Microbiology Laboratory

Group # 2
2G-PH
Members: Alodia O. Castillo
Alison A. Claveria
Jehhan N. Decampong
Angelica B. Dela Rosa

April 6, 2016
Paul D. Chiong
Regina DG. De Guzman
Aryssa Gem D. Dela Cruz

I. Identification of Unknown From A Chemical Sample

Observations & Results
Name of the organism: Escherichia coli
A. Plated Media
Description of the colony
1. EMB
2.MacConkey
3.SSA

Interpretation of the results

Colorless

Non-lactose fermenters

Pink

Lactose fermenters

Colorless

Non-lactose fermenters

Before inoculation:

EMB

MacConkey

SSA

MacConkey

SSA

After inoculation:

EMB

B. Tubed Media
Description of the Colony

Interpretation of Results

1. TSI

There is formation of bubbles and

displacement of medium.

A
A with gas production

2. LIA

Purple

K
K (alkaline)

3. SIM

Hydrogen Sulfide (-),

Indole (+)

Motile

4. SCA

Prussian Blue

Positive (+)

5. Trypton Blue (TB)

Red/Fuchsia ring

Positive (+)

6. Methyl Red (MR)

Red

Positive (+)

Yellow

Negative (-)

Salmon/Orange

Negative (-)

7.Voges Proskauer (VP)

8. Urea Broth

Before inoculation:

TSI

After inoculation:

LIA

TSI

LIA

Before inoculation:

SIM

SCA

TB

MR

VP

UB

SCA

TB

MR

VP

UB

After inoculation:

SIM

Microbiology Laboratory
Group # 2
2G-PH
Members: Alodia O. Castillo
Alison A. Claveria
Jehhan N. Decampong
Angelica B. Dela Rosa

April 6, 2016
Paul D. Chiong
Regina DG. De Guzman
Aryssa Gem D. Dela Cruz

II.Pathogenicity Test For Staphylococci Mannitol Fermentation Test

Observations & Results:
MSA

Organism A

Organism B

Description of the Colony

Yellow colonies

Pink colonies

Interpretation of the Results

Mannitol fermenters

Non-mannitol fermenters

Before Inoculation:

MSA

After Inoculation:

MSA (A)
Name of Organism:
Staphylococcus aureus

MSA (B)
Name of Organism:
Staphylococcus epidermidis