pCHAC-mWasabiGFP-2A-MCS

Cat#: ABP-PVL-GFP2A01
R etroviruses are one of the most efficient tools for delivering genes into dividing mammalian cells. The pCHAC-mWasabi-2A-MCS
Retroviral Expression Vector is a Moloney Murine Leukemia Virus (MMLV) based vector containing retroviral LTRs and packaging signal.
In addition, the vector also includes the bright monomeric GFP, mWasabi. A multiple cloning site (MCS on the map below) is present, which
can be used to insert desired cDNAs separated by 2A peptide sequence. This setup allows bicistronic expression from one transcript with the
flexibility of putting other proteins as the trailing translation unit.
The 2A-based bicistronic expression has been used for several years, but recently gained much more popularity due to its successful use in
iPSC generation which required 2 to 4 factors working in concert. Even expression of all factors can be achieved when 2A peptides are used
for multiple protein production, due to near 100% efficiency of the 2A “cleavage” at each site, and no interference between multiple 2A sites.

Amp R
5' LTR

pUC ori pCHAC-mWasabi-2A-MCS

pCHAC-MCS-IRES-mWasabi
6356 bp

mWasabi

MCS
3' LTR
2A Peptide
IRES
MCS
mWasabi GFP

Comments for pCHAC-MCS-IRES-mWasabi

F or Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transfer
this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted
421-1012 5 ’ LTR
use without the express written authorization of Allele Biotech is strictly prohibited
2307-2350 MCS
2351-2929 IRES
Allele Biotech-Introducing Cost Effectiveness to Research
2942-3652 mWasabi GFP
3765-4356 3 ’ LTR
4631-5270 pUC Origin