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1. Labium oris
Musculus Obicularis Oris is present (skeletal muscle)
On the external surface is the skin with its adnexa
On the inner surface is the mucosa (stratified squamous epithelium +
lamina propria) and submucosa with mixed glands Labial glands).
2. Apex lingua
Underneath the papillae, there are mucous and serous glands, pockets of
adipose tissue, and a layer of skeletal muscle and connective tissue.
The skeletal muscle is arranged in three different planes, which allows the
tongue to perform a number of complex movements.
3. Papilla Circumvallata
Surrounded by a deep furrow, where Ebners Glands open.
Epithelia covering papilla (stratified squamous non-keratinizing).
Taste buds are located on the lateral sides, in the epithelium.
4. Tonsilla lingualis
A lymphatic structure that contains abundant germinal centers where
immune cells undergo differentiation.
Surface is covered with stratified squamous epithelium.
5. Soft palate:
On the nasal side it is possible to see:
pseudostratified epithelium
- lamina propria
- submucosa with mixed glands Nasal Glands
5. Soft palate:
On the oral side it is possible to see:
lining mucosa of the mouth cavity
- submucosa with mucous glands Palatine Glands
5. Hard palate:
Covered by masticatory mucosa (stratified squamous epithelium)
Note bone at the top, mucosa at the bottom and a large amount of salivary
tissue between.
A short distance from the lamina propria there is a large duct.
6. Tonsilla palatina
The tonsils share some histological features with lymph nodes:
1. Cells in the tonsils are supported by a fine network of reticular fibres
2. High-endothelial (postcapillary ~) venules function in the "homing" of
circulating lymphocytes - this is actually a shared feature of all lymphoid
tissues and organs.
The palatine tonsils are surrounded by a thick hemicapsule of connective
tissue, which delimits them from the pharyngeal muscle and facilitates their
removal in tonsillitis.
7. Tooth
Pulp jelly-like connective tissue with blood capillaries and fine nerve
fibers. Odontoblasts are located on the external surface.
Dentin shows fine striation, caused by dentin tubules (contain the
cytoplasmic processes of odontoblasts); dentin is stained in red or redviolet color, except thin layer near the odontoblasts predentin (is not
calcified) and peripheral layer below cementum and enamel (is
irregularly calcified), dentin is pale in this layers.
Enamel is not present in decalcified tooth (it dissolves during
decalcification).
Cementum covers the root(s) of the tooth; the thickest layer is on the
root apex(es); it is not possible to distinguish primary (acellular) and
secondary (cellular; with cementocytes) cementum in the light
microscope.
Periodontium bundles of collagenous fibers, which hold the dental root
in bone alveolus (rarely present in slides).
8. Parotid Gland
A, Serous acini; B, Striated ducts; C, Ecretory duct
Parotid gland only serous (alveolar = acinar) gland
Only serous
intercalated striated interlobular main
The gland is internally divided into lobules. Blood vessels and nerves enter
the glands at the hilum and gradually branch out into the lobules.
Consists of paired glands surrounded with a connective tissue capsule; septa
running from the capsule separate the glandular parenchyma into lobes and
lobules. Blood vessels, nerves and interlobular ducts are found in the
connective tissue of septa. Parenchyma is composed of intralobular ducts
and secretory portions of glands.
They consists of connective tissue = capsule + septa and
parenchyma of lobules = ducts + secretory portions
9.Submandibular Gland
A, Mucous acini; B, Serous acini
mixed (80 % serous)
serous acini
mucouse tubuli
intercalated striated interlobular main
+ lunuly of Ginuzzi
11. Oesophagus
The esophagus consists of three layers: mucosa, submucosa, and muscularis.
The mucosa consists of epithelial lining containing nonkeratinizing, stratified
squamous epithelium with a layer of basal and parabasal cells. This layer is
naturally required to proliferate more often than other layers of the
oesophagus in accordance to necessary cell loss.
The sublayer, lamina propria, contains vessels, connective tissue, lymphatics,
inflammatory cells and esophageal cardiac glands which are mucus secreting
glands. The submucosa contains dense connective tissue with both lymph
and blood vessels. Further esophageal mucus secreting glands are contained
in the submucosa. The outer layer, the muscularis, consists of two muscle
layers. The inner muscle layer fibers are arranged circumferentially and the
outer layer, longitudinally.
12. Cardia
On histological examination, the junction can be identified by the following
transition.
nonkeratinized stratified squamous epithelium in the esophagus
simple columnar epithelium in the stomach
The cardiac glands can be seen in this region.
They can be distinguished from other stomach glands (fundic glands and
pyloric glands) because the glands are shallow and simple tubular.
13.Fundus ventriculi
(vertical section to the surface of mucosa, HE)
A.
14. Pylorus
deeper gastric pits, reticular conn. tissue, pyloric glands
15. Duodenum
(longitudinal section through the wall, HE)
A. Tunica mucosa typical organization: villi intestinales; crypts of
Lieberkhn
1. lamina epithelialis mucosae simple columnar epithelium
composed of absorptive cells and secretory cells (see
descrition of the intestinum tenue)
2. lamina propria mucosae - reticular connective tissue with
lymphatic nodules (formes underlying tissue of intestinal
villi and surrounds the crypts of Lieberkhn),
3. lamina muscularis mucosae smooth muscle tissue.
B. Tela submucosa loose connective tissue whith mucous glands
Brunners glands (an important signe of this part of small intestine!).
C. Tunica muscularis (externa) - 2 layers of smooth muscle cells: inner
circular and outer longitudinal.
D. Tunica serosa see description of the stomach.
19. Hepar
(Hematoxylin-eosin or Azan)
A.
Functional unit:
portal lobule (lobule of portal vein) parts of parenchyma of 3
morphological units surrounding common portal area (triangular region with
portal area in the center join central veins of 3 nighbour liver lobules)
smaller unit is liver acinus parencyma of 2 liver lobules arround common
circumlobular side.
2.
B.
C.
D.
21. Pancreas
(Hematoxylin - eosin)
A.
22. Epiglottis
Epiglottis prevents food from entering the trachea.
Structure:
plate of elastic cartilage
lingual side: stratiffied squamous epithelium + lamina
propria,
- laryngeal side: pseudostratified columnar epithelium with cilia + mixed
glands in lamina propria,
23. Larynx
This area of the larynx is typified by two items: (1) hyaline cartilage (lower
left) and (2) sero-mucous glands (upper right). Epithelium is usually
pseudostratified columnar ciliated.
the wall structure:
- mucosa epithelium of respiratory passages + lamina propria with elastic
fibers and mixed glands
- fibrocartilagenous layer with
large hyaline and small elastic cartilages
- external muscle coat = skeletal muscle tissue
The organ of fonation
- laryngeal ventricle (vestibule)
- plica vocalis (with stratified squamous epithelium, elastic ligament
ligamentum vocale and skeletal muscle musculus vocalis)
- plica ventricularis (the same structure as in the larynx wall
24. Trachea
Hollow tubular organ structure of its wall:
- mucosa epithelium of respiratory passages + lamina propria with elastic
fibers and mixed glands
- fibrocartilagenous layer with ringshaped, dorsaly opened hyaline cartilages (16 20), dorsal part of the wall is
called paries membranaceus cartilage is substituted by tracheal muscle
(smooth)
- adventitia (connective
tissue with nerves and blood vessels)
The lungs are large, lobed, paired organs in the chest (also known as the
thoracic cavity). Thin sheets of epithelium (pleura) separate the inside of the
chest cavity from the outer surface of the lungs. The bottom of the thoracic
cavity is formed by the diaphragm
26. Ren
(perpendicular section to the surface of kidney, HE or HES
and Weigert-van Gieson)
A.
^cortex
^medulla
poles.
2.
B.
C.
28. Ureter
(cross section, HE)
A.
29. Testis
(Hematoxyline-eosin /HE/ or - safron /HES/)
A.
2.
30. Epididymis
(Hematoxyline-eosin /HE/ or - safron /HES/)
A.
33. Prostata
(Hematoxyline-eosin or Azan)
A.
34. Penis
(Cross section, HE)
A.
35. Ovarium
(perpendicular section to the surface of ovary, HE or HES)
First note that the ovary has a cortex-medullary
organization and is surrounded by a thick connective tissue
capsule, the tunica albuginea. The surface of the tunica
albuginea is covered with a cuboidal, germinal epithelium.
This slide illustrates all the phases of follicular
development.
A.
following slide,
corpora albicantia (pl.) they have different
shape and size and are made up of hyalinized c.t.
C. Medulla of the ovary (zona vasculosa) dense c.t.
with spiral blood vessels, lymph vessels and nerves.
5.
Tunica mucosa:
1. lamina epithelialis mucosae simple columnar
epithelium, composed of ciliated cells (CC) and
secretory cells (SC),
2. lamina propria mucosae loose c.t.
B. Tunica muscularis smooth muscle cells (circular and
longitudinal layer).
C. Tunica serosa simple squamous epithelium
(mesothelium) + thin layer of loose c.t.; subserous c.t.
with ovarian and uterine vessels is between serosa and
tunica muscularis.
AMPULLA
Epithelium - primary, secondary and terciary folds,
which fill the lumen,
abundant ciliated cells [CC > SC]
External Muscle coat / layer - thin irregular
^Pars Ampullaris
37. Isthmus
Epithelium - sporadic simple longitudinal folds,
-abundant secretory cells [SC > CC]
^Pars Isthmica
C.
40. Vagina
(Hematoxylin-eosin or Bests carmine)
A.
Connective tissue:
1. thin c.t. capsule (pia mater) and fine septa s
with blood vessels,
2. loose interstitial c.t. with blood sinusoids.
B. Parenchyma of epiphysis:
1. cords and groups of 2 types of cells
pinealocytes and neuroglial cells,
2. unmyelinized nerve fibers,
3. acervulus cerebri intensly stained calcified
corpuscles (brain sand) of irregular shape.
Connective tissue:
1. capsule from dense collagenous c.t. with blood
and lymph vesels and nerves,
2. c.t. septa are distinct and separate gland into
the lobules,
3. interstitial c.t. loose c.t. with net of capillaris,
around follicles.
B. Lobules consist of follicles lined with epithelium
and containing colloid:
1. epithelium in follicle simple, its high depends
on functional stage and size of follicle; it is
composed of follicular and parafollicular cells
(parafollicular cells can be idetified only with
using of special staining methods),
2. colloid fills cavity of each follicle, it has
homogenous appearance and is usually stained
with acid dyes.
Connective tissue:
1. thin c.t. capsule and septa with blood vessels
and nerves,
2. net of reticular fibers (separate glandular cells
and blood capillaries).
B. Parenchyma cords and groups of glandular cells:
1. chief cells predominate, with light basophilic
cytoplasm,
2. oxyphil cells with intensly eosinophilic
cytoplasm (they are lined in older slides, its result
of discoloration) they occure after 10th year of
age.
Connective tissue:
1. dense c.t. capsule and thin radial septa with
blood vessels,
2. net of reticular fibers around cords of glandular
cells and blood sinusoids.
B. Parenchyma - 9/10 form cortex, the rest represents
medulla:
1. cortex:
zona glomerulosa bellow c.t. capsule, cell
cords are coiled like glomerule, cell cytoplasm
is basophilic,
zona fasciculata middle, the most
voluminous layer with paralelly oriented cell
cords (perpendicularly to the capsule); cells
are large, vakuolized (spongiocytes),
zona reticularis cell cords anastomose and
form network (reticulum); cells are small,
eosinophilic and contain granules of
lipofuscin,
2. medulla:
cords and groups of chromaffine cells with
finely granular, basophilic cytoplasm,
nerve fibers and solitary ganglionic cells.
47. Thymus
The thymus is a highly lobulated organ
invested by a loose collagenous capsule, C,
from which short interlobular septa, S, containing
blood vessels radiate into the
substance of the organ.
The thymic tissue is divided into two distinct
zones, a deeply basophilic outer cortex,
Cx, and an inner eosinophilic medulla,
M; distinction between the two zones is most
marked in early
childhood as is represented in this specimen.
-
49. Aorta
Large arteries = Elastic arteries (examples: aorta
and and pulmonary arteries, the immediate
branches of these arteries are also considered to
be elastic arteries brachiocephalic, common
carotids, etc.). have sheets of elastic tissue in their
walls and are the largest diameter arteries. They
are subjected to high systolic pressures. These
large vessels are also adapted to smooth out the
surges in blood flow since blood only flows
through them during systole. The elastic tissue in
their tunic media provides the resilience to smooth
out this pressure wave.
The tunica intima is relatively thick and consists of
an endothelial layer with its basal lamina, a
subendothelial layer of connective tissue and an
internal elastic membrane. The internal elastic
membrane is not as conspicuous because it is one
of the many elastic membranes in the wall of the
arteries. It is usually only identified because it is
the innermost of the elastic layers of the arterial
wall.
The tunica media is the thickest of the three
layers. The numerous, thick, fenestrated elastic
membranes are the predominant substance of the
media. They are interconnected by fine elastic
fibers and also by smooth muscle cells, which
spirals at a slight angle to the transverse axis of the
vessel. The smooth muscle cells is surrounded by
reticular fibers and a few collagenous fibers.
In elastic arteries, the tunica adventitia is
relatively thin half the thickness of the media
and contains collagen fibers, elastic fibers and
connective tissue cells (fibroblasts and
macrophages). The tunica adventitia contains
blood vessels (vasa vasorum) and nerves (nervi
vasorum) that supply the blood vessel wall.
capsule
Red pulp
White pulp
parenchym = pulp:
- white pulp: is a typical lymphatic tissue
with lymphocytes in reticular c.t.,
lymphocytes are located around central
arteris forming so called periarterial
lymphatic sheath (PALS), this sheaths is
sometimes extended into lymphatic
nodules (follicle) = bodies of Malpighi.
PALS contains T-lymphocytes, in nodules
and on the periphery of PALS Blymphocytes and macrophages and
dendritic cells are present. Periphery of
PALS and nodules is called marginal zone
and contains also many blood sinuses.
- red pulp: is composed of reticular c.t.
with different cell types around sinuses.
Free cells are macrophages, lymphocytes,
granulocytes and mainly erythrocytes.
Reticular c.t. with cells form cords of
Billroth and these cords surrounds venous
sinuses.
3. Blood circulation:
A. lienalis entres the splenic hilus its branches
pass through the trabecules as aa. trabeculares
when they leave the trabecules, they are called aa.
centrales and are followed by lymphatic tissue
(PALS + boides of Malpighi) these arteries are
branched at the end into arteriolae penicilatae:
eache arteriole has 3 parts:
1. arteriole of pulp (medullary arteriole),
2. encapsulated arteriole (surrounded by
57. Nail
61. Cerebellum
(Hematoxyline-eosin, Nissl or impregnation)
A.
C.
Connective tissue:
1. dense c.t. capsule,
2. septa with blood vessels
3. loose c.t. around ganglionic cells.
B. Ganglionic cells are in groups, usually bellow c.t. capsule:
1. ganglionic cells round or oval pseudounipolar neurons
with light vesicular nucleus and granules of lipofuscin in
cytoplazm,
2. satelite cells (amficytes) spindle-shaped cells with dark
nuclei, cells form continouous coat around each ganlionic
cell
C. Bundles of nerve fibers with myelin sheath run throughout
middle zone of ganglion.
B.
C.
D.
E.
Cornea:
1. anterior epithelium stratified squamous
epithelium,
2. Bowmans membrane 6-9 m thick membrane
of homogenous appearance,
3. substantia propria is composed of collagenous
lamelae oriented in paralel way with the surface,
amorpheous substance and flattened fibrocytes,
4. Descemets membrane basal lamina of
posterior epithelium of corneae,
5. posterior epithelium (endothelium) simple
squamous epithelium of mesenchymal origin.
Sclerocorneal junction - contains sinus venosus
(canal of Schlemm).
Corpus ciliare is triangular in section, processus
ciliares are prominent on its free surface:
1. connective tissue stroma highly vascularized,
around musculus ciliaris, which is composed of
bundles of smooth muscle cells oriented in
meridional, radial and circular direction,
2. pars ciliaris retinae 2 layers of epithelial cells:
outer layer with pigment cells continuation of
stratum pigmenti retinae,
inner layer with columnar cells without
pigment is atteched to the membrana
limitans interna.
Iiris - thin c.t. plate with pupilla in the center,
situated between anterior and posterior chamber of
the eye. Laeyers of iris:
1. anterior epithelium endothelium (is dificult to
identify it in the slide),
2. anterior limiting (stromal) sheath condensed
c.t. and pigment cells,
3. stroma iridis yelly-like c.t. with melanocytes
and blood vessels, smooth muscle is near pupilla
(musculus sphincter pupillae),
4. posterior limiting sheath is very thin, composed
of myoepithelial cells, forming musculus dilatator
pupillae,
5. posterior epithelium = pars iridica retinae layer
of columnar highly pigmented cells continuation
of pars ciliaris retinae
Lens (lens cristallina) (can be missing in some
slides):
1. capsula lentis homogenous membrane on lens
surface,
2. anterior epithelium cuboid epithelium between
capsule ventral side of lens,
3. lens fibers the most important part of lens.
67. Palpebra
(cross section, HE or HES)
A.
68. Auricle
Ec = elastic cartilage
* = typical thick skin with hair follicles and sebaceous
glands
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69.
http://www.google.com/imgres?q=sclera+choroid+retina+slide&um=1&hl=en&client=safari&sa=N&rl
s=en&biw=1280&bih=630&tbm=isch&tbnid=Pqy7ziXeZhPHrM:&imgrefurl=http://histologyworld.com/photoalbum//displayimage.php%3Fpid%3D1104&docid=9rzX3UZT4DKGAM&w=400&h=299&ei=F
wSDTuebKs_WsgaQxO21Dg&zoom=1&iact=hc&vpx=668&vpy=149&dur=1069&hovh=194&hovw=260&tx=155
&ty=119&page=1&tbnh=133&tbnw=198&start=0&ndsp=19&ved=1t:429,r:3,s:0
70.
http://www.google.com/imgres?q=palpebra+slide&um=1&hl=en&client=safari&sa=N&rls=en&biw=1
280&bih=630&tbm=isch&tbnid=a66hHYPKzaHnM:&imgrefurl=http://www.iupui.edu/~anatd502/Labs.f04/eye%2520lab/Eye%2520Lab.html&docid=CFNV
73tb8jPV1M&w=212&h=576&ei=AwWDTvDCHs_ysgaPk9CNDg&zoom=1&iact=hc&vpx=611&vpy=154&dur=34
4&hovh=294&hovw=108&tx=70&ty=174&page=3&tbnh=132&tbnw=49&start=36&ndsp=18&ved=1t:429,r:8,s:
36
http://www.google.com/imgres?q=umbilical+cord+slide&um=1&hl=en&client=safari&rls=en&biw=1280&bih=
652&tbm=isch&tbnid=BHtgt5nhG6DnjM:&imgrefurl=http://www.kumc.edu/instruction/medicine/anatomy/hi
stoweb/ct/ct14.htm&docid=bTclbQ1WqHfseM&w=406&h=268&ei=V_CTtmxM7H24QSAzfVs&zoom=1&iact=hc&vpx=367&vpy=158&dur=444&hovh=182&hovw=276&tx=174&ty=98
&page=1&tbnh=140&tbnw=182&start=0&ndsp=16&ved=1t:429,r:1,s:0
71.
http://www.histologyworld.com/photoalbum/displayimage.php?album=86&pid=1445#top_display_media
72.
http://www.histologyworld.com/photoalbum/displayimage.php?album=86&pid=1444#top_display_media