Gayam, Glydenne Glaire P.

BS Medical Technology 3A

Bacte MWF 5:30-8:30PM

August 24, 2015

Duration of Incubation and Frequency of Examination of Cultures

Type of Culture
* Plates

Blood
Fluids
Abscesses, wounds
Tissue
Respiratory tract
Throat
Sputum
Transtracheal aspirate
Bronchial washings
Genitourinary
Cervix, Vagina
Uterus, Cul-de-sac
Prostate
Urethra
Urine
Fecal Material
Anaerobic
Brucella
Actinomyces

Incubation Time
14
7*
7*
7*

Frequency of Examination
Daily for 7 days and after 14 days
Daily
Daily
Daily

2
1
7*
7*

Daily
Once
Daily
Daily

2
7*
2
2
1
1
7*
30
21

Daily
Daily
Daily
Daily
Once
Once
Daily
3 x weekly
3 x weekly

are

discarded after 48 hours, but tubes with supplemented fluid thioglycollate are kept for time specified in column.

Initial cultures and subcultures of enrichment broth are examined after 18 to 24 hours for presence of lactose-negative colonies; if
none are present, the cultures are reported as negative for salmonella and shigella.
Todd; Sanford; Davidsohn (). Clinical Diagnosis and Management by Laboratory Methods (17th ed).