Sunteți pe pagina 1din 3

Synevohttp://www.synevo.

ro

Acid1,4metilimidazolacetic(metabolithistamina)
PostedBySynevoOnaugust19,2009@4:34pmInAlergologie,Testepentrumonitorizareainflamatieialergice|NoComments

Informatiigenerale
Histamina,oaminabiogenavasoactiva,constituieprobabilcelmaicunoscutmediatorstocatingranulelemastocitelorsibazofilelor
curolcentralinraspunsulalergic.Laommastocitelecontinaproximativ25pghistamina/celula,intimpcebazofileleprezintao
cantitatemairedusa(1pg/celula).
Mediatoriimastocitari,inclusivhistamina,predominainrapunsulalergicprecocebazofilelereprezintasursamajoradehistaminain
raspunsulalergicdefazatardiva2.
HistaminaestesintetizatainaparatulGolgiprindecarboxilareaaminoaciduluihistidinainprezentaenzimeihistidindecarboxilaza.
Histaminaareosarcinaelectricapozitivasiseasociazacugruparilecarboxilaleproteoglicanilordingranulelemastocitelor.Dupa
exocitoza,histaminadisociazadeproteoglicanilaunpHneutru.Odataeliberatainspatiulextracelular,histaminaestemetabolizata
rapid(timpdeinjumatatire~1520minute)prinmetilaresioxidare.Metilarea,catalizatadeenzimahistaminmetiltransferaza,
constituieprincipalacaledemetabolizarelanivelulcailorrespiratorii.Produsulinitial,Nmetilhistamina,poatefiexcretatprinrinichi
saupoatefideaminatsubactiuneamonoaminoxidazeirezultandacidul1,4metilimidazolacetic(MIIA).Histaminamaipoatefi
oxidatasidecatrediaminoxidaza(histaminaza)cugenerareaaciduluiimidazolacetic(IAA).
Datoritametabolismuluirapidestedificilsasedeterminehistaminainplasmainschimbmasurareahistamineisiametabolitilorsai
inurinadin24oreconstituieoalternativaeficientapentrutestarecaurmareafaptuluicavalorileacestorasementinmultmaimult
timpcrescute1.
MIIAesteprincipalulmetabolitalhistaminei(50%dinhistaminaestedegradatainacestcompus)dinacestmotivreprezintaun
markercantitativpentrueliberareaendogenadehistamina7.Astfel,MIIApoatefiutilinconfirmareadiagnosticuluideanafilaxie6.De

asemeneasaconstatatcanivelurileurinaredeacidmetilimidazolaceticsecoreleazastranscuseveritateamastocitozeisistemicesi
potconstituiunintrumentutilatatpentruconfirmareadiagnosticuluicatsipentruapreciereaevolutieisiaprognosticului3.
Uneleformeraredetumoricarcinoidegastricesemanifestaclinicprintruntablouatipicmediatprinhistaminaeliberatadecatre
celuleletumoralelaacestipacientideterminareadeacidmetilimidazolaceticpoatefiutila,impreunacuceadegastrinasiacid5
hidroxiindolacetic5.
Recomandaripentrudeterminareaacidului1,4metilimidazolaceticevaluareapacientilorcususpiciunedeanafilaxiesau
demastocitozasistemica36.
Pregatirepacientsevaevita,cu2zileinaintearecoltarii,consumuldealimentecuuncontinutridicatinhistamina1.
Recipientderecoltarevasde23litrisipahardeplasticdeunicafolosintapentruurina,pecaresenoteazacantitateatotalade
urinadin24ore4.
Cantitaterecoltata10mL4.
Cauzederespingereaprobeispecimencarenuafostpastratla28Cutilizareadesubstanteconservante(produc
interferente)4.
Prelucrarenecesaraduparecoltareinesantionulrecoltatsevoradaugacatevapicaturideacidaceticglacial4.
Stabilitateproba5zilela28C1lunala20C4.
Metodacromatografiedelichidecuplatacuspectrometriedemasa(LC/MS)1.
Valoridereferinta<3.8mg/24h4.
Limitesiinterferente
ObtinereauneivalorinormaleMIIAnuexcludeunevenimentanafilactic6.


Bibliografie
1.FlorenceIdaHsu,JoshuaABoyce.BiologyofMastCellsandtheirMediators.InMiddletonsAllergy,SeventhEdition.,MosbyElsevier,
2009,320.
2.J.AndrewGrant,SaffanaN.Hassan,PatriciaA.Leonard.MastCellandBasophilderivedMediators.InAtlasOfAllergicDiseases,2nd
Edition,CurrentMedicineLLC,19.
3.KarinHartmann,StefanieBBrunsandBeateMHenz.Mastocytosis:ReviewofClinicalandExperimentalAspects.InJournalof
InvestigativeDermatologySymposiumProceedings(2001)6,143147.
4.LaboratorSynevo.Referintelespecificetehnologieidelucruutilizate2010.RefType:Catalog.
5.LuigiSantacroce.MalignantCarcinoidSyndrome:DifferentialDiagnoses&Workup.emedicine.escape.com.ReferenceType:Internet
Communication.
6.PhilipLLieberman.Anaphylaxis.InMiddletonsAllergy,SeventhEdition.,MosbyElsevier,2009,1041.
7.RobertHamilton,AnneKageySobotka.InVitroDiagnosticTestsofIgEMediatedDiseases.InDiagnosticTestingofAllergicDiseases,
MarcelDekkerInc,2000,99100.

ArticleprintedfromSynevo:http://www.synevo.ro
URLtoarticle:http://www.synevo.ro/acid114metilimidazolaceticmetabolithistamina/
Copyright2009Synevo.Toatedrepturilerezervate.