2)

a)

Theoriginalsequenceprovidedis:

5CTCTCATTACCCACACACGAAGGAAAGCAGGACCTTCATCCCAGTGACCTCAGCTAAGACTG3

Anditscomplementary(cDNA):

3 GAGAGTAATGGGTGTGTGCTTCCTTTCGTCCTGGAAGTAGGGTCACTGGAGTCGATTCTGAC 5

The translated RNA from the original sequence:

5 CUCUCAUUACCCACACACGAAGGAAAGCAGGACCUUCAUCCCAGUGACCUCAGCUAAGACUG 3

The cDNA is translated into cRNA

3 GAGAGUAAUGGGUGUGUGCUUCCUUUCGUCCUGGAAGUAGGGUCACUGGAGUCGAUUCUGAC 5

b)
The protein sequence for the original:
LSLPTHEGKQDLHPSDLSD

For the complementary:

Q S stop L R S L G stop R S C F P S C V G N E

c)
First frame:
5 CUC UCA
CCC
AGU

UUA
GAC

CCC
CUC

ACA
AGC

CAC
UAA

GAA
GGA
GAC3

AAG

CAG

GAC

CUU

CAU

L
P

L
D

P
L

T
S

H
stop

E
D

CCA
UCA

CAC
GCU

ACG
AAG

AAG
GAA
ACU3

AGC

AGG

ACC

UUC

AUC

S
S

Secondframe:

5 UCU CAU
CCA
GUG

UAC
ACC

S
P

H
V

Y
T

P
S

H
A

T
K

K
T

5 CUC AUU
CAG
UGA

ACC
CCU

CAC
CAG

ACA
CUA

CGA
AGA

L
Q

T
P

H
Q

T
L

R
R

AGG
AAA
CUG 3

GCA

GGA

CCU

UCA

UCC

Third frame:

I
stop

Andthreeframesfromthecomplementary:

Fourth frame:
5CAG UCU
CCU
UCG

UAG
UGU

CUG
GUG

AGG
GGU

UCA
AAU

CUG
GGA
GAG3

UGA

AGG

UCC

UGC

UUU

Q
P

stop
C

L
V

R
G

S
N

L
E

stop

5AGU CUU
CUU
CGU

AGC
GUG

UGA
UGG

GGU
GUA

CAC
AUG

UGG
AG3

GAU

GAA

GGU

CCU

GCU

UUC

S
L

S
V

stop
W

G
V

H
M

W
R

5GUC UUA
UUC
GUG

GCU
UGU

GAG
GGG

GUC
UAA

ACU
UGA

GGG
AUG
GAG3

AAG

GUC

CUG

CUU

UCC

V
F

A
C

E
G

V
stop

T
stop

G
E

S
S

Fifth frame:

L
R

Sixth frame:

L
V

As can be seen in the resulting 6 frames, only the second does not have a stop and
hence is the correct one.