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CG ! TA | cjc¢ !} Ta 3 TIA ' cleo} cic 1 Glc } Gc ! alr } 1 AT Enymet ft tngmet ot + There are MANY different restriction enzymes. + Each restriction enzyme cuts at a unique DNA sequence. The sequence where the enzyme cuts is the restriction site Restriction site ae DNA SS Restriction enzyme cuts the DNA into fragments a —as © @addition of a DNA fragment Sticky end from another source, cut — using same enzyme two fragments stick ‘together by complementary base-pairing phosphate backbones | INA ligase pastes the sugar- E coli bacterium Pen san ah rig wo fever nent ew pao oraine oi 2 ca pi wth © restriction enzyme ‘DNA y es ‘Cut human cell’s DNA with same enzyme, specific op Se mz Mh ion OQ and plasmid DNA ee) j Se Recombinant ua Gene Recombinant DNA Gene iad ot laren ° Insulin proteinis © putrecombinant plasmid ° elated from the 4 > etetremey ond eects Recombinant => ove bacterium Clone m of ce

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