Dr. Sushil Kumar Tomar, PhD.

Contact information:
Phone: 8979730563 Email: suzeal@gmail.com, sktomar@outlook.com
Webpage: www.sktomar.weebly.com

Education
PhD in Molecular and Structural Biology (Year: 2010)
Department of Biological Sciences and Bioengineering,
Indian Institute of Technology (IIT), Kanpur, India

Bachelor in Pharmacy (Year: 2004)

Rajiv Gandhi University of Health Science, Karnataka, India

Research Experience
Post-doctoral Research Associate (May 2011- July 2015)
RNA Center, Department of Microbiology, Ohio State University, Columbus, USA
Project Scientist (September 2010- April 2011)
Department of Biological Sciences & Bioengineering, IIT Kanpur, India

Specialization
Molecular Biology, Biochemistry, Recombinant DNA Technology, Immunology.

Publications

{Click for Google Scholar profile}

Total Impact Factor: 69.32, Total Citations: 84

Soneya Majumdar, Abhishek Acharya, Sushil Kumar Tomar and Balaji Prakash.
Disrupting domain-domain interactions is indispensable for EngA-ribosome
interactions.The FEBS Journal. (Submitted).

Irina Artsimovitch, Zhaukun Liu, M NandyMazumdar and Sushil Kumar Tomar.

All it takes to express virulence genes. The FASEB Journal (2014) 28: 361.3.
Abstract

Sushil Kumar Tomar, Stefan H. Knauer, Monali NandyMazumdar, Paul Rsch

and Irina Artsimovitch. Interdomain contacts control folding of transcription factor
RfaH. Nucleic Acids Research. (2013) 41 (22):10077-10085. (Impact Factor: 9.1,
Citations: 11)
Sushil Kumar Tomar and Irina Artsimovitch. NusG proteins-universal tools of
transcription modification and communication. Chem. Rev. (2013) 113 (11) 86048619. (Impact Factor: 45.67, Citations: 13)

Sushil Kumar Tomar, Prashant Kumar, Soneya Majumdar, Varun Bhaskar, Prasun
Dutta and Balaji Prakash. Extended C-terminus and length of the linker connecting
the G-domains are species-specific variations in the EngA family of
GTPases. FEBS Open Bio. 2 (2012) 191-195. (Impact Factor: 1.52, Citations: 1)

Sushil Kumar Tomar, Prashant Kumar and Balaji Prakash. Deciphering the
catalytic machinery in a universally conserved ribosome binding ATPase YchF.
Biochem Biophys Res Commun. (2011) 13; 408(3):459-64. (Impact Factor: 2.281,
Citations: 12)

Sushil Kumar Tomar, Neha Dhimole, Moon Chatterji and Balaji Prakash. Distinct
GTP/GDP bound states of the tandem G-domains of EngA regulate ribosome
binding. Nucleic Acids Research. (2009) 37 (7):2359-2370. (Impact Factor: 9.1,
Citations: 22)

Nikhil Jain, Neha Dhimole, Abu Rafay Khan, Debojyoti De, Sushil Kumar
Tomar, Mathew Sajish, Dipak Dutta, Pradeep Parrack and Balaji Prakash. E.coli
HflX interacts with 50S ribosomal subunits in presence of nucleotides. Biochem.
Biophys. Res.Commun. (2009) 379 (2):201-5. (Impact Factor: 2.281, Citations:
25)

Nonscientific writing
Nature India blog "Away from home: But close to my dream" 19th June 2013.

Award/honors

Travel award from Council of Scientific and Industrial Research, India to attend The
Complex Life of mRNA: From Synthesis to Decay EMBL Heidelberg, Germany March,
2010.

Travel award from EMBO and Department of Science and Technology, India to attend
8th international conference on ribosome Synthesis held in Regensburg, Germany from
August, 2009.

Travel award from Department of Biotechnology, Ministry of Science and Technology,

India to attend 8th international conference on ribosome Synthesis held in Regensburg,
Germany from August, 2009. (Not availed- due to funding from other sources.)

Junior and Senior research Fellowships from Ministry of Human Resources and
Development, Govt. of India for pursuing Ph. D. at IIT Kanpur.

Graduate Aptitude Test in Engineering-2004 (GATE) Percentile 99.42 (All India Rank49)

Graduate Aptitude Test in Engineering-2003 (GATE) Percentile 95.6 (All India Rank276)

First Position in B. Pharmacy 2004 Batch.

Relevant Conferences

Poster presentation titled Elucidating recycling of RfaH at Cleveland (Ohio) USA.

RRM meeting. October 2013.

Oral presentation titled Structural transition as means for regulation in a Transformer

protein RfaH at 16th Biennial Meeting on Post-initiation Activities of RNA
Polymerases. Transcription meeting Pembroke, Virginia, USA, November, 2012.

Poster presentation titled YchF binds pth mRNA; implication for its role in protein
synthesis at conference The Complex Life of mRNA: From Synthesis to Decay EMBL
Heidelberg, Germany, March 2010.

Poster presentation titled An essential GTPase EngA regulates ribosome biogenesis by

achieving distinct nucleotides bound States at the two tandem G-Domain at 8th
international conference on ribosome Synthesis held in Regensburg, Germany, August,
2009.

Technical Expertise
Molecular biology techniques: Cloning, Site directed Mutagenesis, Protein expression in
E.coli and yeast, Gene deletion in bacteria, Protein purification for biochemical and
crystallization purpose, Excellent understanding of AKTA and others protein purification
system, capable of designing new purification protocols and problem solving skills. Protein
refolding, Western Blots, In vitro translation assays, Cell free protein expression and
purification. Preparing deep sequence library using ribosome profiling, Transcription assays,
Primer extension, Ribosome toe print assays, Nuclease dependent foot print assays.
Analytical techniques: Mant fluorescent nucleotide binding assays, colorimetric and radiolabeled nucleotide hydrolysis assays, gel-retardation assays. Stop flow, Tryptophan Induced
Quenching (TrIQ).
Ribosome handling: Isolation of 30S, 50S, 70S Ribosome from E.coli and Bacillus strains,
Factor-ribosome binding assays, Ribosome profiling, ISCO Gradient maker handling.
Software: MS word, office and PowerPoint. Adobe Photoshop, Origin, Graphpad Prism.
Other relevant Skills: Technology transfer, management, Mentorship, Scientific writing.

Personal Information
Date of Birth: August 2, 1981
Gender: Male
Marital Status: Married
Children: 2
Nationality: Indian
Address: Dayanand Nagar, Shamli
Uttar Pradesh India 247776