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  • Chromatography Paper

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    Sandeep Kumar Thatikonda
    44 vizualizări8 pagini
    Use the technique of paper chromatography to separate a homogeneous mixture into its individual components. Butanol and ammonia (both liquid and vapor) are toxic and are strong skin and eye irritants.

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    Use the technique of paper chromatography to separate a homogeneous mixture into its individual components. Butanol and ammonia (both liquid and vapor) are toxic and are strong skin and eye irritants.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PPT, PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    44 vizualizări8 pagini

    Chromatography Paper

    Încărcat de

    Sandeep Kumar Thatikonda
    Use the technique of paper chromatography to separate a homogeneous mixture into its individual components. Butanol and ammonia (both liquid and vapor) are toxic and are strong skin and eye irritants.

    Drepturi de autor:

    Attribution Non-Commercial (BY-NC)
    Descărcați ca PPT, PDF, TXT sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    din 8

    CHM 101/102

    Chromatography I: Paper
    General Chemistry 101/102 Laboratory Manual University of North Carolina at Wilmington

    Laboratory Manual

    CHM 101/102

    Chromatography I: Paper

    Purpose
     Use the technique of paper chromatography to separate a homogeneous mixture into its individual components.

    Safety Considerations
     Butanol and ammonia (both liquid and vapor) are toxic and are strong skin and eye irritants. Keep the ammonia in the hood and cover all butanol and ammonia solutions.  Dispose of butanol in the waste container in the hood.
    Laboratory Manual

    CHM 101/102

    Chromatography I: Paper

    Procedure
     Place 25 mL of solvent in a 600 mL beaker. Cover the beaker and set it aside.  Obtain a piece of chromatography paper and draw a line 1 cm from the bottom with a pencil.  Place a small spot of each indicator on the line.
    2 mm 25 mL

    1 cm

    Laboratory Manual

    CHM 101/102

    Chromatography I: Paper

    Procedure
     Spot and label each of the four indicators and one of the unknowns. The spots should be about 2 cm apart.  When the spots have dried, re-spot each one. re-

    2 cm

    Laboratory Manual

    CHM 101/102

    Chromatography I: Paper

    Procedure
     When the spots have dried, form the paper into a cylinder with the spots facing out. Staple the edges together being careful to keep them straight and not allowing them to touch.  Place the cylinder into the 600 mL beaker and replace the cover. Be sure the cylinder is not touching the sides of the beaker.
    Laboratory Manual

    CHM 101/102

    Chromatography I: Paper

    Procedure
     Let the chromatogram develop until the solvent is 2 cm from the top of the paper.  Remove the chromatogram from the beaker and immediately mark the solvent front with a pencil.  Allow the chromatogram to dry before going to the next step.

    Laboratory Manual

    CHM 101/102

    Chromatography I: Paper

    Procedure
     Take the chromatogram to the hood and lightly mist it with water. Place it in the ammonia chamber.

     Remove the cylinder from the ammonia chamber and unroll it. Immediately circle the colored regions with a pencil.
    Laboratory Manual

    CHM 101/102

    Chromatography I: Paper

    Procedure
     Determine the RF values for each colored spot in the knowns and the unknown.

    RF(a) =

    a d

    d c

     Use your computed RF values to identify the components of your unknown.


    Laboratory Manual

    b a

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