Results

1. Colonial Macroscopic Examination

Table 2.1 : Colonial Macroscopic Examination

Yeast

Colour

Colonial macroscopic
Texture
Smell

Distribution

White

Slimy

Pungent

Even

Clear White

Creamy

Pungent

Colony

Creamy Red

Moist

Pungent

Line

Pichia spp

Hanselula
capsulata

Rhodotorula
gracilis

2. Microscopic Examination
Table 2.2 : Microscopic examination

Yeasts
Pichia spp
Hansenula capsulata
Rhodotorula gracilis
\

shape
Round
Round
Oval

Microscopic
budding cells
Present
Present
Present

acrospore
Absent
Present
Present

Illustration and Labelled ( Table 2.2 )

a) Pichia spp

Pichia spp Sample

b) Hanselula capsulate spp

Microscope Image

Illustration

Hanselula
Sample

Capsulata

Microscope Image

Illustration

Microscope Image

Illustration

c) Rhodotorula gracillis

Rhodotorula
Sample

3. Sugar Fermentation

gracillis

Table 2.3: Acid production from sugar fermentation test

Yeast

Glucose

Acid production from sugar

Lactose
Maltose

Sucrose

++

+++

+++

Pichia spp

Hanselula
capsulata

++

++

Rhodotorula
++
+++
gracilis
* +++ strong acid production
++ mild acid production
+ low acid production

++

++

+++

+++

Table 2.4: Gas production from sugar fermentation test

Glucose

Yeast

Gas production from sugar

Lactose
Maltose

Sucrose

Pichia spp

Hanselula
capsulata

Rhodotorula
gracilis

* Observe any carbon dioxide gas in durham tube

4. Isolation of yeast from food sample

My food sample : Tempe and Tapai

Table 2.5 : Colonial Macroscopic Examination from food sample

Yeast

Colour

Colonial macroscopic
Texture
Smell

Distribution

White

Moist

Pungent

Dotted

White

Soft furry

Pungent

Colony

Tempe

Tapai

Yeasts

Microscopic Examination
Shape

budding cells

acrospore

Tempe
round
Tapai

present

present

present

oval

llustration and Labelled ( Table 2.5 )

a) Tempe GYEA

present

Microscope Image

b) Tempe PDA

Illustration

Microscope Image

Illustration

b) Tapai GYEA

Microscope Image
b) Tapai PDA

Illustration

Microscope Image

Illustration