus, Pediococcus and Leuconostoc division. Overall, they did not provoke the
presence of ammonia from arginine when cultured in pH level 4.5 or 5.2 in basal medium
with arginine.(Savino MJ1, Snchez LA, Saguir FM, de Nadra MC. ,2012).
In Germany in May, June, and July, 2011 a study was conducted to describe an
outbreak of gastroenteritis and the hemolyticuremic syndrome that is= caused by Shigatoxinproducing Escherichia coli Sprouts were identified as the leading cause of the
infection, since it carried the bacteria. The data came from reports in Germany of Shigatoxinproducing E. coli gastroenteritis and hemolyticuremic syndrome. Clinical
information on the people affected by the syndrome was given to Hamburg University
Medical Center (HUMC) to study the cause of it. An outbreak case occurred of the
hemolyticuremic syndrome or of gastroenteritis in patients infected by Shiga-toxin
producing E. coli. This outbreak was caused by an abnormal E. coli strain. The cases of
the hemolyticuremic syndrome occurred frequently in adult females. The hemolytic
uremic syndrome occurred in more than 20% of the cases. ( Frank C., 2011).
Materials and Methods
Data began by culturing E.coli bacteria in a petri dish before starting the first trial.
The cultured bacteria was incubated for three days before being used. After the bacteria

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17

was cultured, the first control group was set up to be the single original colony that the
bacteria was going to originate from. This was set up so that the E. coli bacteria would be
similar and prevent confounding variables of abnormalities due growing from a different
source. This was also set up so we could compare the final petri dishes to the original
parent and see if any abnormalities in growth occurred from the generation of the first
trials to the generations of the last trial. The parent petri dish was set in a constant
incubation of 24.5 degree Celsius for four days. This temperature was selected because it
is the average temperature environment for annual crops in U.S. This was set up to
simulate the environment as close as possible to that of a crop field and the usual
temperature that these bacteria would form on crops. A confounding variable that differs
from the usual environment is that the bacteria was grown in agar rather than on fruit or
vegetable.
After the bacteria was cultured, the first trial was set up. The first trial began with 4
petri dishes labeled A,B,C,and, D. The bacteria from the parent dish were looped and spread
out through all four of the petri dishes. Petri dish A was to be treated with the pesticide Bayer

Fruit, Citrus, Vegetable Insect Control pesticide, and spray the bacteria, dish B sprayed
with Bayer Natria Insect, Disease and Mite Control RTU, 24 oz. pesticide, dish C with 24
oz. Ready-to-Use Multipurpose Garden Insect Killer pesticide, and dish D with no
treatment. The three dishes that were treated with the pesticides were sprayed three times
to ensure that the entire dish was covered. After the dishes were sprayed, they were
parafilm and incubated as the same temperature as the parent dish. The dishes were
incubated for eight days. After the eight day, we began collecting data from the first trial
and setting up the second trial to incubate. To collect data we began recording the size of

THE EFFECTS OF PESTICIDES ON E. COLI

18

the largest colony from each dish. We then gram stained a sample from each dish and
observed it under a microscope. The data was then recorded in our notebooks. After our
observations we began preparing the second trial to incubate. The bacteria from the parent
dishes A, B, C, and D were looped and spread out through their second trial; petri dishes,
such as A2 being looped and spread from bacteria from dish A. Petri dish A2 was to be
treated with the pesticide Bayer Fruit, Citrus, Vegetable Insect Control pesticide, and spray

the bacteria, dish B2 sprayed with Bayer Natria Insect, Disease and Mite Control RTU,
24 oz. pesticide, dish C2 with 24 oz. Ready-to-Use Multipurpose Garden Insect Killer
pesticide, and dish D2 with no treatment. The three dishes that were treated with the
pesticides were sprayed three times to ensure that the entire dish was covered. After the
dishes were sprayed, they were parafilm and incubated as the same temperature as the
parent dish. The dishes were incubated for eight days. The dishes were incubated for
eight days. After the eight day, we began collecting data from the second trial and setting
up the third trial to incubate. To collect data we began recording the size of the largest
colony from each dish. We then gram stained a sample from each dish and observed it
under a microscope. The data was then recorded in our notebooks. After our observations
we began preparing the second trial to incubate. The bacteria from the parent dishes A2,
B2, C2, and D2 were looped and we spread out through their second trial; petri dishes, such
as A3 being looped and we spread from bacteria from dish A2. Petri dish A3 was to be treated
with the pesticide Bayer Fruit, Citrus, Vegetable Insect Control pesticide, and spray the

bacteria, dish B2 sprayed with Bayer Natria Insect, Disease and Mite Control RTU, 24
oz. pesticide, dish C3 with 24 oz. Ready-to-Use Multipurpose Garden Insect Killer
pesticide, and dish D3 with no treatment. The three dishes that were treated with the

THE EFFECTS OF PESTICIDES ON E. COLI

19

pesticides were sprayed three times to ensure that the entire dish was covered. After the
dishes were sprayed, they were parafilm and incubated as the same temperature as the
parent dish. The dishes were incubated for eight days. The dishes were incubated for
eight days.
After the eight day, we began collecting data from the third trial and setting up
the fourth trial to incubate. To collect data we began recording the size of the largest
colony from each dish. We then gram stained a sample from each dish and observed it
under a microscope. The data was then recorded in our notebooks. After our observations
we began preparing the second trial to incubate. The bacteria from the parent dishes A3,
B3, C3, and D3 were looped and we spread out through their second trial; petri dishes, such
as A4 being looped and we spread from bacteria from dish A3. Petri dish A4 was to be treated
with the pesticide Bayer Fruit, Citrus, Vegetable Insect Control pesticide, and spray the

bacteria, dish B2 sprayed with Bayer Natria Insect, Disease and Mite Control RTU, 24
oz. pesticide, dish C4 with 24 oz. Ready-to-Use Multipurpose Garden Insect Killer
pesticide, and dish D4 with no treatment. The three dishes that were treated with the
pesticides were sprayed three times to ensure that the entire dish was covered. After the
dishes were sprayed, they were parafilm and incubated as the same temperature as the
parent dish. The dishes were incubated for eight days. The dishes were incubated for
eight days. After the eight day, we began collecting data from the fourth trial. To collect
data we began recording the size of the largest colony from each dish. We then gram
stained a sample from each dish and observed it under a microscope. The data was then
recorded in our notebooks. After our observations we ended our data collection by killing

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the bacteria in the dishes with bleach and disposing of them. The data that was collected
were analyzed and incorporated in a chart and graph of the change.
Results/Discussion
For the results of our project, we used qualitative and quantitative methods of
collecting data. Our quantitative analyses consisted of, measuring the size of an
individual colony within each Petri dish per trial; it was using this technique that we were
able to note the small and large growth changes in the colonies between each trial as
shown in Figure 1. The following is an accurate rundown of what those findings were:
starting with Dish A (Bayer Fruit, Citrus, Vegetable Insect Control), we noticed that
growth change between the first two trials was very minimal (only a 0.1 mm increased
difference) but the changes among three and four were pretty significant, the growth
change between these two marked a whole 1.0 mm growth increase, which lead us to our
final conclusions on Dish A, that being that it was the only dish that had a 1.6 mm growth
increase between the first and last trials. In Dish B (Bayer Natria Insect, Disease, and
Mite Control), we observed what was turning out to be a promising growth increase
begin slowly decreasing with each new trial. Dish Bs representative colony started out at
0.7 mm, and by trial two, had already dropped 0.2 mm; this pattern was also noted with
trials three and four, which had lost the same amount, that leaving trial five, which had
only decreased by 0.1 mm. By the end of all four trials, we noted that only Dish B had
decreased by 0.6 mm between the first and last trials. These results were compared in our
graphs as seen in figure 2.

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Moving onto Dish C ( Ready-To-Use Multi Purpose Garden Insect Killer), after
running all the numbers, we had realized that this dish had the greatest colony size
growth through all four trials in comparison to every other dish. Right out of the gate, the
colony had started at 0.3 mm and by trial two had grown by 0.2 mm, that value jumping
to a 1.0 increase in trial three. Within the last trial, we observed an even bigger increase
in growth, 1.5 mm; an additional component we marked on for Dish C was that although
it had the largest colony sizes, it also had the least amount of colonies altogether. Dish D
(our control) had stayed constant throughout all four trials, which is what we wanted so
we could compare our pesticide tested dishes to it. The dish managed to stay
uncontaminated by lose pesticide residue and by being in close proximity to the tested
dishes, which is fortunate otherwise we may have had an outlier variable within the
results.
As far as the qualitative observations go, they are slightly more limited than the
quantitative data findings due to the fact that we werent able to analyze the physical
samples in depth, not only did we not have the time but we didnt have access to those
advanced tools in order to run such observations. However, we were able to gather other
significant observations, all of which were allotted to each of the dishes. To begin, in
Dish C, we found it to have the largest overall colony size growth through all four trials,
but had also noted that right from the start, it had the least amount of colonies to work
with, what this dish had gained in colony size, it had lost in colony amount. We had
found this to be rather odd and hypothesized that it could have been due to the colonies
lumping together to form larger ones. Moving on, Dishes A and B had the most amount

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of colonies sprayed across the surface of the whole plate, thus making them the only two
that had developed a lawn of bacteria. While observing both dishes extensively, we
noticed that the colonies in those dishes from trial one to four had dramatically swelled in
size. These dishes had started out thinly spread across the surface of the plate and small in
size in trial one but by trial three, had become severely lumped together and enormous in
size. Last but not least, Dish D managed to stay constant and controlled through all four
trials as we intended it to be. We also noted that on the three pesticide tested dishes, a
strange jelly-like substance had begun to form on the surface of them as seen in Figure 3;
we dont know what the substance as we didnt have enough time to test it but we can
only deduce it was due to the pesticides.
Conclusion and Future Work
By the end of this project, my partner and I had rejected our null hypothesis but
had accepted the alternative hypothesis. All in all, We concluded that the bacteria, E.
colis growth was affected after applying the pesticides/chemicals Bayer Advanced
Garden Fruit, Citrus and Vegetable Insect Control, Bayer Natria Insect, Disease and Mite
Control, and Ready-to-Use Multi Purpose Garden Insect Killer. After looking over our
dishes once more and double checking our calculations, we found that there was in fact a
distinct difference in the dishes that had been sprayed with pesticide in comparison to the
control we had used throughout the course of the experiment. The results of this
experiment go either way as far as determining if the pesticides had caused the bacteria to
shrink or grow, which is why we went with a nondirectional alternative hypothesis that

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23

matched with what we were predicting. We were specifically looking for change, which
is what we got with every tested dish used within the duration of our experiment.
Unfortunately, due to the lack of time available, and resources at our disposal, we
weren't able to assess in what other ways the bacteria had been affected by the pesticides;
the only physical and chemical component we were able to dissect in full was the size of
colonies within all three dishes. If both of those factors had been unlimited, we couldve
gone into deeper analyses, such as checking the chemical composition of the bacteria
from its original state to its apparent mutated form after the trials. We could have run
more trials to determine if the pesticides actually caused the bacteria to shrink and die
away or if the created mutant forms of it that were able to thrive under the harsh
conditions that the pesticides presented. In addition to running more tests, we couldve
test for DNA analysis between the original strain of bacteria to its end product. This test
wouldve been able to determine for us if the DNA of the bacteria had been genetically
altered by its used pesticide.
Although we did obtain the results we were hoping for, there were a few obstacles
to overcome along the way, and that no doubt had a hand in affecting our results; all of
which we had little to no control over. For starters, we found that after gram staining our
slides, we were unable to view the results under the highest level of magnification using
oil immersion; we were capable of focusing the subject on every other level, but once we
got to this one, what we were viewing was either extremely blurred (enough to be unable
to take significant observations away), or we couldnt find it at all. Even when we had
achieved the highest magnification possible for ourselves, what we saw was still too

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24

small to interpret change and draw a scale bar to determine the size of each individual
bacteria. In addition to this, there were too many colonies to count and use as
measurements for our hypothesis; therefore, we had to resort to measure the size of the
largest colony of each dish as data. There was multiple issues with this method as even
though Dish C had the biggest colonies, it had the least amount of them in all four trials.
There was also the issue that arose when the jelly-like substance had begun growing on
Dish A and B, were still not sure if this was due to the pesticides interacting with the
bacteria and/or the nutrient agar, or if it was due to error on our own part, i.e.
contamination; regardless, it was most certainly an outlier variable that needed to be
addressed properly.
In conclusion, if we were given the chance to expand upon our work in the near
future, we would definitely take bigger precautions into our methodology techniques.
Starting with our tools, we could obtain and work with a stronger microscope in order to
see if individual bacterium had been affected by the pesticides, and then would compare
how the same strain of bacteria had been affected by different pesticides. Using a stronger
microscope would also give us the ability to observe all of the nitty-gritty ways the
bacteria had changed, we would be able to properly assess its changed form and structure.
In addition, we would broaden the types of bacteria to be tested on by swabbing different
fruits and vegetables from the greenhouse at school. Changing this component of our
design would give our project a little more diversity, as we only tested one type of
bacteria commonly found on typical supermarket produce.

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References
Frank, C. (2011, November 16). Epidemic Profile of Shiga-ToxinProducing Escherichia
coli O104:H4 Outbreak in Germany NEJM. Retrieved October 6, 2014, from
http://www.nejm.org/doi/full/10.1056/NEJMoa1106483
Food poisoning. (2013, January 1). Retrieved October 6, 2014, from
http://umm.edu/health/medical/altmed/condition/food-poisoning

THE EFFECTS OF PESTICIDES ON E. COLI

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Savino MJ1, Snchez LA, Saguir FM, de Nadra MC. (2012, March 23). Lactic acid
bacteria isolated from apples are able to catabolise arginine. Retrieved October 6,
2014, from http://www.ncbi.nlm.nih.gov/pubmed/22805821
Wilson, J., & Otsuki, T. (n.d.).(2004) To Spray Or Not To Spray: Pesticides, Banana
Exports, And Food Safety. Food Policy, 131-146.
Wilson, C., & Tisdell, C. (n.d.). Why farmers continue to use pesticides despite
environmental, health and sustainability costs. Ecological Economics, 449-462.
Potera, C. (2007, May 28). Agriculture: Pesticides Disrupt Nitrogen Fixation. Retrieved
October 6, 2014, from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2137115/

THE EFFECTS OF PESTICIDES ON E. COLI

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Figure 1. This figure shows the data collection of the size of the largest E. coli colonies in
each petri dishes.

Figure 2. This figure shows the correlation between pesticide trials and size of E. coli
colonies and comparison between all four dishes through the four trials.

THE EFFECTS OF PESTICIDES ON E. COLI

28

Figure 3. This figure shows the change in the E. colis consistency and texture from the
third trial compared to the first in dish trial A to A3.

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VITA
Biomedical Science program
West Career and Technical Academy
Hunter Galindo
Skills
Skilled in genotyping
ELISA assay
Gene expression
Organization of Biological Field Stations
Separation and purification techniques
Medical research comprehension
Diabetes and metabolism expertise
Retouching and color correction
Black and white darkrooms
Wildlife photography
Education
Veterinary Sciences Program
West Career and Technical Academy: 11945 W Charleston Blvd, Las Vegas, NV
89135
Program Courses:

Principles of Biomedical Sciences Human Body Systems

Zoology

Medical Interventions

Biomedical Innovations

AP Courses:

AP Chemistry

AP Biology

THE EFFECTS OF PESTICIDES ON E. COLI

30

Computer Skills:

Computer programs (Apple)/websites proficient in

Grapher

iMovie

Garage Band

Prezi

Glogster

Storybird

Weebly website creation

Wix website creation

Academic Achievements:

9th-12th grade: perfect attendance

9th-10th grade: honor roll

Other Academic Information:

Current GPA: 3.7

Class Rank: 201 of 325

SAT overall score: 1290

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VITA
Biomedical Science program
West Career and Technical Academy
Silva Topchyan
Scholastic Information
West Career and Technical Academy
(Current GPA-weighted: 4.211, unweighted: 3.411, Class Rank: 107 of 306)
Advanced Honors Diploma anticipated June 2015
Attending the University of Nevada, Las Vegas for biomedical sciences;
concentration in pre professional in Fall 2015
Academic achievements:
Completing High School to health care program 2014
National Workplace Readiness skills Certification(2013-2014 Assessment)
National Workplace Readiness skills Certification(End of program Assessment
Biomedical)
Club involvement:
National Junior Honor society: 2009-2011
Biomedical Field Station: 2012-2014
Workshop:
High School to Health Care program 2014 (Southern Hills Hospital)
Senior Research Project (Biomedical program)
Redrock interpretation exhibit on Entomology and Botany
Volunteer work:
Tutoring- 2009-2013
Three Squares- 2010
Sahara West Library volunteer: 2012-2013
Red Rock interpretation program: 2012-2014