Case of the Cyanide

Cocktail and the Missing

Pathogens
Audrey Lookner, Jake Kreiter, Katie Johnson, Estelle
Silk, Holly Bent

Victim Overview
Dr. Doodle Donley
Head of the Monarch University
Laboratory
The lab investigates bacterial and
viral pathogens
Expert in the microbiology field
Recently rewarded with the Scientist
of the Year award
Threw herself a party to

Incident Overview
Whole University was invited to Dr. Doodle Donleys party
Around 2 am guard was making rounds and noticed light was on in Dr.
Donleys Lab
The place was a mess from the party, but Dr. Donleys bruised and
battered body was also among the mess
University police was called at 2:05 am
When the police arrived they determined that there were virus
missing from her level 4 containment lab
The vials that were missing were: Smallpox, Mycobacterium tuberculosis, and
HIV

Crime Scene Overview

15 suspects including Dr.

Donleys twin
3 prime suspects were interviewed
Dr. Chip Tollhouse (suspect 3)
Dr. High (suspect 2)
Dr. Dangerkitty (suspect 1)

Blood Found at the Crime

scene

Lip prints and cyanide

found at the crime scene

Fingerprint at crime
scene

Crime Scene Walkthrough

https://drive.google.com/a/bvsd.org/file/d/0B7lp3AwlJGswVl9FRG1a
azVKWXFyOFJncl9mSF90eUMwWEpz/view?usp=sharing
Video of Crime Scene

Information on Pathogens
Smallpox
Variola major or smallpox
(virus)
Spread person to person
through aerosols of the
infected person or clothing
of the infected person
Symptoms: high fever,
malaise, headache, back
pain, rash
Treatment: postexposure
vaccination within 2-5 days
after infection, then after
the vaccination there is
supportive or antiviral drug
treatment

HIV
Human
immunodeficiency
virus
Methods of
contraction: sharing
needles, sharing of
bodily fluids, not
through casual contact
HIV has flu like
symptoms
Treatment: RT
inhibitors, Protease
inhibitors,
Combination drug
treatments
Earliest known case of
HIV was in 1959, it was

Mycobacteriu
m Tuberculosis

Tuberculosis or TB
TB is spread through the
air from person to
person, the bacterium is
put in the air when the
infected person sneezes,
coughs, etc.
Symptoms: pain in the
chest, chronic cough
(sometimes with blood),
chills, fever, loss of
appetite
Treatment: antibiotics
(Rifabutin, Ethionamide,
Isoniazid, etc.)
TB is fatal in infants

DNA Fingerprinting Method

DNA fingerprinting is a method of isolating and identifying variable elements
within a DNA sequence
Developed by Alec Jeffreys after he recognized that each human has a different
pattern of genes or DNA
Procedure of obtaining a DNA fingerprint:
Obtain a sample of cells
DNA is taken out of cell and purified
DNA are cut at specific points using restriction enzymes
The enzymes produce different lengths of DNA, they are then put in gel and the gel is then
put through an electric current towards a positive pole
Double stranded DNA is then put into single stranded and transferred to a nylon sheet

During the process the DNA went through a procedure called autoradiography

DNA Fingerprinting Analysis

After analysis of the DNA Fingerprints that were found at the crime
scene we can conclude that Dr. Chip Tollhouse was present. The
fingerprint found at the crime scene had the sizes of: band 1 - 19
mm, band 2 - 20.5 mm and band 3 - 32 mm. Dr. Chip Tollhouse had
the exact same size bands as the ones that were found at the crime
scene.
Fingerprints from Dr. Dangerkitty were also found but the band sizes
were not as closely matched as Dr. Chip Tollhouse

ELISA Method
ELISA (Enzyme-linked immunosorbent assay)
Plate based test that is designed to detect
substances such as peptides, antibodies,
proteins or hormones
In this test the antigen must be immobilized to
a solid surface and then complexed with an
antibody that links to the enzyme
Once both are attached to the bottom then the
well is rinsed out of any antibodies that
didnt bind
If the antibodies are present the indicator will

ELISA Test Simulation

ELISA Analysis
In our ELISA test both suspects who we tested, Subject #3 and
Subject #1, Tollhouse and Dangerkitty, both tested positive for
having the antibodies from the crime scene, traces of the
pathogens that were stolen. Placing both of the suspects at the
crime scene, stealing the pathogens.

Dr. High Interview

After conducting an interview this what we found:
Didnt attend the party
Instead he went to see a French Film, runs the projector
Doesnt have an alibi for the theater
Physicist not a biologist
Suspects Dangerkitty, knows a lot about toxins
Found Dr. Donley attractive at a time, but doesn't have a great
connection or relationship

Dr. Chip Tollhouse Interview

After conducting an interview this is what we found:
He attended the party because he was invited
Had some experience with toxins
Made up a lie about why his exact print was found at the crime scene
Was jealous of Dr. Donley because she could bake cookies while
transforming bacteria
Said that if she was out of the way he could win the Nobel Prize
Many people at the party also saw him lurking around the pathogens
that were stolen

Dr. Dangerkitty Interview

After conducting an interview this is what we found:
Went to the party
Experiences with toxins, used them on multiple occasions to kill
things, but claims to not have ever used them on a human
Relationship with Dr. Donley is not good, called her a brownnoser
Envies Dr. Donley because she says she is going to share, but doesn't
share the grant money

Who is Guilty???
Dr. Chip Tollhouse
(suspect #3)

Conclusion
After reviewing the case we have concluded the following
Dr. Dangerkitty was present at the crime scene and may have stolen the pathogens
He tested positive in the ELISA test for having the antibodies from the
pathogens
He was had very similar bands of his DNA fingerprint to the one at the crime
scene but it wasnt an exact match
Dr. Chip Tollhouse was also at the crime scene and we believe he is the one who hurt
Dr. Donley and stole the pathogens
He also tested positive in the ELISA test, proving that he had the antibodies
from the pathogens
He also had the exact same DNA fingerprint that was found at the crime scene,
same exact bands

Bibliography
Google. (n.d.). Retrieved April 18, 2016, from https://www.google.com/webhp?sourceid=chrome-instant
The Editors of Encyclopdia Britannica. (n.d.). DNA fingerprinting. Retrieved April 19, 2016, from
http://www.britannica.com/science/DNA-fingerprinting
https://www.youtube.com/watch?v=RRbuz3VQ100
Enzyme-linked immunosorbent assay (ELISA) overview. (n.d.). Retrieved April 19, 2016, from
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biologyresource-library/pierce-protein-methods/overview-elisa.html