Common Gram Negative

Bacteria
Aug. 2, 2016

1. Which of the following is true about

Neisseria?
A. Nonmotile and catalase and
oxidase positive
B. Inhibited by the presence of
vancomycin or lincomycin, colistin,
and nystatin
C. Anaerobic, gram-negative cocci
D. Catalase test is a key test for
identifying them

2. Gram-negative diplococci recovered

from an
MTM plate and giving a positive
oxidase test
can be presumptively identified as:
A. Neisseria gonorrhoeae
B. Neisseria meningitidis
C. Neisseria lactamica
D. All of the Above

3. Differentiation of N. gonorrhea and

N. meningitidis can be done by?
A. Glucose Fermentation
B. VP Reaction
C. Maltose Fermentation
D. Indole Test

4. The -galactosidase test aids in the

identification of which Neisseria
species?
A. N. lactamica
B. N. meningitidis
C. N. gonorrhoeae
D. N. flavescens

5. Cystine tryptic digest (CTA) media used for

identification of Neisseria spp. should
beinoculated and cultured in:
A. A CO2 incubator at 35C for 24 hours
B. A CO2 incubator at 42C for up to 72 hours
C. A nonCO2incubator at 35C for up to 72
hours
D. An anaerobic incubator at 35C for up to
72 hours

6. A useful test to differentiate

Moraxella catarrhalis from
saprophytic Neisseriae in respiratory
samples is:
A. Butyrate esterase
B. Gram stain
C. Growth on 5% sheep blood agar
D. PYR
E. Oxidase

7. Biochemically, the Enterobacteriaceaeare

gram-negative rods that:
A. Ferment glucose, reduce nitrate to nitrite, and
are oxidase negative
B. Ferment glucose, produce indophenol oxidase,
and form gas
C. Ferment lactose and reduce nitrite to
nitrogen gas
D. Ferment lactose and produce indophenol
oxidase

8. ETEC

9. EPEC

10. EHEC

11. What is the causative agent of

granuloma inguinale?

12. The swarming gram-negative bacillus

that can be described as oxidasenegative, nitrate-positive, indolenegative, and H2S-positive is mostly
likely which of the following?
A. Proteus aerogenes
B. Proteus vulgaris
C. Proteus mirabilis
D. Escherichia col

13. Which of the following is true

regarding the pathogenesis of
Shigella?
A. Essential pathologic process is
invasion of
the mucosal epithelial cells
B. Infective dose 105 organisms
C. Does not cause dysentery
D. Long incubation period (7-10 days)

14. The laboratory diagnosis of

Shigella are the following except:
A. Lactose Negative
B. Urease Negative
C. TSI: Alkaline (red slant)/acid (yellow
butt)
D. (-) gas , (-) H2S

15. The morphology and identification

of salmonella are the following
except
A. Gram Negative Bacilli
B. Motile with Peritrichous flagella
C. Ferments lactose or sucrose, H2S
producer
D. Sensitive to Brilliant Green, Sodium
deoxycholate, Sodium tetrathionate

16 -17. On Wilson Blair bismuth

sulphite media S. typhi produce
_____________ colonies due to
production of _____________

18. Which of the following is true

regarding the diagnosis of Enteric
fever?
A. Blood culture is positive in third
week
B. Stool culture is positive in first week
C. Titer against the O antigen of
greater than 1:320 is negative
D. Titer against the H antigen of
greater than 1:640 is considered

19. Which of the following is not true

about Vibrio cholerae?
A. comma-shaped, curved rod 24 m
long
B. actively motile by means of a polar
flagellum
C. grows well on TCBS which produces
yellow (sucrose fermented)
D. grows at a very low pH (3.5-4.5)

20-24. Which of the biochemical

characteristics are consistent with
Vibrio cholera? Choose
20. Catalase (Positive/Negative)
21. Oxidase (Positive/Negative)
22. Indole (Positive/Negative)
23. Nitrate reducers
(Positive/Negative)
24. Sucrose fermenter
(Positive/Negative)

25. Vibrio cholerae enterotoxin:

A. Increases the levels of intracellular
cyclic GMP
B. Heat-resistant enterotoxin
consisting of subunits A,B,C
C. Causes continued activation of
adenylate cyclase
D. Inhibits the enzyme
phosphodiesterase

26. Which of the following statements

regarding P. aeruginosa is false:
A. Gram (-), Aerobic, motile bacilli with
polar flagella
B. growth at 42C helps differentiate it
from other Pseudomonas species
C. Produces the fluorescent bluish
pigment pyocyanin
D. Many strains of P aeruginosa also
produce the fluorescent pigment

27. Hemorrhagic necrosis of skin

caused by P. aeruginosa is called?

28. Which of the following statements is/are true

regarding Yersinia pestis:
A. Produced pandemics of black death
with millions of fatalities
B. Associated with pneumonic plague transmitted by
aerosol
C. Rodents to Rodents transmission
D. Rodents to Human transmission by the bite of
fleas
E. Wild rodents are natural reservoirs of plague
F. All of the Above

29. What is the characteristic staining

of Y. pestis with special stains such as
Wright, Giemsa, Wayson, or
methylene blue

30-32

30
31
32

33. Which of the following statement is true

regarding B. pertussis?
1. Gram-negative coccobacilli
2. Capsule present
3. Aerobic; Oxidase (+); Catalase (+)
4. Nitrate (-); Urea(-); Citrate(-)
A. 1,2,3, and 4 are correct
B. 1,2, and 3 are correct
C. 1 and 2 are correct
D. Only 1 is correct

What is the other name for BordetGengou medium (34. Identify) which
contains (35. Identify the antibiotic)
However, a charcoal-containing
medium supplemented with horse
blood, cephalexin, and amphotericin
B (36. Identify the Other name) is
preferable because of the longer shelf
life

37. Gram stain of Campylobacter jejuni

showing
(Identify: xxxxx or xxxx xxxx)
shaped gram-negative bacilli

38. Dark-field or phase contrast

microscopy may show the typical
(Identify________ motility of the
organisms.

39. A child was diagnosed to be

suffering from diarrhea due to
Campylobacter jejuni. Which of the
following will be the correct
environmental conditions of incubation
of culture plates of the stool ?
A. Temperature of 42C, 4% O2, 15% CO2
B. Temperature of 42C, 5% O2, 10% CO2
C. Temperature of 37C, 4% O2, 15% CO2
D. Temperature of 37C 5% O2, 10% CO2

40. Which of the following correctly

describe H. pylori:
A. Gram negative cocci curved
B. Gram negative cocci straight
C. Gram negative motile bacilli spiral
rod
D. Gram negative bacilli straight
E. Gram negative motile bacilli

Routine Media
41. What is considered a positive result
of MacConkey Agar?

42-43 Give at least 2 Lactose

fermenters enterobacteriaceae
44-46 Giver at least 3 Non Lactose
fermenters enterobacteriaceae

47. What is a positive result of indole

test?
48. What reagent is added into
tryptophane broth after incubation of
37 C for 24 hours

Methyl Red-Voges Proskauer

Test
49. Which organism will yield MR
positive test result ? Choose E.
aerogenes / E.coli
50. Which organism will yield VP
positive test result ? Choose E.
aerogenes / E.coli

51. Citrate test positive:

E.coli/K.pneumoniae

Bonus