Pharmaceutical Biochemistry, 341C NAME, Exam 2, Dr. Whitman, 100pts November 10, 2003 PART I - True or False (circle T or F) (2 pts each) MM tT) xT a Activation of chymotrypsinogen involves the which will accommodate oxyanion hole. — 3 1 oP H—}—on HO——H H——on at CH,OH ;—OH positively charged side chains, as well as the completion of hte Jone Proteases share the same two amino acids in their active sites. =F CHARLES StKANG (Print legibly) (DO NOT PUT YOUR SSN ON THIS EXAM) wy Fora a q> formation of the substrate specificity (Ws & Ors J aqueous solution, the monosaccharide shown below readily forms two cyclic-tings.--<) ye & F Hydrolysis of the glycosidic bond of the reducing sugar lactose (below) produces galactose and glucose and hydrolysis of the produce fructose and glucose. HoH CHO -q ft ao on wk & Ho H HO Lactose The conversion of glucose to pyruvate. b molecules, but generates four ATP molecule: x QF glycosidic bond of the non-reducing sugar sucrose 1H,0H HH HO. HO Sucrose y the glycolytic enzymes requires two ATP > #aT: ©) ‘The flavin ring structures below correctly shows the oxidized and reduced forms. + a:=, t CHs N. a cH CH in cH ¥ reduced “ increase in the concentration of AMP only affects one of the enzymes. © sé ; T®): An increase in concentration of ATTP will afect three of the glycolytic enzymes whereas an 0 Reactions A and B are examples of oxidation reactions whereas reaction C represents a reduction reaction. "= I “Ne aremeeqiation A. ()/ The anaerobic fermentation of pyruvate in yeast involves two enzymes and results in the production of CO, and NAD® 2... DO AT Wve active and stable form of chymotrypsin (a-chymotrypsin) released by the trypsin. — digestion of chymotrypsinogen has three amino-termini.™© ease 12 ee alee are T-/(G) ‘soniazid (shown below) can cause deficiencies of nicotinic acid and pyridoxine... we ' R ~ ‘—NHNH,, © I Z |& T 1) Two moles of NAD® are produced in the pyruvate dehydrogenase reaction, which takes place in the cytosol, per mole of glucose shuttled through the glycolytic pathway. a: cae © The reaction shown below is catalyzed by G-lactamase, a serine, protease, which plays an important role in bacterial resistance to antibiotics. -saser-, euwe 17 oer K i é DET @y Fructose-1,6-bisphosphate is a central molecule in metabolism. It is used in the synthesis of many molecules and all carbohydrates go through this molecule on their way to pyruvate, cA AS TG Lipid soluble vitamins dike Vitamins A and D) can be administered in a single dose, v whereas water-soluble vitamins much be supplied more frequently. The primary reason for this difference is due to the greater toxicity of the water-soluble vitamins (when administered in single doses). FAwe : Part IL. Multiple Choice (no correct answer, one, two or more correct answers) (4 pts each) 16. Three steps in glycolysis are shown below. The letters above the arrows correspond to an enzyme- catalyzed reaction. Which of the following statements is/are TRUE about these reactions? A B c Glucose -> glucose-6-phosphate -» Fructose-6-phosphate —> Fructose-1,6-diphosphate (77 Reseton A takes place in the liver and muscle, but there are substantially different properties between the two reactions. 2: Reaction C is the key regulatory step in glycolysis. Reaction A is shared by both glycolysis and gluconeogenesis, x D. Reaction B generates ADP. > 17. Which of the following vitamins is/are correctly paired with a reaction carried out by its coenzyme? ‘ ‘ il ~ Ww Loup fv (pe Thiamine: cup —c0? ——> cacuS, 7° FUP hn, AD v BO Pantothenic acid: CH,CH,oH ——> cH,C—H lo 2 © ® .) Riboflavin: 0,0cH,cH,c0;, ——> O.c—cH=cH—C® Beanie e) Pyridoxine: COrten