Numrul total de ore (pe semestru) din

planul de nvmnt

FIA DISCIPLINEI

Denumirea disciplinei

BIOCHIMIE

Anul de studiu I

Semestrul * II

Tipul de evaluare final (E / V / C)

Regimul disciplinei (Ob obligatorie, Op opional, F - facultativ)

Total ore din planul de nvmnt

168

Total ore de studiu

individual

Ob

Numrul de credite

50

Total ore pe
semestru

84

Titularul disciplinei
* Dac disciplina are mai multe semestre de studiu, se completeaz cte o fi pentru
fiecare semestru
Total
C**
S
Facultatea

MEDICINA

Departamentul

Stiinte morfofuncionale

Profilul

Medicina

Specializarea

Medicina

84

42

L
42

** C curs, L activiti de laborator, P proiect

sau lucrri practice

Cunotine minime necesare studenilor pentru parcurgerea noiunilor de curs/stagii/lucrri

practice
Biochime semestrul I, fiziologie, biologie celular

Obiectivele disciplinei
S ofere studentului noiunile necesare pentru a nelege mecanismele moleculare care stau la baza
tuturor proceselor biochimice i fiziologice din organism.
Cunoaterea, nelegerea i explicarea caracteristicilor biochimice ale constituenilor organismelor
vii, necesare abordrii de ctre un medic a dou obiective majore ale tiinelor medicale:
- nelegerea i conservarea sntii;
- nelegerea i tratarea eficient a bolilor.

Programa analitic a cursului

Lipid metabolism

Nr. ore
12

LIPIDS OF PHYSIOLOGIC SIGNIFICANCE

1.1. Introduction
1.2. Biomedical importance
1.3. Classification of Lipids
1.4. Fatty Acids
1.5. Triglycerides ( Triacylglycerols )
1.6. Phospholipids
1.7. Glycolipids ( Glycosphingolipids )
1.8. Steroids
1.9. Lipid peroxidation
1.10. Amphipathic Lipids
DIGESTION AND ABSORPTION OF LIPIDS
2.1. Digestion of Lipiods
2.2. Absorption of Lipids
METABOLISM OF LIPIDS
3.1. Introduction
3.2. Fatty Acids synthesis
3.3. Mobilization of stored fats and oxidation of Fatty Acids
3.4. Beta-Oxidation of Fatty Acids
3.5. Specialized Fatty Acids: Prostaglandins and related
compounds
3.6. Metabolism of unsaturated Fatty Acids
3.7. Metabolism of Acyl Glycerols
3.8. Metabolism of Phospholipids
3.9. Metabolism of Glycolipids
3.10. Clinical aspects
3.11. Ketone bodies
3.12. Metabolism of Cholesterol
3.13. Plasma Lipoproteins
Proteic metabolism

12

4.1. Introduction
4.2. Digestion of dietary Proteins
4.3. Removal of nitrogen from Aminoacids
4.4. Urea cycle
4.5. Clinical significance of Urea
4.6. Glucose alanine cycle
4.7. Glutamine formation and functions
4.8. Decarboxylation reaction and biogenic amines
4.9. Metabolism of individual Aminoacids
4.10. Metabolism of other Aminoacids
4.11. Metabolism of creatine
METABOLISM OF HEME
5.1. Synthesis of Porphyrins
5.2. Porphyrias
5.3. Degradation of Heme
5.4. Jaundice
Metabolism of purine and pyrimidine nucleotides

6.1. Introduction
6.2. Biosynthesis of Purine Nucleotides
6.3. Catabolism of Purines
6.4. Metabolism of Pyrimidines
6.5. Catabolism of Pyrimidines
Nucleic acids

7.1. Introduction
7.2. Structure and functions (nitrogen bases, nucleosides, nucleotides, RNA,
DNA, histones)
7.3. Nucleic acids organisation
7.4. Replication
7.5. Transcription
7.6. Translation
7.7. DNA repair
7.8. Pharmacological and clinical corelations
Biochemistry of hormones

8.1. Introduction and definitions

8.2. General mechanisms of signaling
8.3. Hydrosoluble hormones and biochemistry: adrenalin, noradrenalin,
insulin, glucagone, POMC derivatives (shynthesis, mecanism of action, influence of
metabolic pathways).
8.4. Liposoluble hormones and biochemistry: thyroid hormones, steroid
hormones (shynthesis, mecanism of action, influence of metabolic pathways).
Programa analitic lucrri practice/stagii
Glucose determination in serum. Oral glucose tolerance test.

3 ore

Total cholesterol, HDL and LDL-cholesterol determination in serum.

3 ore

Triacylglycerol determination in serum.

3 ore

Ketone bodies: importance and posibilities of measurig in serum and urine. Utility
in clinical diagnosis
Total protein determination in serum. Methods of protein separation. Types of
electrophoresis. Western blot technique.
Ureea determination in serum.

3 ore

Creatinine determination in serum. Creatinine clearence.

3 ore

Total bilirubin, direct and indirect bilirubin determination in serum.

3 ore

Uric acid determination in serum.

3 ore

Calcium and magnesium determination in serum.

3 ore

Free iron determination in serum (sideremia).

3 ore

Biochemical assays for urine.

3 ore

3 ore
3 ore

Competene practice dobndite la finalul anului/semestrului

-

Abiliti n efectuarea practic a unor manevre i tehnici specifice laboratorului clinic de

biochimie (utilizarea pipetelor semiautomate, efectuarea de teste rapide specifice).

La stabilirea notei finale se iau n considerare

Ponderea n notare exprimat n

procente
(Total = 100%)

- rspunsurile la examen / colocviu (evaluarea final):

50%

- rspunsurile finale la lucrrile practice de laborator

35%

- testarea periodic prin lucrri de control

15%

- activitile gen teme / referate / eseuri / traduceri /

proiecte etc.

- alte activiti (PRECIZAI) : colocviu

Descriei modalitatea practic de evaluare final E/V (de exemplu: lucrare scris (descriptiv i
/ sau test gril i / sau probleme etc.), examinare oral, colocviu individual ori n grup, proiect,
etc.)
Teste scrise semestriale, rspunsuri la lucrrile practice
Examen final: examen practic i test gril unic (60 ntrebri cu rspunsuri
multiple) - Da
Estimai timpul total (ore pe semestru) al activitilor de studiu individual pretinse
studentului
(completai cu 0 activitile care nu sunt cerute)
4
1. Descifrarea i studiul notielor de
8. Pregtire prezentri orale
curs
4
2. Studiu dup manual, suport de curs
9. Pregtire examinare final
9
3. Studiul bibliografiei minimale
indicate
4. Documentare suplimentar n
bibliotec
5. Activitate specific de pregtire
SEMINAR i / sau LABORATOR
6. Realizare teme, referate, eseuri,
traduceri, etc.
7. Pregtire lucrri de control

10. Consultaii
2

11. Documentare pe teren

12. Documentare pe Internet

13. Alte activiti ....

1

14. Alte activiti ....

25
TOTAL ore studiu individual (pe semestru)
= 25 minimum

Bibliografie obligatorie :
1. Veronica Dinu, Aurora Popescu, E.Truia Biochimie Medical Mic tratat, Editura
Medical, Bucureti, 2002.
2. Cristiana Filip Enzime i coenzime, Editura PIM Iai, 2007.
3. Jerca Luminia, Ungureanu Didona Biochimia hormonilor, Editura TERRA
NOSTRA, Iai, 2001.
4. Jerca Luminia, Dimitriu Daniela Cristina, Filip Cristiana. Reacii chimice
antioxidante, de excreie i detoxifiere, cu implicarea ficatului. Lucrri practice.
Editura Tehnopres, Iai, 2009.
5. Coninutul cursurilor predate.
Bibliografie suplimentar pentru materia de curs i lucrri practice
1. Pamela C. Champe, R.A. Harvey Biochemistry, 2nd edition, Lippincott - Raven
Publisher, 2010
2. Harpers Biochemistry, 23rd edition, 2003.
3. Devlin T.M. Testbook of Biochemistry, 3rd edition, 2003.
4. Foia Liliana. Corelaii clinice n interpretarea testelor de laborator. Editura
Junimea, Iai, 2010.

Data completrii:

Semntura coordonatorului de activitate didactic

15.10.2013

Conf.dr Didona Ungureanu