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Biochemistry
Learning outcomes
1. Explain the chemical properties of
biomolecules
2. Explain the reaction of biology and
biochemistry processes
3. Practice the correct procedure in
biochemistry
4. Practice the correct basic procedure in
plant and animal physiology
BIOMOLECULES
A biomolecule is any molecule that is present in living
organisms, including large macromolecules such as proteins,
carbohydrates, lipids, and nucleic acids, as well as small
molecules such as primary metabolites, secondary metabolites,
and natural products.
Four major classes of biomolecules & building blocks:
1. Carbohydrate
2. Protein
3. Lipids
4. Nucleic acids
Chemical properties
Chemical properties of molecule are depend on
by specific arrangement of atoms
The specific groups : functional groups
Biomolecules Build up
criteria
Carbohydrate
Protein
Lipids
Acid nucleic
Monomer
Monosaccharide
Example:
Glucose
Galactose
Fructose
Ribose
Amino acid
Fatty acid
glycerol
Nucleotides
Polymer
Starch
Cellulose
Polypeptides
Primary , secondary,
tertiary, quaternary
structure
Dna
Rna
Polymerization (process)
condensation
condensation
Esterification
Condensation
(dehydration)- remove
water molecule
Functional group(s)
Carbonyl group
Functions
https://www.khanacademy.org/science/biolog
y/macromolecules
Peer Instructions
Round
Each group studies one
1 macromolecule
Round
other groups
Explain about their
2niches
Round
3
Assessment
1. https://www.khanacademy.org/science/biology/macromolecules/carbohydrat
es-and-sugars/v/molecular-structure-ofglucose
2. https
://www.khanacademy.org/science/biology/macromolecules/lipids/v/molecular
-structure-of-triglyceridesfats
3. https://www.khanacademy.org/science/biology/macromolecules/nucleic-aci
ds/v/dna-deoxyribonucleic-
Biochemical process
Assembling small molecules into
polymeric macromolecules needs
energy
BIOCHEMICAL PROCESSES
All life processes consist of chemical reaction catalyzed by enzymes
What are the primary functions of metabolism?
1. To acquire and use energy
2. To synthesize molecules needed for cellular structure
& function
3. For growth & development
4. To remove waste & other toxins
carbohydrate
or
*
or
D-glucose
an aldose
an aldohexose
D-fructose
a ketose
a ketohexose
C4
TASK 4:
REDRAW
Glycosidic
bond
Disaccharides
Oligosaccharides
Maltodextrins, fructo-oligo-saccharides
Polysaccharides
Classes of carbohydrate
1. monosaccharide
glucose
simplest sugar
represent aldehydes or ketones of from 3-6
carbons in length.
Glucose "blood sugar"
Fructose "fruit sugar"
Galactose the breakdown of lactose.
Glyceraldehydes the metabolic breakdown
of Glucose.
D-glucose
(ring structure)
2. disaccharide
Classes of
carbohydrate
Classes of
carbohydrate
Glycogen
3. polysaccharide e.g. Starch, Cellulose, Chitin,
a. Starch : 2 polyglucose
1. Amylose
- an unbranched
polyglucose that has linkages at the C1 and C4
- quite soluble in water, but
only represents 20% of the
Starch.
2. Amylopectin
- highly branched with -link at
the C1 and C6
- water insoluble and represents
the other 80% of the starch.
b. Cellulose
Classes of
carbohydrate
Classes of
carbohydrate
c. glycogen
linkage similar to amilopectin BUT the branching is more
numerous
Our bodies make glycogen from the unused carbohydrate
in form granules
stored in the liver and muscle tissue of our bodies
When the body needs energy sources How to obtain the
energy?
glycogen
Classes of
carbohydrate
d. chitin
Similar to cellulose in structure and
function
BUT the unit = N-acetyl--Dglucosamine
chitin has an amide group instead of
a hydroxyl group (alcohol)
protein
A.a. contains :
1. amino group
2. carboxylic acid
group
3. side chain
gp/R group
TASK 5:
REDRAW
TASK 6:
REDRAW
L-Alanine
(Ala / A)
L-Arginine
(Arg / R)
L-Asparagine
(Asn / N)
L-Cysteine
(Cys / C)
L-Aspartic acid
(Asp / D)
Glycine
(Gly/G)
L-Histidine
(His / H)
L-Isoleucine
(Ile / I)
L-Leucine
(Leu / L)
L-Lysine
(Lys / K)
L-Methionine
(Met / M)
L-Phenylalanine
(Phe / F)
L-Proline
(Pro / P)
L-Serine
(Ser / S)
L-Threonine
(Thr / T)
L-Tryptophan
(Trp / W)
L-Tyrosine
(Tyr / Y)
L-Valine
(Val / V)
lipid
O
Alkyl gp
Contains
C & H atoms
RCH2
OH
Carboxyl
gp
TASK 7:
REDRAW
Lipid classes
1. Fatty acids
saturated @ unsaturated
2. Triacylglycerols
Lipid classes
Fatty acid :12-20 C
C
CH2
CH2
CH2
CH2
CH2
H3C
CH2 OH
CH2
CH2
CH2
CH2
Nonpolar hydrophobic tail
Triacylglycerol @ triglyceride
Before a fat can be
oxidized, it must be
hydrolyzed to the anion
acid and glycerol.
Biologically this is done by
lipases.
Chemically base hydrolysis
is called saponification.
Lipid classes
saponification
CH2OH
O
CH2O C R3
CH2OH
O
3 KOH
+
CH
OH
2
CH O C R3
O
O
CH2O C R3
3 KO C R3
K salt of a fatty acid
Lipid classes
3. Phospholipids
structural components of membrane
2 types : phosphoglycerides & sphingomyelins
4. Sphingolipids
Important comp. of animal & plant membranes
Contain long chain amino alcohol: sphingosine (animal) &
phytosphingosine (plants)
5. Isoprenoids
Molecule containing repeating 5C isoprene
Consist of terpenes & steroids
6. Wax esters
Complex mixtures of non polar lipids
Serve as protecting coat
Nucleic acid
e.g. ATP
WEEK
ACTIVITY
Week 9
Proposal discussion
Week 10
Week 11
Project
Week 12
Project
Week 13
Data analyses
Project presentation
preparation
Week 14
Project presentation
Learning outcome
Explain the reaction in Biology and Biochemistry
processes
Selected topics
Photosynthesis
Cardiovascular system
Bacterial metabolism
Notes: http://blnds.co/1zW9gag
photosynthesis
Useful websites
http://photoscience.la.asu.edu/photosyn/education/learn.htm
l
http://www.youtube.com/watch?v=-rsYk4eCKnA
http://www.youtube.com/watch?v=GR2GA7chA_c
http://www.youtube.com/watch?v=yfR36PMWegg
http://www.youtube.com/watch?v=slm6D2VEXYs
http://www.youtube.com/watch?v=EQvTEFCANTM
http://www.youtube.com/watch?v=7ynX_F-SwNY
http://www.youtube.com/watch?v=xp6Zj24h8uA
procedures
http://www.marzkreations.com/Chemistry/Chromatography/Dyes/RF-Values.html
Retention Factors.
The amount that each component of a mixture travels can be
quantified using retention factors (Rf).
ideas
Pair work
http://highered.mcgrawhill.com/sites/0073031216/s
tudent_view0/exercise12/m
ultiple_choice_quiz.html
Cardiovascular system
Useful websites
http://www.youtube.com/watch?
v=H04d3rJCLCE
http://www.youtube.com/watch?v=7XaftdE
_h60
http://www.youtube.com/watch?v=bm65xC
S5ivo
http://www.youtube.com/watch?v=-4kGMIqQ3I
Check
http://highered.mcgrawhill.com/sites/0073031216/student_view
0/exercise11/multiple_choice_quiz.html
ideas
Stress vs BP
Watching movie vs BP
Sound vs BP
Caffeine vs
BP
Lavender vs BP
Gaming vs BP
Solat vs BP
Bacterial Metabolism
ideas
Yogurt from non milk
source
Antimicrobial potential
Bacteria for
environment
Flavored Cheese making
Probiotic food