Alleleustrious pmTFP1-Alpha-Actinin

Source Catalog Number: Introduction All ustrious pmTFP1-alpha-actinin is a mammalian expression vector that encodes a fusion of human alpha-actinin (ACTN1) N-terminal to mTFP1. It can be a great tool for visualizing cytoskeleton. The properties of All ustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in the mitotic spindle of mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In muscle cells, alpha-actinin is necessary for the attachment of actin filaments to the z-line membrane. The functional protein of alpha-actinin is an anti-parallel dimer, which cross-links the thin filaments in adjacent sarcomeres, and therefore coordinates contractions between sarcomeres in the horizontal axis. In skeletal, cardiac, and smooth muscle cells, isoforms of actinin are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
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ABP-FP-TALAC

Engineered variant of Clavularia sp. fluorescent protein cFP484. Recommended Use To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended. Features More than 3-fold brighter than ECFP More than twice as photostable as ECFP Single emission peak Single-exponential fluorescence lifetime ( = 3.2 ns) Can be co-imaged with red and yellow FPs Low sensitivity to acidic pH (fluorescence pKa=4.3) Mammalian expression vector ready to transfect your favorite cells True monomer that will not aggregate or cause non-specific interactions Reconstitution 5 g provided in lyophilized powder form. Reconstitute with 5 L of nuclease-free water for a final concentration of 1 g/L. Storage Store at -20C or at -80C for long-term preservation.

Human CMV Immediate-Early Promoter (CMV Promoter)........1-589 Human alpha-actinin.................637-3312 Linker..3313-3369 mTFP1.3370-4080 SV40 PolyA Signal.....................4232-4282 bla Promoter...............................4821-4925 Ampicillin Resistance Gene......4910-5770 pUC Origin..................................5919-6561 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C BLA SV40 Poly A mTFP1 Linker AMPR pUC Ori PCMV

pmTFP1Alpha-Actinin 6643bp

AlphaActinin

References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09. Reinhard M, Zumbrunn J, Jaquemar D, et al. (1999). "An alpha-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment.". J. Biol. Chem. 274 (19): 134108. Knudsen KA, Soler AP, Johnson KR, Wheelock MJ (1995). "Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.". J. Cell Biol. 130 (1): 6777.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Alpha-Actinin
Sequence
1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 2341 2401 2461 2521 2581 2641 2701 2761 2821 2881 2941 3001 3061 3121 3181 3241 3301 3361 3421 3481 3541 3601 3661 3721 3781 3841 3901 3961 4021 4081 4141 4201 4261 4321 TAGTTATTAA CGTTACATAA GACGTCAATA ATGGGTGGAG AAGTACGCCC CATGACCTTA CATGGTGATG ATTTCCAAGT GGACTTTCCA ACGGTGGGAG CCGGACTCAG ACCAACGATT TGGGAGAAGC GGGACACAGA CTGGAGGTCA AAGATCTCCA TCCATCGGAG ACCATCATCC GGGCTGCTCC TTCCACATAA GAGCTGATTG TTTGACGTGG GGAACTGCCC TTCTCTGGAG AACCAGGAGA TGGATCCGCC ATGCAACAGA CAGGAGAAGT AACCGGCCTG GGCTGCCTGG CTGGAGCGAC TGGACTGACG GAGATCAAGG GACCGTGTGG TCACCCAGTG CTAACTCAGA CAGCTGTACT ATGGAGGACC ACAGCCCATG CTGGGCATCC ACCAACCCCT CAGCTGGTGC GAGAGGCTAC AAGATGGAGG AGCCACCTGC GAGGGCGACC ACCATGGAGC AATGAGGTAG AATGAGTTCC GAGGAGTTCA GAAGCAGAAT TTCCAGGCCT GTCATGGCTT CGCCGCGAGC GGCCCCGACT GAGAGTGACC GTCGCCACCA AAGATCAAGC GGCGAGGGCA CCCCTGCCCT ACCAAGTACC TGGGAGCGCA ATGGAGGAGG GGCCCCGTGA CGCGACGGCG CACCGCGTTG CACTTTGTGG GTTTACGAGA GCGGCCGCGA AAAAAACCTC TAACTTGTTT AAATAAAGCA TTAACGCGTC TAGTAATCAA CTTACGGTAA ATGACGTATG TATTTACGGT CCTATTGACG TGGGACTTTC CGGTTTTGGC CTCCACCCCA AAATGTCGTA GTCTATATAA ATCTCGAGCT ACATGCAGCC AGCAGAGAAA TCGAGAACAT TCTCAGGTGA ACGTCAACAA CCGAAGAAAT TGCGCTTTGC TGTGGTGTCA GCTGGAAGGA ACTACGGGAA CAGAGAAGTA GACCGGATGA CCCAGAAGGC ACGAGCAGCT GCACAATCCC AGCTGGAGGA GCCAGCTGGA CCTTCATGCC AGCAGGTGGA TGGACCACCT GCAAAGAGGC CCCTGCTCAA AGCAGATTGC TCAACGCCCG AGCGAAGGGA TGGAGTATGC TGCAGGACAC AGCAGTTCAA ACAATGAGGT ACACAACCAT CTCGGAGGGA GCAAGCAGTT AGATCGGGAG GGCAGTATGA ACCAGCTCAT ACATCCGTGT AGAACCAGAT GGGCCTCCTT AAGCCTGCCT TTGCCCGCAT TCATTGACTT CCTTCAAGAT TGCCACCCGA CCGTGCCAGG TCAAGCTTCG TGGTGAGCAA TGAAGATGGA AGCCCTACGA TCTCCTACGA CCGACGACAT CCATGACCTT ACTCCTTCAT TGCAGAAGAA TGCTGAAGGG ACTTCAAGAC ACCACCGCAT GCGCCGTGGC CTCTAGATCA CCACACCTCC ATTGCAGCTT TTTTTTTCAC CTGTAGCGGC TTACGGGGTC ATGGCCCGCC TTCCCATAGT AAACTGCCCA TCAATGACGG CTACTTGGCA AGTACATCAA TTGACGTCAA ACAACTCCGC GCAGAGCTGG CAAGCTTCGC AGAAGAGGAC GACATTCACG CGAAGAGGAC ACGCTTGGCC GGCCCTGGAT CGTGGATGGG CATCCAGGAC GAGAAAGACA TGGCCTCGGC GCTGCGGAAG CCTGGACATC GAAAGCCATC GGAGACAGCA TATGGAAGAC GTGGCTGGAG CTTCCGGGAC GATCAACTTC CTCTGAGGGC GAAGGGCTAT GGCAGAGAAG CATGCTGCGA GAAGCATGAG CGCCATCGCA TTGCCAAAAG AGCTCTGGAG CAAGCGGGCT CTTCATTGTG GGCCACCCTC GTCCAAGATT CACGCCTCAG CCAAGCTCTG TGGAGCCCAG GATCTCCATT GAAGAGCATC CCAGGAGGCG GGGCTGGGAG CCTGACCCGG CAACCACTTT CATCAGCTTG CATGAGCATT CATGTCCCGC CCTGGCTGGG CCAGGCTGAG TGCTCTGGAC AATTCTGCAG GGGCGAGGAG GGGCAACGTG CGGCACCAAC CATTCTGACC CCCCAACTAC CGAGGACAAG CTACGAGATA GACCACCGGC CGACGTCAAG CATCTACAGG CGAGATCCTG CCGCAACTCC TAATCAGCCA CCCTGAACCT ATAATGGTTA TGCATTCTAG GCATTAAGCG ATTAGTTCAT TGGCTGACCG AACGCCAATA CTTGGCAGTA TAAATGGCCC GTACATCTAC TGGGCGTGGA TGGGAGTTTG CCCATTGACG TTTAGTGAAC ACCATCATGG TGGGACCGGG GCATGGTGTA TTCCGGGATG AAGCCAGAGC TTCATAGCCA AATGTGAAGA ATCTCCGTGG GCCCCTTACA TTCTGTGCTT GATGATCCAC CCCAAGATGC ATGACTTACG GCCAATCGCA TACGAGAAGC AACCGGGTGC TACCGGCGCC AACACGCTGC AGGATGGTCT GAGGAGTGGT TTCCGGCAGA CAGAAGGACT GCCTTCGAGA CAGGAGCTCA ATCTGTGACC CGGACCGAGA GCACCCTTCA CACACCATTG CCTGATGCCG GTCCAGACCT GAGATCAATG ACGGAGGAGC GCCAATGTCA GAGATGCATG GTCAACTACA CTCATCTTCG CAGCTGCTCA GATGCCAAGG GACCGGGATC GGTTATGATA GTGGACCCCA GAGACAGCCG GACAAGAACT TACTGCATCG TACATGTCCT TCGACGGTAC ACCACAATGG AATGGCCACG ACCATCAACC ACCGCGTTCG TTCAAGCAGT GGCATCGTGA CACCTCAAGG TGGGACGCCT CACAAGCTGC GCCAAGAAGG AACCACGACA ACCGACGGCA TACCACATTT GAAACATAAA CAAATAAAGC TTGTGGTTTG CGGCGGGTGT AGCCCATATA CCCAACGACC GGGACTTTCC CATCAAGTGT GCCTGGCATT GTATTAGTCA TAGCGGTTTG TTTTGGCACC CAAATGGGCG CGTCAGATCC ACCATTATGA ACCTGCTCCT ACTCCCACCT GCCTGAAGCT GAGGCAAGAT GCAAAGGCGT TGACCCTGGG AAGAGACTTC AAAATGTCAA TGATCCACCG TCACAAATCT TGGATGCCGA TGTCTAGCTT TCTGCAAGGT TGGCCAGTGA CCGAGAACAC TGCACAAGCC AGACCAAGCT CGGACATCAA TGCTGAATGA AGGCCTCCAT ATGAGACCGC GTGACCTGGC ATGAGCTGGA AGTGGGACAA AACTGCTGGA ACAACTGGAT AGGAGATCCA ACAAGGAGCG ACCACGTCAA GCAAATGGGA ATGCCCGACA TCGGGCCCTG GGACCCTGGA AGCCAAAGAT ACAACAAGCA CCACCATCGC GCATCAGCCA ACTCCGGCAC TTGGCAACGA ACCGCCTGGG ACACAGATAC ACATTACCAT CGCGGATGGC TCTCCACGGC CGCGGGCCCG GCGTAATCAA CCTTCGTGAT TGGAGGTGAA CCTACGGCAA CCTTCCCCGA AGGTGAAGTC GCGAGAACTT CCACCGAGAG TGCTGGAGGG CGGTGAAGCT AGGACTACAA TGGACGAGCT GTAGAGGTTT ATGAATGCAA AATAGCATCA TCCAAACTCA GGTGGTTACG TGGAGTTCCG CCCGCCCATT ATTGACGTCA ATCATATGCC ATGCCCAGTA TCGCTATTAC ACTCACGGGG AAAATCAACG GTAGGCGTGT GCTAGCGCTA TTCTCAGCAA GGACCCGGCC CCGGAAGGCG CATGCTGCTG GAGAGTGCAC CAAACTGGTG CATGATCTGG AGCCAAGGAA CATCCAGAAC ACACCGGCCC GAATACGGCT AGACATCGTT CTACCACGCC GTTGGCCGTC TCTGTTGGAG CATGCATGCC GCCCAAGGTG GCGGCTCAGC CAATGCCTGG GATCCGGAGG CCACGAGGCC CACCCTCTCG TGCCCACCAG CTATTATGAC TCTGGGGGCC GACCATTGAC GGAGGGGGCC GGGACTGACC CCTGGCCATC TATGGCGGGC CCACGTGCGG GCAGCACAAT GATCCAGACC GGACCAGCTC TGATCAGCTG CACCAACTAC CAGGACCATC GGAGCAGATG ACTGGGTCCC CCCCCAGGGA GGTAGTGACA AGCAGACCAA GGACGAGCTG CCCCTACACC GCTGTACGGC GGATCCACCG GCCCGACATG CGAGGGCGAG GGAGGGAGCC CAGGGCCTTC GGGCTACTCT CGACATCTCC CCCCCCCAAC GATGTACGTG CGGCGGCCAC GCCCGACTAT CAAGGTGACC GTACAAGTAA TACTTGCTTT TTGTTGTTGT CAAATTTCAC TCAATGTATC CGCAGCGTGA

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Alpha-Actinin
4381 4441 4501 4561 4621 4681 4741 4801 4861 4921 4981 5041 5101 5161 5221 5281 5341 5401 5461 5521 5581 5641 5701 5761 5821 5881 5941 6001 6061 6121 6181 6241 6301 6361 6421 6481 6541 6601 CCGCTACACT CCACGTTCGC TTAGTGCTTT GGCCATCGCC GTGGACTCTT TATAAGGGAT TTAACGCGAA TGTGCGCGGA GAGACAATAA ACATTTCCGT CCCAGAAACG CATCGAACTG TCCAATGATG CGGGCAAGAG ACCAGTCACA CATAACCATG GGAGCTAACC ACCGGAGCTG GGCAACAACG ATTAATAGAC GGCTGGCTGG TGCAGCACTG TCAGGCAACT GCATTGGTAA TTTTTAATTT TTAACGTGAG TTGAGATCCT AGCGGTGGTT CAGCAGAGCG CAAGAACTCT TGCCAGTGGC GGCGCAGCGG CTACACCGAA GAGAAAGGCG GCTTCCAGGG TGAGCGTCGA CGCGGCCTTT GTTATCCCCT TGCCAGCGCC CGGCTTTCCC ACGGCACCTC CTGATAGACG GTTCCAAACT TTTGCCGATT TTTTAACAAA ACCCCTATTT CCCTGATAAA GTCGCCCTTA CTGGTGAAAG GATCTCAACA AGCACTTTTA CAACTCGGTC GAAAAGCATC AGTGATAACA GCTTTTTTGC AATGAAGCCA TTGCGCAAAC TGGATGGAGG TTTATTGCTG GGGCCAGATG ATGGATGAAC CTGTCAGACC AAAAGGATCT TTTTCGTTCC TTTTTTCTGC TGTTTGCCGG CAGATACCAA GTAGCACCGC GATAAGTCGT TCGGGCTGAA CTGAGATACC GACAGGTATC GGAAACGCCT TTTTTGTGAT TTACGGTTCC GATTCTGTGG CTAGCGCCCG CGTCAAGCTC GACCCCAAAA GTTTTTCGCC GGAACAACAC TCGGCCTATT ATATTAACGC GTTTATTTTT TGCTTCAATA TTCCCTTTTT TAAAAGATGC GCGGTAAGAT AAGTTCTGCT GCCGCATACA TTACGGATGG CTGCGGCCAA ACAACATGGG TACCAAACGA TATTAACTGG CGGATAAAGT ATAAATCTGG GTAAGCCCTC GAAATAGACA AAGTTTACTC AGGTGAAGAT ACTGAGCGTC GCGTAATCTG ATCAAGAGCT ATACTGTTCT CTACATACCT GTCTTACCGG CGGGGGGTTC TACAGCGTGA CGGTAAGCGG GGTATCTTTA GCTCGTCAGG TGGCCTTTTG ATAACCGTAT CTCCTTTCGC TAAATCGGGG AACTTGATTA CTTTGACGTT TCAACCCTAT GGTTAAAAAA TTACAATTTA CTAAATACAT ATATTGAAAA TGCGGCATTT TGAAGATCAG CCTTGAGAGT ATGTGGCGCG CTATTCTCAG CATGACAGTA CTTACTTCTG GGATCATGTA CGAGCGTGAC CGAACTACTT TGCAGGACCA AGCCGGTGAG CCGTATCGTA GATCGCTGAG ATATATACTT CCTTTTTGAT AGACCCCGTA CTGCTTGCAA ACCAACTCTT TCTAGTGTAG CGCTCTGCTA GTTGGACTCA GTGCACACAG GCTATGAGAA CAGGGTCGGA TAGTCCTGTC GGGGCGGAGC CTGGCCTTTT TACCGCCATG TTTCTTCCCT GCTCCCTTTA GGGTGATGGT GGAGTCCACG CTCGGTCTAT TGAGCTGATT GGTGGCACTT TCAAATATGT AGGAAGAGTA TGCCTTCCTG TTGGGTGCAC TTTCGCCCCG GTATTATCCC AATGACTTGG AGAGAATTAT ACAACGATCG ACTCGCCTTG ACCACGATGC ACTCTAGCTT CTTCTGCGCT CGTGGGTCTC GTTATCTACA ATAGGTGCCT TAGATTGATT AATCTCATGA GAAAAGATCA ACAAAAAAAC TTTCCGAAGG CCGTAGTTAG ATCCTGTTAC AGACGATAGT CCCAGCTTGG AGCGCCACGC ACAGGAGAGC GGGTTTCGCC CTATGGAAAA GCTCACATGT CAT TCCTTTCTCG GGGTTCCGAT TCACGTAGTG TTCTTTAATA TCTTTTGATT TAACAAAAAT TTCGGGGAAA ATCCGCTCAT TGAGTATTCA TTTTTGCTCA GAGTGGGTTA AAGAACGTTT GTATTGACGC TTGAGTACTC GCAGTGCTGC GAGGACCGAA ATCGTTGGGA CTGTAGCAAT CCCGGCAACA CGGCCCTTCC GCGGTATCAT CGACGGGGAG CACTGATTAA TAAAACTTCA CCAAAATCCC AAGGATCTTC CACCGCTACC TAACTGGCTT GCCACCACTT CAGTGGCTGC TACCGGATAA AGCGAACGAC TTCCCGAAGG GCACGAGGGA ACCTCTGACT ACGCCAGCAA TCTTTCCTGC

Enzyme AatI AatII Acc113I Acc16I Acc65I AccB1I AccB7I AccBSI AccI AccIII AcsI AcyI AfeI AflIII AgeI AhdI Alw21I Alw44I AlwNI Ama87I AocI Aor51HI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AtsI AvaI AviII BalI BamHI BanI

Cuts 4 5 2 1 1 9 2 3 1 2 5 9 1 4 1 3 9 4 4 7 1 1 3 4 5 2 2 2 1 3 9 1 1 7 1 4 3 9

Positions 1843 1858 1951 3067 125 178 261 447 3866 3211 5216 5474 3336 465 1536 1595 2398 2466 3336 3502 4513 5744 3123 3809 2133 4413 4858 3331 770 1793 3007 3320 4252 4737 4748 122 175 258 444 947 1596 3815 3863 5157 596 1358 3272 4324 6585 3357 3081 3273 5697 620 899 2018 2252 2771 3264 5029 5114 6275 895 2248 5025 6271 1572 2274 2841 6176 613 1198 1540 2846 2938 3347 3646 2426 596 2100 2567 3348 895 2248 5025 6271 3007 3320 4252 4737 4748 7 5522 7 5522 2884 5097 3336 3081 3273 5697 620 899 2018 2252 2771 3264 5029 5114 6275 3118 3118 613 1198 1540 2846 2938 3347 3646 5474 868 1483 2335 3455 1502 2570 3351 465 1536 1595 2398 2466 3336 3502 4513 5744

Sequence agg/cct gacgt/c agt/act tgc/gca g/gtacc g/gyrcc ccannnn/ntgg gagcgg gt/mkac t/ccgga r/aatty gr/cgyc agc/gct a/crygt a/ccggt gacnnn/nngtc gwgcw/c g/tgcac cagnnn/ctg c/ycgrg cc/tnagg agc/gct gggcc/c g/tgcac r/aatty at/taat at/taat gaann/nnttc g/gtacc gacnnn/nngtc gwgcw/c gacn/nngtc gacn/nngtc c/ycgrg tgc/gca tgg/cca g/gatcc g/gyrcc

BanII BbeI BbiII BbrPI BbsI BbuI Bbv12I Bbv16II BcgI BclI BcoI BglI BglII BlpI BpiI BpmI

9 1 9 1 3 2 9 3 1 1 7 7 3 2 3 14

Bpu1102I Bpu14I BpuAI BsaAI BsaBI BsaHI BsaI BsaMI BsaOI BsaWI Bse118I Bse21I Bse8I BseAI BseRI BsgI Bsh1285I Bsh1365I BshNI

2 1 3 5 1 9 5 2 6 8 8 1 1 2 7 3 6 1 9

620 1392 2018 2100 2547 2567 3348 3540 4483 1599 122 175 258 444 947 1596 3815 3863 5157 2454 1314 1470 3803 1558 2503 620 899 2018 2252 2771 3264 5029 5114 6275 1314 1470 3803 5181 2691 613 1198 1540 2846 2938 3347 3646 90 212 283 1396 2185 3937 5579 609 902 2069 1675 2638 1314 1470 3803 846 1390 1530 1578 1641 1752 1806 2130 2151 2631 2703 3525 3888 5612 1675 2638 3318 1314 1470 3803 340 1359 2454 3837 4554 4100 122 175 258 444 947 1596 3815 3863 5157 1721 1908 2154 3393 5630 4188 4287 1333 3362 4085 5179 5328 6251 600 770 1332 1793 3357 5401 6232 6379 1193 1592 1682 3238 3357 3805 4449 5612 2426 4100 770 1793 1776 2265 2499 2592 2946 3390 3729 1605 1623 2194 1333 3362 4085 5179 5328 6251 4100 465 1536 1595 2398 2466 3336 3502 4513 5744

grgcy/c ggcgc/c gr/cgyc cac/gtg gaagac gcatg/c gwgcw/c gaagac cgannnnnntgc t/gatca c/ycgrg gccnnnn/nggc a/gatct gc/tnagc gaagac ctggag

gc/tnagc tt/cgaa gaagac yac/gtr gatnn/nnatc gr/cgyc ggtctc gaatgc cgry/cg w/ccggw r/ccggy cc/tnagg gatnn/nnatc t/ccgga gaggag gtgcag cgry/cg gatnn/nnatc g/gyrcc

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Alpha-Actinin
BsiEI BsiHKAI BsiI BsiMI BsmI BsoBI Bsp119I Bsp120I Bsp13I Bsp1407I Bsp143II Bsp1720I Bsp19I BspCI BspEI BspHI BspLU11I BspMI BsrBI BsrBRI BsrDI BsrFI BsrGI BssAI BssSI BssT1I BstBI BstD102I BstDSI BstEII BstH2I BstI BstMCI BstPI BstSFI BstSNI BstX2I 6 9 3 2 2 7 1 3 2 1 9 2 6 1 2 4 2 4 3 1 2 8 1 8 3 9 1 3 11 1 9 3 6 1 8 1 19 1333 3362 4085 5179 5328 6251 620 899 2018 2252 2771 3264 5029 5114 6275 1857 5033 6417 770 1793 4188 4287 613 1198 1540 2846 2938 3347 3646 3318 2096 2563 3344 770 1793 4070 598 1599 2331 2731 3292 4034 4399 4407 6345 1675 2638 360 2219 2762 3167 3368 3719 5328 770 1793 1349 3020 4857 5865 3272 6585 706 1757 2233 2650 2133 4413 4858 4100 5461 5643 1193 1592 1682 3238 3357 3805 4449 5612 4070 1193 1592 1682 3238 3357 3805 4449 5612 1857 5033 6417 360 1073 1613 2219 2762 2855 3167 3368 3719 3318 2133 4413 4858 360 1055 1519 2219 2762 3167 3284 3340 3368 3719 4034 4014 598 1599 2331 2731 3292 4034 4399 4407 6345 1502 2570 3351 1333 3362 4085 5179 5328 6251 4014 1657 2230 3325 3924 4331 5451 6129 6320 340 585 609 902 1502 1790 2069 2264 2570 2600 2837 3137 3351 3983 5050 5067 5835 5847 5933 5944 1299 3045 3519 585 609 902 1502 1790 2069 2264 2570 2600 2837 3137 3351 3983 5050 5067 5835 5847 5933 5944 4082 2426 4082 1675 2638 1193 1592 1682 3238 3357 3805 4449 5612 3343 2846 3347 866 1433 1481 2333 3453 3894 4082 5304 2132 3318 2132 2426 4140 5119 5811 5830 699 921 1929 2096 2226 2564 2621 2935 3593 2047 3966 4557 1359 4601 6483 360 1055 1519 2219 2762 3167 3284 3340 3368 3719 4034 866 1433 1481 2333 3453 3894 4082 5304 4082 687 807 1065 2666 4908 3081 3273 5697 687 807 1065 2666 4908 618 2016 3081 3273 5697 4082 340 360 1073 1613 2219 2762 2855 3167 3368 3719 1843 1858 1951 3067 620 1392 2018 2100 2547 2567 3348 3540 4483 3211 5216 1721 1908 2154 3393 5630 596 4082 828 1072 3435 3858 5015 6063 465 1536 1595 2398 2466 3336 3502 4513 5744 2454 2426 613 1198 1540 2846 2938 3347 cgry/cg gwgcw/c ctcgtg t/ccgga gaatgc c/ycgrg tt/cgaa g/ggccc t/ccgga t/gtaca rgcgc/y gc/tnagc c/catgg cgat/cg t/ccgga t/catga a/catgt acctgc gagcgg gatnn/nnatc gcaatg r/ccggy t/gtaca r/ccggy ctcgtg c/cwwgg tt/cgaa gagcgg c/crygg g/gtnacc rgcgc/y g/gatcc cgry/cg g/gtnacc c/tryag tac/gta r/gatcy MluI MluNI Mph1103I MroI MroNI MscI MslI Msp17I MspA1I MunI Mva1269I NaeI NarI NcoI NdeI NgoAIV NgoMI NheI NotI NsiI NspBII NspI NspV PaeI PaeR7I PflMI PinAI Ple19I PmaCI Pme55I PmlI Ppu10I PpuMI PshBI Psp124BI Psp1406I Psp5II PspAI PspALI PspEI PspOMI PstI PstNHI PvuI PvuII RcaI RsrII SacI 1 4 3 2 1 4 11 9 9 1 2 1 1 6 1 1 1 1 1 3 9 5 1 2 1 2 1 1 1 4 1 3 4 2 2 2 4 2 2 1 3 3 1 1 5 4 1 2 Eco91I EcoICRI EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I EheI ErhI Esp1396I FauNDI FbaI FriOI FspI GsuI 1 2 2 9 1 1 9 3 1 9 2 1 1 9 1 14 3646 4014 618 2016 772 1750 699 921 1929 2096 2226 2564 2621 2935 3593 4014 3320 360 1073 1613 2219 2762 2855 3167 3368 3719 1556 2618 6642 1597 360 1073 1613 2219 2762 2855 3167 3368 3719 3123 3809 234 2691 620 1392 2018 2100 2547 2567 3348 3540 4483 5474 846 1390 1530 1578 1641 1752 1806 2130 2151 2631 2703 3525 3888 5612 598 1599 2331 2731 3292 4034 4399 4407 6345 122 175 258 444 947 1596 3815 3863 5157 915 1128 1440 2052 2673 3332 3909 4201 915 1128 1440 2052 2673 3332 3909 4201 622 3313 4201 122 175 258 444 947 1596 3815 3863 5157 1595 770 1793 3340 2691 687 807 1065 2666 4908 3343 3318 4100 4188 585 609 902 1502 1790 2069 2264 2570 2600 2837 3137 3351 3983 5050 5067 5835 5847 5933 5944 4324 868 1483 2335 3455 1556 2618 6642 770 1793 4449 868 1483 2335 3455 365 634 1054 2611 2774 3166 3370 3718 4926 5285 5444 122 175 258 444 947 1596 3815 3863 5157 1635 2163 2463 2697 2793 3342 5061 6002 6247 4188 4188 4287 4451 1596 360 2219 2762 3167 3368 3719 234 4449 4449 591 4082 1556 2618 6642 1635 2163 2463 2697 2793 3342 5061 6002 6247 675 1558 2503 3276 6589 3318 1558 2503 613 3123 3809 3357 5328 2454 1843 1858 1951 3067 2454 1552 2614 6638 699 2226 2621 2935 7 5522 620 2018 5095 5468 699 2226 2621 2935 2846 3347 2848 3349 4014 2096 2563 3344 1661 2234 3329 591 5328 1635 2163 2463 2697 2793 1349 3020 4857 5865 2132 620 2018 g/gtnacc gag/ctc cctnn/nnnagg rg/gnccy g/gtnacc g/aattc c/cwwgg atgca/t ggc/gcc c/cwwgg ccannnn/ntgg ca/tatg t/gatca grgcy/c tgc/gca ctggag

HaeII Hin1I HincII HindII HindIII HpaI Hsp92I KasI Kpn2I KpnI Ksp22I Ksp632I KspI LspI MamI MfeI MflI

9 9 8 8 2 1 9 1 2 1 1 5 1 1 1 1 19

rgcgc/y gr/cgyc gty/rac gty/rac a/agctt gtt/aac gr/cgyc g/gcgcc t/ccgga ggtac/c t/gatca ctcttc ccgc/gg tt/cgaa gatnn/nnatc c/aattg r/gatcy

BstXI BstYI

3 19

ccannnnn/ntgg r/gatcy

BstZI Bsu36I CciNI CelII Cfr10I Cfr42I Cfr9I CfrI CpoI Csp45I CspI CvnI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1104I Eam1105I EarI Ecl136II EclHKI EclXI Eco105I Eco130I Eco147I Eco24I Eco255I Eco31I Eco47III Eco52I Eco57I Eco64I Eco72I Eco81I Eco88I

1 1 1 2 8 1 2 8 1 1 1 1 4 9 3 3 11 8 1 5 3 5 2 3 1 1 9 4 9 2 5 1 1 6 9 1 1 7

c/ggccg cc/tnagg gc/ggccgc gc/tnagc r/ccggy ccgc/gg c/ccggg y/ggccr cg/gwccg tt/cgaa cg/gwccg cc/tnagg ttt/aaa rg/gnccy cacnnn/gtg gacnnnn/nngtc c/crygg y/ggccr c/ggccg ctcttc gacnnn/nngtc ctcttc gag/ctc gacnnn/nngtc c/ggccg tac/gta c/cwwgg agg/cct grgcy/c agt/act ggtctc agc/gct c/ggccg ctgaag g/gyrcc cac/gtg cc/tnagg c/ycgrg

a/cgcgt tgg/cca atgca/t t/ccgga g/ccggc tgg/cca caynn/nnrtg gr/cgyc cmg/ckg c/aattg gaatgc gcc/ggc gg/cgcc c/catgg ca/tatg g/ccggc g/ccggc g/ctagc gc/ggccgc atgca/t cmg/ckg rcatg/y tt/cgaa gcatg/c c/tcgag ccannnn/ntgg a/ccggt cgat/cg cac/gtg agg/cct cac/gtg a/tgcat rg/gwccy at/taat gagct/c aa/cgtt rg/gwccy c/ccggg ccc/ggg g/gtnacc g/ggccc ctgca/g g/ctagc cgat/cg cag/ctg t/catga cg/gwccg gagct/c

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010

Alleleustrious pmTFP1-Alpha-Actinin
SacII SalI SapI SbfI ScaI SfcI Sfr274I Sfr303I SfuI SmaI SnaBI SphI Sse8387I SseBI SspBI SspI SstI 1 1 1 1 2 8 1 1 1 2 1 2 1 4 1 2 2 3343 3330 2667 2234 3211 5216 1657 2230 3325 3924 4331 5451 6129 6320 613 3343 3318 2848 3349 340 1558 2503 2234 1843 1858 1951 3067 4070 4762 4892 620 2018 ccgc/gg g/tcgac gctcttc cctgca/gg agt/act c/tryag c/tcgag ccgc/gg tt/cgaa ccc/ggg tac/gta gcatg/c cctgca/gg agg/cct t/gtaca aat/att gagct/c SstII StuI StyI Tth111I Van91I VneI VspI XbaI XcmI XhoI XhoII 1 4 9 1 2 4 2 1 5 1 19 3343 1843 1858 1951 3067 360 1073 1613 2219 2762 2855 3167 3368 3719 3118 3123 3809 895 2248 5025 6271 7 5522 4092 686 1381 1491 2760 4056 613 585 609 902 1502 1790 2069 2264 2570 2600 2837 3137 3351 3983 5050 5067 5835 5847 5933 5944 2846 3347 4082 2884 5097 1556 2618 6642 ccgc/gg agg/cct c/cwwgg gacn/nngtc ccannnn/ntgg g/tgcac at/taat t/ctaga ccannnnn/nnnntgg c/tcgag r/gatcy

XmaI XmaIII XmnI Zsp2I

2 1 2 3

c/ccggg c/ggccg gaann/nnttc atgca/t

The following enzymes do not cut: AclNI, AflII, AscI, AvrII, BanIII, BfrI, BlnI, Bsa29I, BscI, BseCI, BsePI, BsiWI, BsmBI, Bsp106I, Bsp68I, BspDI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, Bsu15I, ClaI, Eco32I, EcoRV, Esp3I, FseI, MspCI, NruI, PacI, Pfl23II, PmeI, PshAI, PspLI, SexAI, SfiI, SgfI, SgrAI, SmiI, SpeI, SplI, SrfI, SunI, SwaI, Vha464I

Related products:
Current All ustious Fluorescent Protein Family Members: The founding member is mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
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Basic Vectors Three vectors are available: pNCS-mWasabi, pmWasabi-N and pmWasabi-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.

IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.

Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 Oligo@allelebiotech.com Copyright 2008-2010