Aim of experiment

To determine the concentration of an external solution that is isotonic to cell sap of potato cells. What is the concentration of external solution which is isotonic to cell sap of potato cells. The higher the concentration of external solution, the lower the percentage change in the final mass of potato strips. Concentration of external solution Percentage change in the final mass of potato strips. Volume of solution A fresh potato, distilled water, tissue paper, 0.1M, 0.2M, 0.3M, 0.4M, 0.5M, and 0.6M sucrose solutions. Small beakers, a cork borer, a scapel, a pair of forceps, a ruler, an electronic balance, and stopwatch. The percentage change in the final mass of potato strips is measured using a electronic balance and recorded in the table result. 1. Seven small beakers is label as dw, 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6. 2. Each small beakers from dw - 0.6 is filled with distilled water, 0.1M sucrose solution , 0.2M sucrose solution, 0.3M sucrose solution, 0.4M sucrose solution, 0.5M sucrose solution, 0.6M sucrose solution. 3.Use a cork borer to remove seven cylindrical strips from a potato. 4.A scalpel is use to trim the potato to a length of 1cm each. 5.Each potato strip is dry with tissue paper before weighing it. 6.The potato strips is weigh three by three for each beaker for the initial mass of potato strips. 7.The set that contain three potato strips each is put on each beaker that label dw until 0.6. 8.Remove each potato strips from its respective beakers after 20 minit pass and dry it, and weigh it again for final mass of the potato strips. 9.The result is recorded.
Percentage change in mass of potato strips = Final Mass Initial Mass --------------------------------- x 100% Initial mass of potato

Problem statement

Hypothesis

Manipulated variable Responding variable Constant variable Materials

: : : :

Apparatus

Technique

Procedure

10.A graph of the percentage change in mass of potato strips against the concentration of the external(sucrose) solution is plot. Precaution step : Do not place the potato strips on the tissue more than 50 seconds.

Teacher signature

_________________________ (NOR HANANIE BT. ABDULLAH)

Result

:
Concentration of external solution 0.0
(distilled water)

Mass of Potato strip (g) Initial 2.5 2.54 2.18 2.64 2.83 2.61 2.53 Final 2.72 2.74 2.38 2.84 3.00 2.44 2.29

Difference in Mass 0.22 0.20 0.20 0.20 0.17 - 0.17 - 0.24

Percentage (%) difference 8.8 7.9 9.2 7.6 6.0 - 6.5 - 9.5

0.1 0.2 0.3 0.4 0.5 0.6

Discussion

The result show that at external concentration of less 0.45M the cell sap of potato tissue is hypertonic to external(sucrose) solution. When the external solution is at 0.45M, the sap cell of potato tissue is isotonic to external(sucrose) solution. If the external(sucrose) concentration is greater than 0.45M, the cell sap of potato tissue is hypotonic to external(sucrose) solution. So the texture and appearance on less than 0.45M is turgid and expanded. The expanded is hard to see wioth naked eye but we can fell that the potato strip is getting harder. On the 0.45M solution, the potato strip maintain it shape and when greater than 0.45M the potato strip become flaccid and plasmoly. The axes on the graph represent the change in percentange of the mass of the potato strip. The x axis represent external solution and the y axis represent the percentage. The intersect at the zero of the y axis the the way to determine the isotonic of solution to the cell sap of potato. In the less then 0.45M ,the osmosis occur as the water diffuse into the cell sap. In the greater then 0.45M ,the osmosis occur as the water diffuse out to the cell sap. In the 0.45M solution, no net water movement. Based on the graph, it support the hypothesis and the concentration of the cell sap of potato tissueis estimated to be 0.45M.The hypothesis is accepted.

Conclusion