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Knowledge Check
Scientific Method
Scenario
Summary
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Scientific Method
Scenario
1.
Formulate
a
hypothesis
2.
Propose
an
experiment
to
test
the
hypothesis
3.
Interpret
results
of
the
experiment
Summary
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By
chance
Asymmetric
cell
division
Cell-cell
signaling
Knowledge Check
Scientific Method
Scenario
Summary
By
chance:
The
two
daughters
will
inherit
identical
DNA.
But
it
is
unlikely
that
they
will
receive
EXACTLY
the
same
number
of
molecules
of
a
particular
mRNA
or
protein
even
if
they
are
randomly
distributed
within
the
mother
cell's
cytoplasm.
For
example,
if
there
are
only
a
few
molecules
of
an
important
protein
X
in
mom
-
say
3
-
then
inevitably
one
daughter
will
receive
at
least
twice
as
much
protein
X
as
the
other.
We
already
learned
that
this
initial
dierence
can
then
be
amplied
by
feedback.
If
protein
X
regulates
gene
expression
then
the
two
daughters
will
end
up
expressing
dierent
genes.
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Cell-cell
signaling:
Either
the
mother
cell
receives
an
asymmetrical
signal
from
an
adjacent
cell
or
only
one
of
the
daughter
cells
receives
a
signal
from
an
adjacent
cell.
A
signal
to
the
mother
cell
can
cause
her
to
localize
specic
mRNAs
or
proteins
asymmetrically
within
her
cytoplasm
-
placing
them
either
near
or
away
from
the
source
of
the
signal.
If
she
then
divides
asymmetrically
then
her
daughters
will
be
dierent
(see
mechanism
2).
A
signal
to
only
one
of
the
daughter
cells
can
cause
it
to
change
the
genes
it
expresses.
The
other
daughter,
having
not
received
the
signal,
will
not
make
these
changes
in
the
genes
it
expresses.
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Scientific Method
Scenario
Summary
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Objectives
Mechanisms
Scientific Method
Scenario
Summary
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From
ve
dierent
individuals,
you
isolate
cells
A
&
B
away
from
cells
1-4.
And
then,
just
BEFORE
cell
A
divides,
you
isolate
cell
A
away
from
cell
B.
In
each
case
you
observe:
Scenario
Summary
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Objectives
Mechanisms
Scientific Method
Scenario
Summary
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Mechanisms
Knowledge Check
Scientific Method
Summary
You
conclude
(correctly!)
that
P
must
already
be
asymmetric
when
you
isolate
it
and
that
it
then
divides
asymmetrically,
but
you
want
to
understand
why
cell
R
expresses
TF52
but
its
sister
cell
S
does
not.
Next
Back
Choose
a
hypothesis:
1.
2.
Next
R
Experiment
III.
Isolate
cell
R
immediately
before
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Experiment
V.
Isolate
cells
R
and
S
immediately
after
cell
W
divides.
Observe
which
cell(s)
express
TF52.
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R
I
R
I
Experiment
II.
Isolate
cell
Q
immediately
before
it
divides.
Observe
which
cell(s)
express
TF52.
Experiment
IV.
Isolate
cells
R
and
S
immediately
before
cell
W
divides.
Observe
which
cell
(s)
express
TF52.
R
I
Next
R
Experiment
III.
Isolate
cell
R
immediately
before
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Experiment
V.
Isolate
cells
R
and
S
immediately
after
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Back
R
I
R
I
Experiment
II.
Isolate
cell
Q
immediately
before
it
divides.
Observe
which
cell(s)
express
TF52.
Experiment
IV.
Isolate
cells
R
and
S
immediately
before
cell
W
divides.
Observe
which
cell
(s)
express
TF52.
R
I
Next
R
Experiment
III.
Isolate
cell
R
immediately
before
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Experiment
V.
Isolate
cells
R
and
S
immediately
after
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Back
R
I
R
I
Experiment
II.
Isolate
cell
Q
immediately
before
it
divides.
Observe
which
cell(s)
express
TF52.
Experiment
IV.
Isolate
cells
R
and
S
immediately
before
cell
W
divides.
Observe
which
cell
(s)
express
TF52.
R
I
Next
R
Experiment
III.
Isolate
cell
R
immediately
before
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Experiment
V.
Isolate
cells
R
and
S
immediately
after
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Back
R
I
R
I
Experiment
II.
Isolate
cell
Q
immediately
before
it
divides.
Observe
which
cell(s)
express
TF52.
Experiment
IV.
Isolate
cells
R
and
S
immediately
before
cell
W
divides.
Observe
which
cell
(s)
express
TF52.
R
I
Next
R
Experiment
III.
Isolate
cell
R
immediately
before
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Experiment
V.
Isolate
cells
R
and
S
immediately
after
cell
W
divides.
Observe
which
cell(s)
express
TF52.
Back
R
I
R
I
Experiment
II.
Isolate
cell
Q
immediately
before
it
divides.
Observe
which
cell(s)
express
TF52.
Experiment
IV.
Isolate
cells
R
and
S
immediately
before
cell
W
divides.
Observe
which
cell
(s)
express
TF52.
R
I
Next
Mechanisms
Knowledge Check
Scientific Method
Scenario
There
are
three
mechanisms
by
which
daughter
cells
can
express
dierent
genes:
by
chance,
asymmetric
cell
division,
and
cell-cell
signaling.
By
using
the
scientic
method,
you
can
gure
out
which
mechanism
is
being
used.
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