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  • Table 1: Primer Sets Designed For This Assays Assay Target Primer Name Sequence (5'-3') References

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    josekin
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    The document describes primer sets designed to target specific genes in Aeromonas bacteria. Table 1 lists the primer names, sequences, and references for assays targeting the 16S rRNA gene and genes for aerolysin, haemolysin, and lipase. Table 2 shows the biochemical characteristics of three Aeromonas isolates, including their reactions to Gram staining, motility, catalase production, and utilization of various carbohydrates. Table 3 validates real-time PCR assays on the isolates, reporting Ct values for the aerolysin, haemolysin, and lipase genes. Table 4 examines the susceptibility of A. veronii to 20 antibiotics, identifying levels of resistance, susceptibility, and intermediate resistance.

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    The document describes primer sets designed to target specific genes in Aeromonas bacteria. Table 1 lists the primer names, sequences, and references for assays targeting the 16S rRNA gene and genes for aerolysin, haemolysin, and lipase. Table 2 shows the biochemical characteristics of three Aeromonas isolates, including their reactions to Gram staining, motility, catalase production, and utilization of various carbohydrates. Table 3 validates real-time PCR assays on the isolates, reporting Ct values for the aerolysin, haemolysin, and lipase genes. Table 4 examines the susceptibility of A. veronii to 20 antibiotics, identifying levels of resistance, susceptibility, and intermediate resistance.

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    24 vizualizări4 pagini

    Table 1: Primer Sets Designed For This Assays Assay Target Primer Name Sequence (5'-3') References

    Încărcat de

    josekin
    The document describes primer sets designed to target specific genes in Aeromonas bacteria. Table 1 lists the primer names, sequences, and references for assays targeting the 16S rRNA gene and genes for aerolysin, haemolysin, and lipase. Table 2 shows the biochemical characteristics of three Aeromonas isolates, including their reactions to Gram staining, motility, catalase production, and utilization of various carbohydrates. Table 3 validates real-time PCR assays on the isolates, reporting Ct values for the aerolysin, haemolysin, and lipase genes. Table 4 examines the susceptibility of A. veronii to 20 antibiotics, identifying levels of resistance, susceptibility, and intermediate resistance.

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    din 4

    Table 1 Primer sets designed for this assays

    Assay target
    16SrRNA genes

    Primer name

    Aerolysin gene

    AVAF
    AVAR
    Hem F
    Hem R
    Lip F
    LIP R

    Haemolysin
    gene
    Lipase gene

    sequence (53)
    F:5-AGAGTT TGATCCTGGCTCAG-3
    R:5-TACGGCTACCTTGTTACGACTT-3
    5-GAGAAGGTGACCACCAAGAACA-3
    5- GGTAGGAGATTGGCTTGTAGAG -3
    5-CACCGGTTTCCGGAGAAATA-3
    5-CCCAGGCGTATCCCAAATAA-3
    5-CGACCTATACCTGTAGGCTTCT-3
    5-TGCGGGAGGAATAGGATTGA-3

    References
    Xiao et al. 2009
    This study
    This study
    This study

    Table 2.Biochemical characteristics of the isolates


    Test items
    Gram staining

    WHMA3 WHMA2
    -

    WHMA1
    -

    Motility

    Oxidase

    Catalase

    Glucose

    Sucrose

    Lactose

    Arabinose

    Mannitol

    Aesculin

    Methyl red

    V-P reaction

    Citrate Utilization

    Esculine Hydrolysis

    Indole Production

    H2S Production

    Satrch hydrolysis

    Aeromonas media

    Table 3 Validation of real-time PCR assays using Aeromonas veronii


    Aerolysin

    Hemolysin

    Lipase

    35.21
    34.93
    34.25

    32.76
    30.93
    32.10

    32.92
    31.73
    31.26

    Strain
    WHMA1
    WHMA2
    WHMA3

    Table 4 Susceptibility of A.veronii to 20 various antibiotics


    Antibiotics

    Diameters of Inhibition

    Susceptibility

    Zone (mm)
    19

    Florfenicol

    36

    Levofloxacin

    27

    Tetracylin

    13

    Neomycin

    17

    Novobiocin

    31

    Doxycylin

    19

    Gentamicin

    Norfloxacin

    27

    Aztreonam

    39

    Fortum

    26

    Kanamycin

    16

    Ceftriaxone sodium

    45

    Ofloxacin

    26

    Piperacillin

    NR

    Clindamycin

    NR

    Rifampicin

    22

    Imipenem

    NR

    Erythromycin

    NR

    Amoxilin

    Chloramphenicol
    32
    S
    R, resistance; S, susceptible; I, intermediate ; NR, no resistance

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