Gram-positive Cocci

Gram-positive Cocci
Catalase Test

Catalase Negative Flow Chart

Streptococcaceae Hemolysis

+
Micrococcaceae

Beta
GpC Strep GpA Strep (S. pyogenes)

Alpha
Enterococcus Strep mitis

Bacitracin Sensitivity

Bile Esculin Test

+
GpA Strep (S. pyogenes)

GpC Strep

+
Enterococcus

Strep mitis Strep pneumoniae

Optichin Sensitivity

+
Strep pneumoniae

Strep mitis

Gram-positive Cocci

Gram-positive Cocci

Catalase Test

Catalase Positive Flow Chart

+
Micrococcaceae

Streptococcaceae

Coagulase Test

Mannitol Fermentation Test

Hemolysis

+
Staphylococcus aureus

Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

+
Staphylococcus aureus

Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

Beta
Staphylococcus aureus

Alpha
Staphylococcus epidermidis Micrococcus luteus Micrococcus roseus

Growth on Mannitol Salt Agar Test (MSA)

Pigmentation Observation

Growth on MSA or Pigmentation Observation

+
Staphylococcus aureus Staphylococcus epidermidis

Micrococcus luteus Micrococcus roseus

Grey/White
Staphylococcus epidermidis

Golden/White
Staphylococcus aureus

Mustard Yellow

Pale Pink/Rose

Micrococcus luteus

Micrococcus roseus

See Pigmentation Observation to differentiate

Gram-negative Rods

Gram-negative Rods

Lactose Positive Flow Chart

+

Lactose Test
(KIA or Carbohydrate Fermentation Test)

IMViC Tests

++-E. coli

--++

Motility

+
Enterobacter aerogenes

Klebsiella

Gram-negative Rods

Gram-negative Rods

Lactose Test
(KIA or Carbohydrate Fermentation Test)

Lactose Negative Flow Chart

_

Oxidase Test

Gelatin Test

H2S (KIA) Test

+ Pseudomonas aeruginosa

Alcaligenes faecalis

Shigella flexneri

Urea Test

+ Proteus vulgaris

Salmonella typhimurium